Inhibitory effects of dihydroxyacetone on Gluconobacter cultures

Ohrem, H. Leonhard; Voβ, Harald

doi:10.1007/bf00127438

Cited by 8 publications

(7 citation statements)

References 5 publications

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“…3, illustrates the extended deceleration phase when aeration rate was increased. This may be due to the build-up of DHA within the beads, which inhibited the cell growth and further product formation [14].…”

Section: ) Effect Of Aeration Ratementioning

confidence: 99%

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Bioconversion of Glycerol to Dihydroxyacetone by Immobilized Gluconacetobacter Xylinus Cells

Black¹,

Nair²

2013

IJCEA

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Section: ) Effect Of Aeration Ratementioning

confidence: 99%

“…Oxidation of glycerol to DHA by G.oxydans is inhibited by high concentrations of both DHA and glycerol [14], [15]. Besides, the microbial oxidation of glycerol to DHA has a high oxygen requirement [15], [16].…”

Section: Introductionmentioning

confidence: 99%

Bioconversion of Glycerol to Dihydroxyacetone by Immobilized Gluconacetobacter Xylinus Cells

Black¹,

Nair²

2013

IJCEA

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“…It inhibits cell growth and further product formation. Viability of Gluconobacter decreases exponentially with time of exposure to DHA and with exposure to concentrations of over 5% DHA (Ohrem and Voss, 1995a). DHA blocks microbial development first and then inhibits glycerol oxidation, probably by interacting with the amine function at the active site of glycerol dehydrogenase.…”

Section: Product Inhibitionmentioning

confidence: 97%

The GenusGluconobacterand Its Applications in Biotechnology

Macauley

McNeil

Harvey

2001

Critical Reviews in Biotechnology

106

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Organisms of the genus Gluconobacter have been widely utilized within the biotechnology industry for many decades, due to their unique metabolic characteristics. The metabolic features that render Gluconobacter so useful in biotransformation processes, vitamin synthesis, and, as the biological element in sensor systems, are critically evaluated, and the relevance of recent biochemical genetic studies to current and future industrial Gluconobacter processes is discussed. The impact of recombinant gene technology on the status of Gluconobacter processes and the potential use of such techniques in clarifying aspects of the physiology of Gluconobacter is reviewed.

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“…Stationary Growth Kinetics. It is known from literature that small concentrations of glycerol have a limiting effect on cell growth and that large concentrations of glycerol inhibit cell growth (2, 5, 6). Therefore in this work, the concentration of glycerol was controlled within a range of 10–20 g L −1 at all times by applying fed‐batch operation in order to avoid substrate limitaton and inhibition.…”

Section: Description Of the Segregated Model With Transient Growth Ratesmentioning

confidence: 99%

“…This, however, does not apply to cultures that are governed by product inhibition. In a previous work, it was shown that G. oxyd ans was strongly product‐inhibited and the physiological state of the cells changed significantly (5). In this case, the above‐mentioned models cannot yield an adequate description.…”

Section: Introductionmentioning

confidence: 99%

Development of a Transient Segregated Mathematical Model of the Semicontinuous Microbial Production Process of Dihydroxyacetone

Bauer

Hekmat

2006

Biotechnol. Prog.

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For the mathematical description of the semicontinuous two-stage repeated-fed-batch fermentation of dihydroxyacetone (DHA), a novel segregated model incorporating transient growth rates was developed. The fermentation process was carried out in two stages. A viable, not irreversibly product-inhibited culture was maintained in the first reactor stage until a predetermined DHA threshold value was reached. In the second reactor stage, high final product concentrations of up to 220 g L(-1) were reached while the culture was irreversibly product-inhibited. The experimentally observed changes of the physiological state of the culture due to product inhibition were taken into account by introducing a segregation into the mathematical model. It was shown that the state of the cells was dependent on the current environment and on the previous history. This phenomenon was considered in the model by utilizing delay time equations for the specific rates of growth on the primary and the secondary substrate. A comparison with reproducible measurements gave a good correlation between computation and experiment. The mathematical model was validated using independent own experimental data. A comparison with a stationary and nonsegregated model demonstrated the essential improvements of the novel model. It was deduced from the model calculations that high product formation rates of 3.3-3.5 g L(-1) h(-1) as well as high final DHA concentrations of 196-215 g L(-1) can be obtained with a residual broth volume in the first reactor stage of 2% and a DHA threshold value in the range of 100-110 g L(-1).

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Inhibitory effects of dihydroxyacetone on Gluconobacter cultures

Cited by 8 publications

References 5 publications

Bioconversion of Glycerol to Dihydroxyacetone by Immobilized Gluconacetobacter Xylinus Cells

Bioconversion of Glycerol to Dihydroxyacetone by Immobilized Gluconacetobacter Xylinus Cells

The GenusGluconobacterand Its Applications in Biotechnology

Development of a Transient Segregated Mathematical Model of the Semicontinuous Microbial Production Process of Dihydroxyacetone

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