Inhibitory effects of (−)-epigallocatechin gallate on the life cycle of human immunodeficiency virus type 1 (HIV-1)

Yamaguchi, Koushi; Honda, Mitsuo; Ikigai, Hajime; Hara, Yukihiko; Shimamura, Tadakatsu

doi:10.1016/s0166-3542(01)00189-9

Cited by 201 publications

(146 citation statements)

References 50 publications

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“…It has been reported to inhibit various viruses through a number of mechanisms. The documented anti-viral activities of EGCG include the inhibition of the influenza virus, 18) human immunodeficiency virus type 1 (HIV-1), 19,20) Epstein-Barr virus, 21,22) herpes simplex virus, 23) and hepatitis B virus. 24) In vitro, EGCG inhibited various viral enzymes, such as adenovirus protease adenain, 25) influenza A RNA polymerase, 26) and HIV-1 integrase.…”

Section: Discussionmentioning

confidence: 99%

A Cell-Based, Microplate Colorimetric Screen Identifies 7,8-Benzoflavone and Green Tea Gallate Catechins as Inhibitors of the Hepatitis C Virus

Fukazawa

Suzuki

Wakita

et al. 2012

Biological & Pharmaceutical Bulletin

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We describe a cell-based, microplate colorimetric screen for anti-hepatitis C virus (HCV) drugs that exploits the HCV-JFH1 viral culture system. Antiviral activity was assessed by measuring protection against the HCV-JFH1-induced cytopathic effect (CPE) in Huh7.5.1 cells using the 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) viability assay. The use of serum-free medium substantially sensitized Huh7.5.1 cells to HCV-induced CPE, causing sufficient cell death to perform colorimetric assays for anti-HCV activity in 96-well plates. As a proof of concept, we carried out a pilot screen of an inhibitor library and identified cyclosporin A and tamoxifen, two compounds with reported anti-HCV activity. Using the assay, we discovered the anti-HCV properties of the plant flavonoids epigallocatechin gallate (EGCG) and 7,8-benzoflavone (α-naphthoflavone). Other gallate-type catechins and flavones also displayed anti-HCV activity, but 5,6-benzoflavone (β-naphthoflavone), flavanone, and non-gallate catechins were inactive. EGCG apparently acted mainly on HCV entry, although it may also block other steps. In contrast, 7,8-benzoflavone was presumed to inhibit later stages of the HCV life cycle. This assay is simple, reliable and cost-effective; does not require any specially engineered cell lines or viruses; and should be useful in the identification of compounds with anti-HCV activity. Key words hepatitis C virus; 7,8-benzoflavone (α-naphthoflavone); epigallocatechin gallateMore than 170 million people worldwide are chronically infected with the hepatitis C virus (HCV) and are at risk for developing liver diseases such as cirrhosis and hepatocellular carcinoma. Vaccines against HCV are not currently available; furthermore, the standard interferon/ribavirin combination therapy is not effective in approximately half of HCV-infected patients, and it has considerable side effects.1,2) Thus, there is an obvious and urgent need for new agents that can enhance or replace current HCV therapies.Screening programs using HCV replicon-based systems have successfully identified compounds that act on HCV RNA replication. However, replicon systems do not reproduce the entire HCV life cycle, and they cannot isolate inhibitors of many important steps such as viral entry, assembly, and egress. HCV cell culture infection models that recapitulate the entire viral life cycle in vitro have greatly enhanced the opportunity for HCV drug discovery. Several reports, including ours, have demonstrated that this model can overcome the limitations of the HCV replicon system and enable the discovery of compounds that target various stages of the HCV life cycle. [3][4][5][6][7][8] Using the HCV cell culture system, we previously developed a tube-capture-reverse transcription-polymerase chain reaction (RT-PCR) assay for screening HCV inhibitors and identified bisindolylmaleimides and indolocarbazoles as inhibitors of HCV replication.4) Here, we describe another screening method for the detection of anti-HCV activity. This assay measures t...

