Inhibitory effects of cocoa tea (Camellia ptilophylla) in human hepatocellular carcinoma HepG2 in vitro and in vivo through apoptosis

Xiao, Yang; Wang, Yuan Yuan; La, Kai Kai; Li, Peng; Song, Xia; Shi, Xiang gang; Zhu, Xiao Feng; Leung, Ping Chung; Ko, Chun‐Hay; Ye, Chuang Xing

doi:10.1016/j.jnutbio.2011.05.010

Cited by 33 publications

(15 citation statements)

References 39 publications

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“…Peng et al also demonstrated the potential of cocoa tea extract to inhibit prostate cancer cell proliferation and tumour growth in vivo and in vitro [23]. More recently, our laboratory showed that administration of cocoa tea to HepG2 xenograft nude mice resulted in dose-dependent reduction in tumour growth via caspase-3-regulated pathway [24]. Despite having high catechins content, the beneficial effects of cocoa tea on cardiovascular health have not been studied in details.…”

Section: Introductionmentioning

confidence: 99%

Effect of Dietary Cocoa Tea (Camellia ptilophylla) Supplementation on High-Fat Diet-Induced Obesity, Hepatic Steatosis, and Hyperlipidemia in Mice

Xiao

Wat

Wang

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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Recent studies suggested that green tea has the potential to protect against diet-induced obesity. The presence of caffeine within green tea has caused limitations. Cocoa tea (Camellia ptilophylla) is a naturally decaffeinated tea plant. To determine whether cocoa tea supplementation results in an improvement in high-fat diet-induced obesity, hyperlipidemia and hepatic steatosis, and whether such effects would be comparable to those of green tea extract, we studied six groups (n = 10) of C57BL/6 mice that were fed with (1) normal chow (N); (2) high-fat diet (21% butterfat + 0.15% cholesterol, wt/wt) (HF); (3) a high-fat diet supplemented with 2% green tea extract (HFLG); (4) a high-fat diet supplemented with 4% green tea extract (HFHG); (5) a high-fat diet supplemented with 2% cocoa tea extract (HFLC); and (6) a high-fat diet supplemented with 4% cocoa tea extract (HFHC). From the results, 2% and 4% dietary cocoa tea supplementation caused a dose-dependent decrease in (a) body weight, (b) fat pad mass, (c) liver weight, (d) total liver lipid, (e) liver triglyceride and cholesterol, and (f) plasma lipids (triglyceride and cholesterol). These data indicate that dietary cocoa tea, being naturally decaffeinated, has a beneficial effect on high-fat diet-induced obesity, hepatomegaly, hepatic steatosis, and elevated plasma lipid levels in mice, which are comparable to green tea. The present findings have provided the proof of concept that dietary cocoa tea might be of therapeutic value and could therefore provide a safer and cost effective option for patients with diet-induced metabolic syndrome.

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Section: Introductionmentioning

confidence: 99%

Effect of Dietary Cocoa Tea (Camellia ptilophylla) Supplementation on High-Fat Diet-Induced Obesity, Hepatic Steatosis, and Hyperlipidemia in Mice

Xiao

Wat

Wang

et al. 2013

Evidence-Based Complementary and Alternative Medicine

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“…When the literature is reviewed, it will be seen that the quantity of HepG2 cell used in the in vitro studies are different such as 10 3 cells/well. 29 For the determination of the cell quantity, our method is far away from subjectivity and in this study, 5 x 10 3 cells/well cells are used.…”

Section: Discussionmentioning

confidence: 99%

The Role of Acetylsalicylic Acid as an Assistant Drug in the Management of Hepatocellular Carcinoma

