Inhibitory effects of Schisandra chinensis extract on acne-related inflammation and UVB-induced photoageing

Abstract

Context: Schisandra chinensis (Turcz.) Baill. (Schisandraceae) fruit extract (SFE) has been reported to induce non-specific tissue protection against inflammation in vivo. However, the effects of SFE on Propionibacterium acnes-stimulated acne and UVB-irradiated photoageing have yet to be investigated. Objective: To systematically investigate the effects of SFE against P. acnes and photoageing in vitro. Materials and methods: Qualitative and quantitative analyses of SFE were performed by HPLC. SFE concentrations from 2.5 to 50 lg/mL were tested. Specifically, ELISA was used to examine the levels of proinflammatory cytokines in THP-1 cells as well as of collagen I and matrix metalloproteinases-1 in HDF cells. The anti-bacterial effect of SFE was determined using the microdilution broth method. Glutathione and malondialdehyde levels were examined using the colorimetric and TBA methods, respectively. The degree of ageing was determined by cytochemical staining. Results: SFE significantly inhibited P. acnes growth (MIC 0.5 mg/mL) and 50 lg/mL of SFE suppressed the production of interleukin-1b, interleukin-8 and tumour necrosis factor a, by 59.67%, 62.69% and 68.30%, respectively, in P. acnes-stimulated THP-1 cells. Additionally, 10 lg/mL of SFE suppressed photoageing in UVB-exposed fibroblasts by decreasing metalloproteinase levels by 88.4%, inducing collagen by 58.4% and activating the anti-oxidant defence system, by limiting lipid peroxidation by 51.1% and increasing glutathione production by 34.1% (2.5 lg/mL SFE). Discussion and conclusion: These results indicated that SFE could significantly ameliorate the inflammatory state in P. acnes-stimulated THP-1 and UVB-irradiated HDF cells, suggesting its potential as a novel agent for acne therapy and photoageing prevention. ARTICLE HISTORY

A Review of the Potential Benefits of Plants Producing Berries in Skin Disorders

“…Pretreatment with the extract exerted positive effects on the antioxidant system, in particular the reduction in the glutathione levels induced by UVB irradiation was reversed by the presence of S. chinensis extract (the effect was statistically significant starting from 5 μg/mL). The MDA levels, compared to UVB-irradiated control group, were significantly decreased following the pretreatment with the extract (10 μg/mL) [ 83 ].…”

Brief Challenges on Medicinal Plants: An Eye‐Opening Look at Ageing‐Related Disorders

“…Schisandrin B, at 10 mmol/L, inhibited NADPH oxidation in mouse liver microsomes incubated with carbon tetrachloride while at 110.0 μmol/L inhibited oxidation of erythrocyte membrane lipids induced by ferric chloride in vitro . On the other hand, the inhibitory effects of schisandra extract on acne‐related inflammation and UV‐B‐irradiated photo ageing revealed that it could dramatically alleviate the inflammatory responses in HDF cells, indicating its promising effects as a potent and efficient agent for acne therapy and photoaging prevention . Also, the highly oxygenated triterpenoid schinchinenlactone D, isolated from roots of schisandra, demonstrated prominent anti‐inflammatory properties .…”

Bioactive Indolyl Diketopiperazines from the Marine Derived Endophytic Aspergillus versicolor DY180635

“…In our continuous investigations of novel bioactive agents from the endophytic fungi [ 23 , 24 ], the endophytic strain Aspergillus versicolor DY180635 isolated from the sea crab was selected based on the bio-evaluation results. The ethyl acetate extracts of a rice solid culture of A. versicolor DY180635 showed 80% inhibition on the anti-inflammation model of the Propionibacterium acnes -induced THP-1 cells at the concentration of 0.1 mg/mL [ 25 ]. High-performance liquid chromatography (HPLC) analysis of the ethyl acetate extracts indicated the presence of IDKPs with a diode array detector (DAD) through ultraviolet characteristics at λ max 236, 289, and 336 nm [ 26 ].…”