Inhibitory effects of estrogenic endocrine disrupting chemicals on fin regeneration in zebrafish are dependent on estrogen receptors

Xia, Chengcai; Tian, Li; Yu, Jie; Lu, Xingfan; Wang, Haixia; He, Zepeng; Qian, Baoliu; Gu, Linqi; Wang, Lina; Lu, Tao; Xu, Chao; Qian, Haifeng; Sun, Liwei

doi:10.1016/j.aquatox.2022.106156

Cited by 3 publications

(1 citation statement)

References 33 publications

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“…Long-term in vivo exposure of adult zebrafish leads to estrogen-impaired spermatogenesis (Assis et al, 2018). 17α-Ethinylestradiol can inhibit caudal fin regeneration in larval zebrafish following fin amputation, which reduces their quality of life and survival in the wild (Xia et al, 2022). In addition, EE contamination of natural aquatic systems induces alterations in locomotor behavior and associated physiological disturbances in wild zebrafish (Goundadkar & Katti, 2017).…”

Section: Introductionmentioning

confidence: 99%

Use of All‐Male cyp17a1‐Deficient Zebrafish (Danio rerio) for Evaluation of Environmental Estrogens

Jia,

Shi,

Liu

et al. 2024

Enviro Toxic and Chemistry

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Natural and synthetic environmental estrogens (EEs) are widespread and have received extensive attention. Our previous studies demonstrated that depletion of the cytochrome P450 17a1 gene (cyp17a1) leads to all‐testis differentiation phenotype in zebrafish and common carp. In the present study, cyp17a1‐deficient zebrafish with defective estrogen biosynthesis were used for the evaluation of EEs, as assessed by monitoring vitellogenin (vtg) expression. A rapid and sensitive assessment procedure was established with the 3‐day administration of estradiol (E2), followed by examination of the transcriptional expression of vtgs in our cyp17a1‐deficient fish. Compared with the control fish, a higher E2‐mediated vtg upregulation observed in cyp17a1‐deficient zebrafish exposed to 0.1 μg/L E2 is known to be estrogen receptor–dependent and likely due to impaired in vivo estrogen biosynthesis. The more responsive vtg expression in cyp17a1‐deficient zebrafish was observed when exposed to 200 and 2000 μg/L bisphenol A (BPA) and perfluoro‐1‐octanesulfonate (PFOS). The estrogenic potentials of E2, BPA, and PFOS were compared and assessed by the feminization effect on ovarian differentiation in cyp17a1‐deficient zebrafish from 18 to 50 days postfertilization, based on which a higher sensitivity of E2 in ovarian differentiation than BPA and PFOS was concluded. Collectively, through the higher sensitivity to EEs and the capacity to distinguish chemicals with different estrogenic potentials exhibited by the all‐male cyp17a1‐deficient zebrafish with impaired estrogen biosynthesis, we demonstrated that they can be used as an excellent in vivo model for the evaluation of EEs. Environ Toxicol Chem 2024;00:1–13. © 2024 SETAC

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Section: Introductionmentioning

confidence: 99%