Alaçam¹

2012

UHOD

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Glucose regulated protein 78 (Grp78) is a chaperon which acts in the protein folding. Cell-surface Grp78 is present on the surface of different type cancer cells. There are evidences that acetylsalicylic acid (ASA) provides advantages when combined with other chemotherapeutic agents in cancer patients. Resistance is an important problem in cancer treatment and etoposide is a drug which develops resistance against itself. Our purpose in this study is to see how the ASA, either used alone or combined with etoposide, affect on the HepG2 cell viability and to investigate the relationship of this viability with the cell-surface Grp78. Thus 12 different groups were assigned. Control group, 0.5 µg/ml etoposide group, 2.5, 5, 10 mM ASA groups, 10 mM 2-deoxyglucose (2-DG) + 0.5 µg/ml etoposide group and the groups added to this group with 2.5, 5 and 10 mM ASA respectively, 10 mM 2-DG +2.5 mM ASA group, 10 mM 2-DG + 5 mM ASA group, 10 mM 2-DG + 10 mM ASA group. MTT test was applied for the follow up of the viability of the cells. When the viability in etoposite group was %84.5, the viability in 2-DG+etoposit group was %75.4. This shows that 2-DG decreases the viability of the cells a little. Also, when ASA is applied alone or combined with 2-DG, it caused decrease in the cell viability but this effect was more significant when combined with 2-DG. When etoposide and ASA were combined, the decrease in high-dose viability was more significant. Cell surface Grp78 levels were higher in the group in which 2-DG and ASA were combined each other than the group in which ASA was applied alone. All these results show that cell-surface Grp78 is important for ASA showing its effect. New treatment protocols focused on the cell-surface Grp78 in cancer treatment can be revealed by further studies.Keywords: Grp78, Cell-surface Grp78, HepG2, Etoposide, Acetylsalicylic acid ÖZET Hepatosellüler Karsinoma Tedavisinde Yard›mc› bir ‹laç Olarak Asetilsalisilik Asit'in RolüGlucose regulated protein 78 (Grp78) protein katlanmas›nda rol oynayan bir flaperondur. Cell-surface Grp78 farkl› türde kanser hüc-relerinin yüzeyinde bulunmaktad›r. Acetylsalicylic acid (ASA)'n›n kanser hastalar›nda kullan›m›n›n fayda sa¤lad›¤›na dair kan›tlar vard›r. Kanser tedavisinde direnç önemli bir problemdir ve etoposide kendisine direnç geliflen bir ilaçt›r. Bizim bu çal›flmadaki amac›m›z etoposide ile kombine olarak ya da tek bafl›na kullan›lan ASA'n›n HepG2 hücre viabilitesi üzerine nas›l etki etti¤ini görmek ve bu canl›l›-¤›n cell-surface Grp78 ile iliflkisini araflt›rmakt›r. Bu amaçla 12 farkl› grup oluflturuldu. Kontrol grubu, 0.5 µg/ml etoposid grubu, 2.5, 5, 10 mM ASA gruplar›, 10 mM 2-deoxyglucose (2-DG) + 0.5 µg/ml etoposid içeren grup ve bu gruba s›ras›yla 2.5, 5 ve 10 mM ASA eklenen gruplar, 10 mM 2-DG +2.5 mM ASA grubu, 10 mM 2-DG + 5 mM ASA grubu, 10 mM 2-DG + 10 mM ASA grubu. Hücre-lerin canl›l›klar›n›n izlenmesi içinde MTT testi yap›ld›. Sadece etoposit verilen grupta canl›l›k %84.5 iken, 2-DG+etoposit verilen grupta canl›l›k %75.4 kadard›. 2-DG'n...

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“…Recent study showed cocoa tea changed the ambulatory behaviours combined administration with green tea (He et al ., ). Our laboratory also found that cocoa tea had the inhibitory ability against prostate cancer and hepatocellular carcinoma both in vivo and in vitro (Peng et al ., ; Yang et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Subacute oral toxicity of cocoa tea (Camellia ptilophylla) water extract in SD rats

Zhou

Yang

et al. 2015

Int J of Food Sci Tech

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Camellia ptilophylla, known as cocoa tea, is a natural decaffeinated tea plant which has been proved with potential bioactivities. This study was carried out to evaluate the safety of green cocoa tea water extract (CWE) by determining its potential toxicity after subacute administration in SD rats. In the subacute toxicity study, oral administration of CWE up to 800 mg kg À1 body weight day À1 for twenty-eight consecutive days did not cause either mortality or toxicity in SD rats, regardless of gender. There were no adverse effects in body weight, food consumption, relative organ weight, haematological parameters, clinical chemistry, gross pathology and histopathology between treatment and control groups. Several parameters (e.g. neutrophils%, Ca 2+ , total Ca) in CWE-treated rats with high dosage were significantly different from the control groups, but still in a safety level. A dose of 800 mg kg À1 day À1 was identified as the no observed adverse effect level (NOAEL) in this study.

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Inhibitory effects of cocoa tea (Camellia ptilophylla) in human hepatocellular carcinoma HepG2 in vitro and in vivo through apoptosis

Cited by 33 publications

References 39 publications

Effect of Dietary Cocoa Tea (Camellia ptilophylla) Supplementation on High-Fat Diet-Induced Obesity, Hepatic Steatosis, and Hyperlipidemia in Mice

Effect of Dietary Cocoa Tea (Camellia ptilophylla) Supplementation on High-Fat Diet-Induced Obesity, Hepatic Steatosis, and Hyperlipidemia in Mice

The Role of Acetylsalicylic Acid as an Assistant Drug in the Management of Hepatocellular Carcinoma

Subacute oral toxicity of cocoa tea (Camellia ptilophylla) water extract in SD rats

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