Inhibitory effects of Choto-san (Diao-teng-san), and hooks and stems of Uncaria sinensis on free radical-induced lysis of rat red blood cells

Sekiya, Nobuyasu; Shimada, Yutaka; Shibahara, Naotoshi; Takagi, Shinobu; Yokoyama, Kôichi; Kasahara, Yuji; Sakakibara, Iwao; Terasawa, Katsutoshi

doi:10.1078/094471102321616454

Cited by 22 publications

(12 citation statements)

References 14 publications

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“…The extract inhibited the hemolysis of RBC induced by 2,2-zao-bis(2-amidinopropane)dihydrochloride (APPH) in a dose-dependent manner from 50 to 1000 mg/mL ( Sekiya et al, 2002). Apea-Bah et al (2009) have reported that the ethanol extract and ethyl acetate extract of the U. gambir dried leaves showed significantly higher (p o0.01) DPPH inhibitory activity than the aqueous soluble.…”

Section: Antioxidant Activitiesmentioning

confidence: 97%

Medicinal uses, phytochemistry and pharmacology of the genus Uncaria

Zhang

Zhao

et al. 2015

Journal of Ethnopharmacology

160

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Section: Antioxidant Activitiesmentioning

confidence: 97%

Medicinal uses, phytochemistry and pharmacology of the genus Uncaria

Zhang

Zhao

et al. 2015

Journal of Ethnopharmacology

160

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“…[8][9][10][11] The addition of AAPH to the suspension of RBC causes the oxidation of lipids and proteins in the cell membrane, thereby inducing hemolysis. It is known that AAPH-induced hemolysis in RBC is a function of incubation time and proportional to the concentration of free radicals.…”

Section: Inhibitory Effects Of Stephania Tetrandra S Moore On Free Rmentioning

confidence: 99%

Inhibitory Effects of Stephania tetrandra S. MOORE on Free Radical-Induced Lysis of Rat Red Blood Cells

Sekiya

Hikiami

Yokoyama

et al. 2005

Biological & Pharmaceutical Bulletin

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Stephania tetrandra (ST) has been used as an analgesic drug for arthritis for thousands of years in China. ST has been reported to have anti-inflammatory 1,2) and immunosuppressive 3) properties in vitro and in experimental animals. This study was performed to clarify the role of ST from an antioxidative point of view.In the present study, we examined the effects of ST on scavenging activities of reactive oxygen species (ROS) by means of electron spin resonance (ESR) method, and on red blood cell (RBC) hemolysis induced by an azo free-radical initiator in healthy volunteers. The ESR method was used for the measurement of O 2 Ϫ · and HO · scavenging activities of ST. RBC hemolysis was used for determination of the antioxidative effect of ST, and the influence of ST on RBCs, which have the most important role in hemorrheology.Furthermore, we hypothesized that ingestion of ST extract (ST.E) would further strengthen the antioxidative effect of plasma, which already has a function of reducing the free radical-induced tissue damage in cerebrovascular disease and ischemic heart disease, if the responsible compounds in ST could be absorbed and circulated in blood. Therefore, the present study examined the effect on plasma antioxidative activity after a gavage-dose of ST.E in rats. MATERIALS AND METHODS Preparation of Drugs ST.E was prepared fromStephania tetrandra (Chinese origin) purchased commercially (Uchida Wakanyaku Co. Ltd., Tokyo, Japan). The extract was obtained by boiling the herb in water for 50 min and then freeze-drying into a resultant powder. We obtained 10.67 g of extract from 100 g of raw material.This powder extract was dissolved in phosphate buffered saline (PBS, pHϭ7.4).Reagents Tetrandrine (TET), fangchinoline (FAN) and cyclanoline (CYC) were the products of Tsumura & Co. (Tokyo, Japan). Their contents in ST.E were 2%, 1% and 1%, respectively. The chemical structures of TET, FAN and CYC are illustrated in Fig. 1. The purity of each compound was examined by HPLC using a photodiode array detector.ESR Assay for O 2 ؊ · and HO · Scavenging Activities of ST.E Measurement of O 2 Ϫ · and HO · scavenging activities was performed as described previously 4) with slight modification. ST.E was diluted to 0.2, 2.0, and 20.0 mg/ml with PBS. O 2 Ϫ · and HO · scavenging activities of each sample (50 ml) were assessed by electron spin resonance (ESR) technique 5) and then IC 50 (Inhibition Concentration 50%) was calculated.In this experiment, O 2 Ϫ · was generated from a hypoxanthine (HPX)-xanthine oxidase (XOD) reaction system in PBS.6) Briefly, 15 ml of 9.2 M 5,5-dimethyl-1-pyrroline Noxide (DMPO) (LABOTEC, Tokyo, Japan), 50 ml of 2 mM HPX (Sigma, St. Louis, MO, U.S.A.), 35 ml of 5.5 mM diethylenetriamine-N,N,NЈ,NЉ,NЉ-pentaacetic dianhydride (DETA- Crude preparations of Stephania tetrandra S. MOORE (ST), a traditional herbal medicine, have been used safely for arthritis and silicosis in China. In this study, we demonstrated that ST in vitro protects red blood cells from 2,2-azo-bis (2-amidinopropane) dihydrochlorid...

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“…The 'chotosan' preparation without 'chotoko' was inactive (Mimaki et al, 1997). An extract from the stems and hooks showed anti-haemolytic activity in rats (Sekiya et al, 2002). Geissoschizine methyl ether, corynantheine, and dihydrocorynantheine decreased specific [ 3 H]5-hydroxytryptamine binding to membrane preparations from rat brain (Kanatani et al, 1985).…”

Section: U Sinensis (Oliv) Havilmentioning

confidence: 98%

Advances in chemistry and bioactivity of the genus Uncaria

Laus¹

2004

Phytotherapy Research

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The alkaloids of the pantropical genus Uncaria (Rubiaceae) were reviewed extensively by Phillipson et al. in (1978) Since then a host of papers with new results has been published. The compounds discovered are from three major groups: (1) alkaloids; (2) terpenoids; and (3) flavonoids. Some highly unusual structures have been reported. Although there are at least 35 species of Uncaria known, recent research focussed on a relatively small number of species. During recent years bioactivity of extracts and pure compounds isolated from Uncaria species have been increasingly investigated. These include anticonvulsive, antiinflammatory, antimutagenic, antioxidant, cytoprotective, hypotensive, and immunoregulatory effects. The literature since 1978 is reviewed and 133 references are given.

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Inhibitory effects of Choto-san (Diao-teng-san), and hooks and stems of Uncaria sinensis on free radical-induced lysis of rat red blood cells

Cited by 22 publications

References 14 publications

Medicinal uses, phytochemistry and pharmacology of the genus Uncaria

Medicinal uses, phytochemistry and pharmacology of the genus Uncaria

Inhibitory Effects of Stephania tetrandra S. MOORE on Free Radical-Induced Lysis of Rat Red Blood Cells

Advances in chemistry and bioactivity of the genus Uncaria

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