Inhibitory Effects of Ginkgo biloba Extract on Inflammatory Mediator Production by Porphyromonas gingivalis Lipopolysaccharide in Murine Macrophages via Nrf-2 Mediated Heme Oxygenase-1 Signaling Pathways

Ryu, Eun Yeon; Park, Ah Jeong; Park, Sun Young; Park, Jong Bae; Eom, Hye Won; Kim, Young Hun; Park, Geuntae; Lee, Sang-Joon

doi:10.1007/s10753-012-9461-6

Cited by 22 publications

(17 citation statements)

References 28 publications

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“…As known, Nrf2 is usually present within the cytosol as a complex with Keap-1 protein and released from this complex and transported into nucleus. Then the transported Nrf2 forms a new complex with Maf protein, and induces the transcription of various antioxidant by binding to antioxidant response element (ARE) on DNA and the specific enzymes that are activated, include HO-1, which may elicit antioxidant and anti-inflammatory effects in many cells [9,[17][18][19][20][21][22]. Meanwhile, HO-1 has been reported to depend on PI3K/p38MAPK signaling pathway in many cells [9,[23][24][25].…”

Section: Discussionmentioning

confidence: 99%

Beta-1-Adrenergic Receptors Mediate Nrf2-HO-1-HMGB1 Axis Regulation to Attenuate Hypoxia/Reoxygenation-Induced Cardiomyocytes Injury in Vitro

Wang

Xie

et al. 2015

Cell Physiol Biochem

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This is an Open Access article licensed under the terms of the Creative Commons AttributionNonCommercial 3.0 Unported license (CC BY-NC) (www.karger.com/OA-license), applicable to the online version of the article only. Distribution permitted for non-commercial purposes only. Key WordsNuclear factor erythroid 2-related factor 2 • Heme oxygenase-1 • High mobility group box 1 protein Abstract Backgroud/Aims: The aim of the study was to evaluate the effects of beta1-adrenergic receptors (β1-ARs) -mediated nuclear factor erythroid 2-related factor 2 (Nrf2)-heme oxygenase-1 (HO-1)-high mobility group box 1 protein (HMGB1) axis regulation in hypoxia/reoxygenation (H/R)-induced neonatal rat cardiomyocytes. Methods: The neonatal cultured cardiomyocytes were concentration-dependently pretreated by dobutamine (DOB), a selective β1-adrenergic receptor agonist, in the absence and/or presence of LY294002 (a phosphatidylinositol 3-kinase (PI3K) inhibitor), SB203580 (a p38mitogen-activated-protein kinase (p38MAPK) inhibitor), Nrf2siRNA and HO-1siRNA, respectively, and then treated by H/R. The effects and mechanisms by which H/R-induced cardiomyocytes injury were evaluated. Results: Significant increase of HO-1 was found in neonatal cultured cardiomyocytes treated with DOB, when compared to the control group. Significant change for Nrf2 translocation was also revealed in neonatal cultured cardiomyocytes treated with DOB. Insignificant decreases of NFkappaB p65 activation and HMGB1 release were observed in H/R-induced neonatal cultured cardiomyocytes treated with DOB, when compared to the control group. Importantly, DOB treatment significantly increased the cell viability and decreased the levels of LDH and MDA in H/R-induced cardiomyocytes injury. However, DOB failed to increase HO-1, inhibit NF-kappaB p65 activation, prevent HMGB1 release and attenuate H/R-induced cardiomyocytes injury when the cultured cardiomyocytes were pretreated by Nrf2siRNA, HO-1siRNA, PI3K inhibitor (LY294002) and p38MAPK inhibitor (SB203580), respectively. Conclusions: β1-ARs-mediated Nrf2-HO-1-HMGB1 axis regulation plays a critical protective role in H/R-induced neonatal rat cardiomyocytes injury in vitro via PI3K/p38MAPK signaling pathway.

