Inhibitory effects of fermented extract of Ophiopogon japonicas on thrombin-induced vascular smooth muscle cells

Song, Junhui; Jeong, Gi Hee; Park, Sung Lyea; Won, Se Yeon; Paek, Nam Soo; Lee, Bog‐Hieu; Moon, Sung‐Kwon

doi:10.3892/mmr.2015.4499

Cited by 6 publications

(5 citation statements)

References 24 publications

(40 reference statements)

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“…The first study reported that brazilin, a homoisoflavonoid, inhibited PDGF-induced proliferation of rat aortic VSMCs and induced cell-cycle arrest at G 0 /G 1 phase [69]. The other study showed that the homoisoflavonoid-rich plant, Ophiopogon japonicas , exhibited anti-proliferative effects on thrombin-induced rat aortic VSMCs [70]. Similar to brazilin and Ophiopogon japonicas , MP induced the upregulation of p27 [69,70].…”

Section: Discussionmentioning

confidence: 99%

“…The other study showed that the homoisoflavonoid-rich plant, Ophiopogon japonicas , exhibited anti-proliferative effects on thrombin-induced rat aortic VSMCs [70]. Similar to brazilin and Ophiopogon japonicas , MP induced the upregulation of p27 [69,70]. This suggests that homoisoflavonoids attenuate VSMC proliferation by targeting cell cycle regulators.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, MP reduced the invasive capacity of VSMCs by attenuating the expression of the matrix metalloprotease of MMP-2 and MMP-9. Previous studies report that VSMC migration was attenuated by the homoisoflavonoid, brazilin, and the homoisoflavonoid-rich plant, Ophiopogon japonicas [69,70]. However, these studies neither used human arteriolar cells nor assessed the effects on VSMC adhesion or invasion.…”

Section: Discussionmentioning

confidence: 99%

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7-O-Methylpunctatin, a Novel Homoisoflavonoid, Inhibits Phenotypic Switch of Human Arteriolar Smooth Muscle Cells

et al. 2019

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Remodeling of arterioles is a pivotal event in the manifestation of many inflammation-based cardio-vasculopathologies, such as hypertension. During these remodeling events, vascular smooth muscle cells (VSMCs) switch from a contractile to a synthetic phenotype. The latter is characterized by increased proliferation, migration, and invasion. Compounds with anti-inflammatory actions have been successful in attenuating this phenotypic switch. While the vast majority of studies investigating phenotypic modulation were undertaken in VSMCs isolated from large vessels, little is known about the effect of such compounds on phenotypic switch in VSMCs of microvessels (microVSMCs). We have recently characterized a novel homoisoflavonoid that we called 7-O-methylpunctatin (MP). In this study, we show that MP decreased FBS-induced cell proliferation, migration, invasion, and adhesion. MP also attenuated adhesion of THP-1 monocytes to microVSMCs, abolished FBS-induced expression of MMP-2, MMP-9, and NF-κB, as well as reduced activation of ERK1/2 and FAK. Furthermore, MP-treated VSMCs showed an increase in early (myocardin, SM-22α, SM-α) and mid-term (calponin and caldesmon) differentiation markers and a decrease in osteopontin, a protein highly expressed in synthetic VSMCs. MP also reduced transcription of cyclin D1, CDK4 but increased protein levels of p21 and p27. Taken together, these results corroborate an anti-inflammatory action of MP on human microVSMCs. Therefore, by inhibiting the synthetic phenotype of microVSMCs, MP may be a promising modulator for inflammation-induced arteriolar pathophysiology.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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7-O-Methylpunctatin, a Novel Homoisoflavonoid, Inhibits Phenotypic Switch of Human Arteriolar Smooth Muscle Cells

et al. 2019

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“…Plant extracts obtained by fermentation of Rhus verniciflua bark exhibited anticancer activity against the colon cancer cell line HCT-116 and showed the ability to induce apoptosis and inhibit the hedgehog pathway [24]. Dual fermentation of Ophiopogon japonicas extract against Cordyceps militaris, Bifidobacterium longum, Lactobacillus plantarum, and Enterococcus faecium yielded a fermented extract that can prevent cardiovascular disease associated with vascular smooth muscle cell (VSMC) proliferation and migration [25]. Moreover, the fermented plant extract obtained from ginseng in the presence of Aspergillus usamii showed higher anticancer activity against human hepatoma cells (HepG2) and the human colon cancer cell line (DLD-1) compared to the unfermented ginseng extract [22,26].…”

