Inhibitory Effects of Methoxyfenozide on Reproductive Organs of Cotton Leafworm, Spodoptera Littoralis (Boisd.)

Abstract

Current research has focused on the inhibitory effects on the reproductive organs of cotton leafworm, Spodoptera littoralis resulting from 4 th instar larvae treated with sublethal concentration (LC 25) of methoxyfenozide. Results showed elongation in the immature stages and longevity of adults than control, as well as reduce pupal weight, oviposition period, fecundity and hatchability percentages. Anatomical structures of virgin moths surviving from larvae treated with LC 25 of methoxyfenozide showed general atrophy and abnormal features for ovaries and testes than that of untreated ones. Histological disruptions were recorded in ovaries in vitellogenesis and post vitellogensis stages as deformation in follicular shape, clumping of the chromatin material in nurse cells and disorganization of yolk and appearance of vacuoles between yolk and chorion. While in treated testes the testicular wall disappeared and vacuoles between follicles take place. Methoxyfenozide caused alterations in the amounts of both total soluble protein and total lipids and also in the activities of acid and alkaline phosphatases and phenol oxidase in the ovaries and testes of the resulted adults as compared to control adults.

Toxicity, Growth Inhibition Effect, Oxidative Stress Induced in Spodoptera littoralis by Zinc Sulfate and Its Histological Alternation of The Reproductive Organs

“…The female coming from treatments with Azadirchtin and Spinosad showed different morphological abnormalities in ovarioles and oocytes. Sabry et al (2017), studied the inhibitory effects of sublethal concentration (LC25) methoxyfenozide on the reproductive organs of S. littoralis. Results showed elongation in the immature stages in ovaries and longevity of adults than control, as well as reduced pupal weight, oviposition period, fecundity and hatchability percentages.…”

“…Methoxyfenozide caused alterations in the amounts of both total soluble protein and total lipids and also in the activities of acid and alkaline phosphatases and phenol oxidase in the ovaries and testes of the resulting adults as compared to control adults. Sabry et al (2017), suggested that the histopathological effects on the ovaries of S. littoralis females resulted from the effect of the treatment on the plasma membrane osmotic properties which led to dehydration and appearance of vacuoles within the oocytes, shrinkage or degeneration of the yolk and warping of egg chorion vacuolation in the cytoplasm of the nurse cells. Hazaa et al (2009), studied the effect of gamma radiation (125 Gy) on the histological and histochemical structure of the male and female reproductive systems of S. littoralis.…”

Potential of Methoxyfenozide (an Ecdysone Hormone Analogue) on the Growth Duration and Longevity of Adults of Rice Moth, Corcyra cephalonica Staint. (Lepidoptera:Pyralidae) Exposed as First Instar Larvae

“…Adult longevity has also been found to be influenced by the exposure of IGR like methoxyfenozide as observed in case of Spodoptera frugiperda (Zarate et al, 2010); Spodoptera litura (Fabricius) (Rehan and Freed, 2015) and Spodoptera littoralis (Boisd) (Sabry et al, 2017).…”

Bioactivity of Moringa oleifera and Ruta angustifolia Oils on Spodoptera littoralis (Boisd.) Moths’ Vitality

“…Hence, the reproductive organs of female and male insects require a massive maintenance of nutrients and energy resources (Smykal, and Raikhel, 2015). In the same trend (Sabry et al 2017) demonstrated that protein and lipids are known as nutritional indices in insects. Proteins are the fundamental components in both hormones and enzymes.…”

“…Abdelgaleil and El-Sabrout (2018) revealed undifferentiation ovarioles of adults developed from 4 th instar larvae that fed on leaf discs treated with A. monosperma and C. macrocarpa essential oil. Sabry et al (2017) in ovaries and tests of adults derived from treated larvae with LC50 methoxyfenozide treatment may be affecting the plasma membrane osmotic properties, leading to dehydration and appearance of vacuoles within the oocytes ovaries while testes revealed clear reduction in cellular content and bundles of spermatocytes and spermatogonia were severely reduced in number. The tested oils succeed to cause spermatocyte depletion and this play an important role reducing gamete maturing rate and this in finding with Amaldoss (1989) spermatocytes develop into spermatids and sperm bundles are formed as a result of maturation of the spermatids, which completes spermatogenesis.…”