Inhibitory effects of luteolin‑4'‑O‑β‑D‑glucopyranoside on P2Y12 and thromboxane�A2 receptor‑mediated amplification of platelet activation in�vitro

Abstract

Platelet activation and subsequent accumulation at sites of vascular injury are central to thrombus formation, which is considered to be a trigger of several cardiovascular diseases. Callicarpa nudiflora (C. nudiflora) Hook is a traditional Chinese medicinal herb for promoting blood circulation by removing blood stasis. In our previous study, several compounds extracted from this herb, including luteolin‑4'‑O‑β‑D‑glucopyranoside (LGP), were revealed to exert inhibitory effects on adenosine diphosphate (ADP)‑induced platelet aggregation. The aim of present study was to confirm these antiplatelet effects and elucidate the potential mechanisms. Using a platelet‑aggregation assay, it was revealed that LGP significantly inhibited platelet aggregation induced by ADP, U46619 and arachidonic acid. It was also found that LGP exhibited marked inhibitory effects on the activation of αIIbβ3 integrin, the secretion of serotonin from granules, and the synthesis of thromboxane A2. In addition, the results showed that LGP suppressed Ras homolog family member A and phosphoinositide 3‑kinase/Akt/glycogen synthase kinase 3β signal transduction. Data from a radiolabeled ligand‑binding assay indicated that LGP exhibited apparent competing effects on thromboxane receptor (TP) and P2Y12 receptors. In conclusion, the data presented here demonstrated that LGP, a natural compound from C. nudiflora Hook, inhibited the development of platelet aggregation and amplification of platelet activation. These inhibitory effects may be associated with its dual‑receptor inhibition on P2Y12 and TP receptors.

Pimpinella pruatjan Molk: LC-MS/MS-QTFT Analysis of Bioactive Compounds from Decoction and Ethanol Extract of Aerial Parts

“…[ 47 ] Luteolin, either aglycone or glycoside, has many biological activities, including: antimicrobial,[ 48 ] antioxidant,[ 49 ] anti-inflammatory activity,[ 50 ] anticancer,[ 51 ] monoamine oxidase inhibition,[ 52 ] inhibitory effects on lens aldose reductase,[ 53 ] and antiplatelet effects. [ 54 ] Luteolin also has multiple cardioprotective effects by enhancing contractility, upregulating autophagy, and preventing cardiac fibrosis[ 55 ] and anti–HIV-1 activity. [ 56 ]…”

Platelet activation: a promoter for psoriasis and its comorbidity, cardiovascular disease

“…Curcumin has also been proven to prevent platelet aggregation in atherosclerosis and lower platelet-leukocyte adhesion in cerebral microcirculation (159). Besides, several other natural products, including Grape seed proanthocyanidin extract, Thymol, Kaempferol, and Luteolin, also alleviate psoriasis via reducing the frequency and function of Th17 cells and increasing that of Treg cells (160), and simultaneously inhibit platelet aggregation, inflammatory cell and platelet adhesion, and thrombogenesis (161)(162)(163)(164). However, research on the efficacy of natural products in psoriasis and psoriasis-associated CVD is still insufficient, awaiting further studies.…”