Section: Introductionmentioning

confidence: 99%

Potential Role of Bioactive Compounds: In Vitro Evaluation of the Antioxidant and Antimicrobial Activity of Fermented Milk Thistle

Kucharska,

Grygorcewicz,

Spietelun

et al. 2024

Applied Sciences

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The group of innovative ingredients in cosmetic preparations includes bio-ferments (Bs), which are characterized by high bioactivity and biocompatibility, and one of the plants rich in bioactive compounds that has a beneficial effect on the skin and the body is Silybum marianum. Bio-ferments obtained from this plant are becoming increasingly useful as active ingredients in cosmetics. In the present study, four different bio-ferments were obtained by fermentation of pomace (B-P), extract (B-E), oil (B-O), and seeds (B-S) of milk thistle. Their biodegradability (%B), total polyphenols content (Folin–Ciocalteu method), and antimicrobial, antioxidant (DPPH, ABTS, and FRAP methods), chelating (Fe2+ ions), and reduction (Cu2+ and Fe3+ ions) properties, as well as the acidity, were evaluated. The contact angle using the sessile drop method was assessed to investigate bio-ferments’ impact on skin wettability. Finally, the content of selected phenolic acids in the Bs was evaluated using the HPLC method, while the lactic acid (LA) content was assessed using the GC-MS method. All bio-ferments were characterized by high polyphenols content (13.56 ± 0.10–15.28 ± 0.12 mmol GA/L B), chelating (0.08 ± 0.01–0.17 ± 0.01 mmol Fe2+/L B) and antioxidant activity (DPPH method, 2.41 ± 0.01–3.53 ± 0.01 mmol Tx/L B), and reducing Cu2+ and Fe3+ ions. Gallic acid, protocatechuic acid, caffeic acid, neochlorogenic acid, coumaric acid, and LA were identified in Bs. The most increased antibacterial activity for B-P was observed for a strain of Staphylococcus aureus (MIC = 250 μL/mL) and Pseudomonas aeruginosa (MIC = 250 μL/mL). Simultaneously, B-S demonstrated the highest inhibitory effects against Escherichia coli (MIC = 125 μL/mL), emphasizing the varied antimicrobial profiles of these bio-ferments against different bacterial strains. Research on aerobic biodegradation demonstrated a high level of degradation (%B= 60 ± 1–65 ± 3), and all Bs were categorized as readily degradable according to the OECD classification.

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“…Fermented Rhus verniciflua stem bark extracts showed anticancer activity in a colon cancer cell line HCT-116 and the ability to induce senescence or apoptosis and inhibit the hedgehog pathway [ 11 ]. The fermented Ophiopogon japonicas extract with Cordyceps militaris (first fermentation for 10 days), Lactobacillus plantarum, Enterococcus faecium , and Bifidobacterium longum (second fermentation for 2 days) can prevent cardiovascular diseases associated with the proliferation and migration of vascular smooth muscle cells (VSMCs) [ 12 ]. In turn, fermented Withania somnifera, Emblica officinalis , and Bacopa monnieri extract with Beauveria bassiana ATCC 7159 reduced chick embryo chorioallantoic membrane (CAM) vascularization, suggesting its anti-angiogenic potency [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Biological Activity of Fermented Plant Extracts for Potential Dermal Applications

Herman,

Herman

2023

Pharmaceutics

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Fermented plant extracts (FPEs) are functional liquids formed as a result of the fermentation of fresh plants by microorganisms, mainly bacteria and fungi. The appropriate selection of plants, microorganism strains, and conditions under which the fermentation process is carried out is very important in terms of obtaining a suitable matrix of biologically active compounds with different biological properties. The purpose of this review is to provide verified data on the current knowledge acquired regarding the biological activity of FPEs for cosmetic use and dermal applications. The antioxidant, antimicrobial, anti-inflammatory, anti-melanogenic, and wound-healing activity of FPEs, as well as their potential dermal applications, will be described.

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Inhibitory effects of fermented extract of Ophiopogon japonicas on thrombin-induced vascular smooth muscle cells

Cited by 6 publications

References 24 publications

7-O-Methylpunctatin, a Novel Homoisoflavonoid, Inhibits Phenotypic Switch of Human Arteriolar Smooth Muscle Cells

7-O-Methylpunctatin, a Novel Homoisoflavonoid, Inhibits Phenotypic Switch of Human Arteriolar Smooth Muscle Cells

Potential Role of Bioactive Compounds: In Vitro Evaluation of the Antioxidant and Antimicrobial Activity of Fermented Milk Thistle

Biological Activity of Fermented Plant Extracts for Potential Dermal Applications

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