Inhibitory Effects of Green Tea Catechins on the Activity of Human Matrix Metalloproteinase 7 (Matrilysin)

Abstract

Inhibitory effects of green tea catechins and their derivatives on the matrilysin-catalyzed hydrolysis of a synthetic substrate, (7-methoxycoumarin-4-yl)acetyl-L-Pro-L-Leu-Gly-L-Leu-[N(3)-(2,4-dinitrophenyl)-L-2,3-diamino-propionyl]-L-Ala-L-Arg-NH(2) [MOCAc-PLGL(Dpa)AR], were examined. The 10 catechins examined were classified into three groups according to their inhibition potency. Catechins with a galloyl group at the 3 position, including a major component of green tea catechin, (-)-epigallo-3-catechin gallate [(-)-EGCG], were the most potent inhibitors and inhibited matrilysin in a non-competitive manner with K(i) values of 0.47-1.65 micro M. The inhibitory potency of (-)-EGCG was not influenced by the presence of an inhibitor, ZnCl(2), suggesting that the inhibitions of matrilysin by (-)-EGCG and by ZnCl(2) might be independent of each other. The inhibitory effects of green tea catechins suggest that a high intake of green tea might be effective for the prevention of tumor metastasis and invasion in which matrilysin is concerned.

Inhibitory effects of chlorogenic acids from green coffee beans and cinnamate derivatives on the activity of porcine pancreas α-amylase isozyme I

“…This suggests that the inhibitory activity of QA seems to increase with increasing the number of CA moieties or the number of hydroxyl groups introduced. This tendency is observed also in the inhibition of MMP-7 by catechins (Oneda, Shiihara, & Inouye, 2003). The QA derivatives substituted with three or more CA moieties or with galloyl groups rather than caffeoyl groups might improve inhibitory activities.…”

Inhibitory Effects of Lignans on the Activity of Human Matrix Metalloproteinase 7 (Matrilysin)

“…The activity (k cat /K m ) increased with increasing [DMSO] in the reaction mixture at high concentrations of 5-hydroxypluviatolide. The dielectric constant of DMSO is 45 (41,45), suggesting that DMSO decreases the hydrophobic interaction and increases the electrostatic interaction in the recognition between the MMP-7 and the lignan. It is …”

Prostate carcinoma and green tea: PSA‐triggered basement membrane degradation and MMP‐2 activation are inhibited by (−)epigallocatechin‐3‐gallate

“…40 In comparison to PMSF, one of the routinely used serine-protease inhibitors (not suitable for human use), EGCG is approximately 20-fold more potent in blocking the PSA-triggered degradation of gelatin. The restraint of PSA-triggered activation of pro-MMP-2, together with the direct inhibition of MMP-2 and other proteases instrumental to cell invasion, [11][12][13][14][15][16][17] thus contributes a possible mechanistic molecular explanation of the PCa risk decline in high green tea consumers very recently registered in a case-control study. 22 In the case of PCa infiltration by inflammatory cells, pro-MMP-2 can be activated also by other proteases released by macrophages and PMNs.…”

“…10 Some vegetable secondary metabolites such as catechins, abundant in green tea, have already been reported to inhibit various metallo-and serine-proteinases instrumental to inflammation, angiogenesis and cancer invasion, 11,12 especially MMP-2, MMP-7, MMP-9 and leukocyte elastase (LE). [13][14][15][16][17] A number of epidemiologic observations have led to the association of green tea consumption with prevention of cancer development and spread, 18 and some studies indicate a chemopreventive effect of green tea on prostate carcinoma (PCa). 19 Epidemiologic surveys register the lowest prostate cancer incidence in Japanese and Chinese populations, consumers of green tea, 20,21 and a very recent case-control study in southeastern China reports that the risk of PCa declines with increasing frequency, duration and quantity of beverage consumption, with an odds ratio (relative to nontea-drinkers) down to 0.13 for those drinking tea for more than 40 years, and 0.09 for those consuming more than 1.5 kg of tea leaf yearly.…”