Inhibitory Effects of Glycerol on Growth and Invasion of Human Oral Cancer Cell Lines

Abstract

It has been reported that glycerol enhances susceptibility to radiotherapy and chemotherapy by a chemical chaperone-like effect which restores mutated protein higher-order structures of the p53 protein because p53 functions are recovered to induce p53-dependent apoptosis through radiotherapy and chemotherapy. On the other hand, based on in vitro and in vivo experiments glycerol alone has not been considered to inhibit growth and invasion of cancer cell lines. In the present in vitro study, glycerol alone was added to oral cancer cell lines (tongue cancer, cervical lymph node [CLN] metastasis of tongue cancer, mandibular gingival cancer) and a cisplatin (CDDP) resistant cell line. The results showed that glycerol inhibited the growth and invasive capacities of the cancer cell lines concentration-dependently. Further, it was demonstrated that the mechanisms of glycerol for inhibiting growth and invasion of the cancer cell lines are inhibition of G1/S phase transition of the cancer cell lines, apoptosis induction, and an inability of cancer cells to use glycerol as an energy substrate. Clinically, this may suggest that glycerol has a potential inhibitory effect on the growth and invasion of CDDPresistant oral cancer as well as primary and CLN metastatic lesions of oral cancer.

Metabolic profile and skin-related bioactivities of Cerioporus squamosus hydromethanolic extract

“…Long-chain fatty acids, such as palmitic acid, were testified in many studies to have cytotoxic and antiproliferative activities against cancer cells by inhibiting DNA topoisomerase I and inducing apoptosis in human leukemic cells (Harada et al 2002;Ravi and Krishnan 2017). Glycerol, which is also detected in C. squamosus extract (10.94%), has inhibitory effects on growth and invasion of some human cancer cell lines (Sakurai et al 2011). Interestingly, the majority of remaining compounds detected in the hydromethanolic extract of C. squamosus have also been reported to have anti-cancer properties.…”