Inhibitory Effects of Grewia tomentosa Juss. on IgE-Mediated Allergic Reaction and DNCB-Induced Atopic Dermatitis

Abstract

Grewia tomentosa Juss. is a deciduous shrub that mainly grows in Asia. Despite studies of other Grewia species for treatment of various diseases, Grewia tomentosa Juss. has not been studied as a medicinal herb. This study evaluates the anti-allergic and anti-topic dermatitis activity of Grewia tomentosa Juss. ethanol extract (Gt-EE). The results show that Gt-EE suppressed IgE–antigen-induced β-hexosaminidase release. The mRNA expression of IL-1β, IL-4, IL-5, IL-6, IL-13, TNF-α, MCP-1, and TSLP, which are involved in allergic responses, was inhibited by Gt-EE in IgE-stimulated RBL-2H3 cells. In addition, the phosphorylation of Syk, PLCγ1, PKCδ, PI3K, AKT, NF-κB p65, NF-κB p50, p38, JNK, and ERK1/2 was decreased by Gt-EE in these cells. Gt-EE also showed anti-inflammatory effects in in vivo mouse models. In passive cutaneous anaphylaxis (PCA), a commonly used mouse model, Gt-EE decreased the allergic response, infiltration of mast cells, and mRNA level of IL-4. Furthermore, Gt-EE ameliorated symptoms of DNCB-induced atopic dermatitis (AD). In DNCB-induced AD, Gt-EE suppressed the increase in mast cells, serum IgE level, expression of allergic mediators (IL-1β, IL-4, IL-5, IL-6, TNF-α), and phosphorylation of proteins (IκBα, NF-κB p65, NF-κB p50, p38, JNK, and ERK1/2) implicated in allergic reactions

Antiallergic Effects of Callerya atropurpurea Extract In Vitro and in an In Vivo Atopic Dermatitis Model

“…Next, the level of β-hexosaminidase release was tested. IgE-sensitized RBL-2H3 cells, adherent cells with histamine-releasing function [ 37 , 38 ], were incubated with Ca-EE, and HSA stimulated the degranulation of β-hexosaminidase. Ca-EE significantly inhibited β-hexosaminidase release from mast cells at 25–100 μg/mL ( Figure 2 a) without cell cytotoxicity ( Figure 2 b).…”

Anti-Inflammatory Herbal Extracts and Their Drug Discovery Perspective in Atopic Dermatitis

“…Macrophages: Murine macrophage cell line RAW264.7 was used in in vitro models of AD with its stimulator lipopolysaccharide (LPS) ( Ha et al ., 2014 ; Lim et al ., 2014 ; Cho et al ., 2018 ; Kim et al ., 2022 ). Mouse bone marrow-derived macrophages were used for in vitro AD study with LPS stimulation ( Lee et al ., 2022a ). Dendritic cells: Human monocyte-derived dendritic cells were stimulated with a cytokine cocktail (TNF-α/IL-1β/IL-6/PGE2) in an in vitro study of AD ( Chan et al ., 2015 ).…”

“…The ameliorative effect of water root extract (WRE) of P. lactiflora (PL) in an experimental model of AD was recently reported ( Lee et al ., 2022a ). The experimental results indicate that PLWRE has an anti-inflammatory effect in LPS-stimulated bone marrow-derived macrophages by suppressing inflammatory molecules (TNF-α; IL-6, -10, -12; iNOS; and COX-2) and in DNCB-induced AD BALB/c mice by ameliorating serum cytokines (TNF-α, IL-6, and IL-12)/IgE, skin dermatitis, and tissue IL-6/IL-12/IL-17A.…”