Inhibitory Effects of Fucoidan on Melanin Synthesis and Tyrosinase Activity

Abstract

Korea -Melanogenesis is a physiological process that results in the synthesis of melanin pigments.Tyrosinase is a key enzyme for melanin biosynthesis, and hyperpigmentation disorders are associated with abnormal accumulation of melanin pigments, which can be improved by treatment with depigmenting agents. Among the possible melanin-reducing compounds, tyrosinase inhibitors are most promising for preventing and treating pigmentation disorder and are used as skin-whitening agents in the cosmetic industry. In the present study, the effects of fucoidan on melanogenesis and tyrosinase activity of B16F10 melanoma cells were investigated. Melanin synthesis and tyrosinase activity in B16F10 melanoma cells were decreased in a dose-dependent manner by fucoidan. Melanin production and tyrosinase activity in B16F10 melanoma cells stimulated by α-melanocyte stimulating hormone (α-MSH) were inhibited by fucoidan with a dose-dependent manner compared to control. Fucoidan inhibited tyrosinase activity of B16F10 melanoma cells with a dose-dependent manner as assessed by 3,4-dihydroxyphenylalanine (DOPA) staining. In conclusion, these findings indicate that fucoidan, which inhibit melanin synthesis and tyrosinase activity, is an effective skin-whitening agent.

Evaluation of Physiological Activity of Traditional Welsh Onion (Allium fistulosum L.)

“…L-tyrosine을 3,4-dihydroxy-phenylalanine (DOPA)로 바꾸는 hydroxylation 반응과 DOPA를 다시 DOPA quinone으로 바꾸는 oxylation 반응에 작용하여 멜라닌을 생성한다 (Hearing & Jiménez, 1987;Jung & Park, 2020). 멜라닌의 생성은 자외선, 건조, 극한 온도 등으로부터 피부를 보호하는 데 도움을 주 지만 과도할 경우 피부 노화를 촉진하고, 기미, 주근깨, 검버섯 등과 같은 색소 침착을 일으킨다 (Jung et al, 2009;Lee et al, 2013) (Sephel & Davidson, 1986). Elastase는 elastin뿐 만 아니라 다른 ECM 단백질과 collagen을 분해하는 효소로 (Tsuji et al, 2001;Lee et al, 2010b), 자외선과 같은 환경적 원인으로 인해 elastase의 활성이 증가하게 되면 elastin의 구조를 끊어 주름을 생성 한다 (Vinson et al, 2001;Tsukahara et al, 2006)…”