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Section: Discussionmentioning

confidence: 99%

A Cell-Based, Microplate Colorimetric Screen Identifies 7,8-Benzoflavone and Green Tea Gallate Catechins as Inhibitors of the Hepatitis C Virus

Fukazawa

Suzuki

Wakita

et al. 2012

Biological & Pharmaceutical Bulletin

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“…Compounds isomeric with catechin dimers were detected for precursor ions at m/z 577 (8-11), 593 (12)(13)(14) and 609 (15)(16). Another series, with isomeric forms, were identified as esterified catechingallate and glucuronide dimers for ions at m/z 729 (17-18), 745 (19)(20)(21)(22), 761 (23)(24)(25)(26), 785 (27)(28), 881 (29)(30)(31), 897 (32)(33), 913 (34)(35) and 937 (36)(37). The retention time of these isomeric compounds were listed in Table 1.…”

Section: Hplc/esi-it-ms Analysismentioning

confidence: 99%

“…The activities increase proportionally with the raise of the DP [23]. Proanthocyanidins is very important in human health protection and disease prevention; they have been reported to be strong antioxidants [24,25], anticancer [26], anti-ulcerogenic [27], antiprotozoal [28], cicatrizing [29], anti-inflammatory [30] agents and other beneficial properties that can suggest the application of species with tannins in pharmacologic studies, like anti-HIV and anti-HSV effects [31] and pancreatic lipase inhibition properties [32].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Proanthocyanidins from Parkia biglobosa (Jacq.) G. Don. (Fabaceae) by Flow Injection Analysis — Electrospray Ionization Ion Trap Tandem Mass Spectrometry and Liquid Chromatography/Electrospray Ionization Mass Spectrometry

et al. 2013

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Abstract:The present study investigates the chemical composition of the African plant Parkia biglobosa (Fabaceae) roots and barks by Liquid Chromatography -Electrospray Ionization and Direct Injection Tandem Mass Spectrometry analysis. Mass spectral data indicated that B-type oligomers are present, namely procyanidins and prodelphinidins, with their gallate and glucuronide derivatives, some of them in different isomeric forms. The analysis evidenced the presence of up to 40 proanthocyanidins, some of which are reported for the first time. In this study, the antiradical activity of extracts of roots and barks from Parkia biglobosa was evaluated using DPPH method and they showed satisfactory activities.

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“…EGCG has been reported to possess several biochemical and pharmacological properties, which include anti-HIV and HCV infection activity (49,94,318,325), reduction of Sjögren's syndrome in murine models (83,110), prevention of Alzheimer's and Parkinson's diseases (203), anti-obesity effects on mice and humans (41,319), anti-oxidant activity (75,104,293,348), and anti-neoplastic activity (135,262). The cancer-preventive effects of EGCG have been proposed to suppress the transformative, hyperproliferative, and inflammatory processes that are involved in carcinogenesis (288).…”

Section: The Inhibitory Mechanisms Of Natural Compounds Against Npc Smentioning

confidence: 99%

Potential Therapeutic Molecular Targets for Nasopharyngeal Carcinoma

Chen¹

2012

Carcinogenesis, Diagnosis, and Molecular Targeted Treatment for Nasopharyngeal Carcinoma

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Inhibitory effects of (−)-epigallocatechin gallate on the life cycle of human immunodeficiency virus type 1 (HIV-1)

Cited by 201 publications

References 50 publications

A Cell-Based, Microplate Colorimetric Screen Identifies 7,8-Benzoflavone and Green Tea Gallate Catechins as Inhibitors of the Hepatitis C Virus

A Cell-Based, Microplate Colorimetric Screen Identifies 7,8-Benzoflavone and Green Tea Gallate Catechins as Inhibitors of the Hepatitis C Virus

Characterization of Proanthocyanidins from Parkia biglobosa (Jacq.) G. Don. (Fabaceae) by Flow Injection Analysis — Electrospray Ionization Ion Trap Tandem Mass Spectrometry and Liquid Chromatography/Electrospray Ionization Mass Spectrometry

Potential Therapeutic Molecular Targets for Nasopharyngeal Carcinoma

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