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Section: Discussionmentioning

confidence: 99%

Beta-1-Adrenergic Receptors Mediate Nrf2-HO-1-HMGB1 Axis Regulation to Attenuate Hypoxia/Reoxygenation-Induced Cardiomyocytes Injury in Vitro

Wang

Xie

et al. 2015

Cell Physiol Biochem

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“…The LPS from P. gingivalis is a crucial pathogen for the initiation and development of periodontitis by stimulating inflammatory mediator production in periodontal tissues and host immune system [26]. In this study, we demonstrated for the first time that magnolol decreases the release of pro-inflammatory cytokines such as TNF-α and IL-1, and the expression of iNOS and COX-2 accompanied by attenuation of their respective metabolites, nitrite and PGE 2 , formation in macrophages stimulated by LPS from P. gingivalis consistently, treatment with magnolol also significantly decreased the serum levels of proinflammatory cytokines (TNF-α and IL-1β), the myeloperoxidase (MPO) activity, a marker of neutrophil infiltration, and NF-κB activation in lungs of mice challenged by P. gingivalis LPS compared with that of P. gingivalis LPS-injected alone mice (data not show).…”

Section: Discussionmentioning

confidence: 99%

Activation of Nrf2/HO-1signaling pathway involves the anti-inflammatory activity of magnolol in Porphyromonas gingivalis lipopolysaccharide-stimulated mouse RAW 264.7 macrophages

Lü

Hsu

Chen

et al. 2015

International Immunopharmacology

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“…GBE is commonly used to enhance memory and learning (Zhao et al, 2012), as well as, it is one of the most popular herbs that are used to treat amnesia and retard age related memory deficits. GBE may act through several mechanisms including antioxidant effects, inhibition of platelet activating factors, alterations in membrane fluidity (signal transduction), anti‐inflammatory effect (Ryu et al, 2012) and inhibition of glucocorticoid synthesis (Chan et al, 2007). GBE suppresses the over expression of ROS in aluminum‐exposed rats via its antioxidant effect as it has the ability to reduce oxidative stress induced by elevated aluminum brain levels.…”

Section: Discussionmentioning

confidence: 99%

Anti‐amnestic properties of Ginkgo biloba extract on impaired memory function induced by aluminum in rats

Abd‐Elhady

Elsheikh

Khalifa

2013

Intl J of Devlp Neuroscience

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Aluminum is the most widely used non-ferrous metal. However, recently it is reported to be a neurotoxic agent that could induce biochemical defects in brain by affecting levels of neurotransmitters and generating reactive oxygen species resulting in oxidative stress. This study aimed at evaluating neuroprotective effect of Ginkgo biloba extract(2) (GBE) (200 mg/kg for 28 days) in antagonizing aluminum-induced neurotoxicity through investigating certain parameters such as serum aluminum level, brain aluminum content, brain regional distribution of aluminum, brain oxidative stress biomarkers' content, and brain acetylcholinesterase(3) (AChE) activity. Passive avoidance paradigm was used to assess memory retrieval of rats. Rats' activities were studied using open field test. Results showed that administration of aluminum (10 mg/kg for 28 days) impaired rats' memory retrieval associated with marked elevation of aluminum brain content, serum aluminum level and AChE activity. In addition, aluminum treatment induced significant elevation in its brain content in all tested regions. GBE treatment attenuated neurotoxic effects of aluminum as evidenced by improving rats' performance in passive avoidance and lowering brain AChE activity. Moreover, marked elevation in brain content of oxidized glutathione(4) (GSSG) and malonedialdehyde(5) (MDA) as well as depletion of reduced glutathione(6) (GSH) demonstrated following aluminum administration were reversed reaching normal levels after GBE treatment. Open field test, demonstrated no changes in latency period, number of ambulation, rearing, and grooming following aluminum or other treatments. Therefore, GBE may be a promising therapy ameliorating neurotoxicity of aluminum as an environmental toxic agent.

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Inhibitory Effects of Ginkgo biloba Extract on Inflammatory Mediator Production by Porphyromonas gingivalis Lipopolysaccharide in Murine Macrophages via Nrf-2 Mediated Heme Oxygenase-1 Signaling Pathways

Cited by 22 publications

References 28 publications

Beta-1-Adrenergic Receptors Mediate Nrf2-HO-1-HMGB1 Axis Regulation to Attenuate Hypoxia/Reoxygenation-Induced Cardiomyocytes Injury in Vitro

Beta-1-Adrenergic Receptors Mediate Nrf2-HO-1-HMGB1 Axis Regulation to Attenuate Hypoxia/Reoxygenation-Induced Cardiomyocytes Injury in Vitro

Activation of Nrf2/HO-1signaling pathway involves the anti-inflammatory activity of magnolol in Porphyromonas gingivalis lipopolysaccharide-stimulated mouse RAW 264.7 macrophages

Anti‐amnestic properties of Ginkgo biloba extract on impaired memory function induced by aluminum in rats

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