Inhibitory effects of phaseolotoxin on proliferation of leukemia cells HL-60, K-562 and L1210 and pancreatic cells RIN-m5F

Bachmann, André S.; Xu, Ronghui; Ratnapala, L. A.; Patil, Suresh S.

doi:10.1016/j.leukres.2003.07.002

Cited by 5 publications

(6 citation statements)

References 26 publications

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“…We have previously shown that the P. syringae pv. phaseolicola -produced phaseolotoxin inhibits cell proliferation of leukaemic cells and pancreatic cells (Bachmann et al 2004). In this study, we demonstrate for the first time that pure syringolin A inhibits the proliferation of several mammalian cell lines including NB and ovarian cancer cells in a dose-dependent manner and induces apoptosis.…”

Section: Introductionmentioning

confidence: 59%

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Syringolin A, a new plant elicitor from the phytopathogenic bacterium Pseudomonas syringae pv. syringae, inhibits the proliferation of neuroblastoma and ovarian cancer cells and induces apoptosis

Coleman¹,

Rocetes²,

Park³

et al. 2006

Cell Proliferation

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Abstract. Syringolin A is a new plant elicitor produced by the plant pathogen Pseudomonas syringae pv. syringae. The goal of this study was to investigate whether syringolin A exhibits anti‐proliferative properties in cancer cells. The treatment of human neuroblastoma (NB) cells (SK‐N‐SH and LAN‐1) and human ovarian cancer cells (SKOV3) with syringolin A (0–100 µm) inhibited cell proliferation in a dose‐dependent manner. The IC50 (50% inhibition) for each cell line ranged between 20 µm and 25 µm. In SK‐N‐SH cells, the treatment with 20 µm syringolin A led to a rapid (24 h) increase of the apoptosis‐associated tumour suppressor protein p53. In addition, we found that the treatment of SK‐N‐SH cells caused severe morphological changes after 48 h such as rounding of cells and loss of adherence, both conditions observed during apoptosis. The induction of apoptosis by syringolin A was confirmed by both poly (ADP‐ribose) polymerase (PARP) cleavage and annexin V assay. Taken together, we show for the first time that the natural product syringolin A exhibits anti‐proliferative activity and induces apoptosis. Syringolin A and structurally modified syringolin A derivatives may serve as new lead compounds for the development of novel anticancer drugs.

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Section: Introductionmentioning

confidence: 59%

“…We have previously shown that the P. syringae pv. phaseolicola ‐produced phaseolotoxin inhibits cell proliferation of leukaemic cells and pancreatic cells (Bachmann et al. 2004).…”

Section: Introductionmentioning

confidence: 99%

Syringolin A, a new plant elicitor from the phytopathogenic bacterium Pseudomonas syringae pv. syringae, inhibits the proliferation of neuroblastoma and ovarian cancer cells and induces apoptosis

Coleman¹,

Rocetes²,

Park³

et al. 2006

Cell Proliferation

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“…Bachmann's work on polyamines began in 1992 when he studied the role of polyamines during foliar senescence of plant leaves (1). Because of the enthusiasm and encouragements by Dr. Alan Slusarenko and the late Dr. Philippe Matile (University of Zürich, Switzerland), he further delved into the world of polyamines in a quest to identify novel plant ornithine decarboxylase (ODC) 2 inhibitors, using ␣-difluoromethylornithine (DFMO) as a positive control (2)(3)(4). DFMO, also known as eflornithine and Ornidyl, is as a catalytic irreversible (suicide) inhibitor of ODC synthesized in 1978 by researchers at the Merrell-Dow Research Institute (5).…”

mentioning

confidence: 99%

Polyamine synthesis as a target of MYC oncogenes

Bachmann

Geerts

2018

Journal of Biological Chemistry

106

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Edited by Ronald C. Wek This paper is in recognition of the 100th birthday of Dr. Herbert Tabor, a true pioneer in the polyamine field for over 70 years, who served as the editor-in-chief of the Journal of Biological Chemistry from 1971 to 2010. We review current knowledge of MYC proteins (c-MYC, MYCN, and MYCL) and focus on ornithine decarboxylase 1 (ODC1), an important bona fide gene target of MYC, which encodes the sentinel, rate-limiting enzyme in polyamine biosynthesis. Although notable advances have been made in designing inhibitors against the "undruggable" MYCs, their downstream targets and pathways are currently the main avenue for therapeutic anticancer interventions. To this end, the MYC-ODC axis presents an attractive target for managing cancers such as neuroblastoma, a pediatric malignancy in which MYCN gene amplification correlates with poor prognosis and high-risk disease. ODC and polyamine levels are often up-regulated and contribute to tumor hyperproliferation, especially of MYC-driven cancers. We therefore had proposed to repurpose ␣-difluoromethylornithine (DFMO), an FDA-approved, orally available ODC inhibitor, for management of neuroblastoma, and this intervention is now being pursued in several clinical trials. We discuss the regulation of ODC and polyamines, which besides their well-known interactions with DNA and tRNA/rRNA, are involved in regulating RNA transcription and translation, ribosome function, proteasomal degradation, the circadian clock, and immunity, events that are also controlled by MYC proteins.

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“…An improved colorimetric assay for non-adherent cells was introduced [16,17], using the substrate 5-(3-carboxymethoxyphenyl)-2-(4,5-dimethylthiazolyl)3-(4-sulfophenyl) tetrazolium (known as MTS), where the formazan product is soluble in the cell culture medium; however, unreliable results from the MTS assay in the L1210 cell line have been reported [18]. …”

Section: Resultsmentioning

confidence: 99%

Accurate non-invasive image-based cytotoxicity assays for cultured cells

et al. 2010

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BackgroundThe CloneSelect™ Imager system is an image-based visualisation system for cell growth assessment. Traditionally cell proliferation is measured with the colorimetric MTT assay.ResultsHere we show that both the CloneSelect Imager and the MTT approach result in comparable EC50 values when assaying the cytotoxicity of cisplatin and oxaliplatin on various cell lines. However, the image-based technique was found non-invasive, considerably quicker and more accurate than the MTT assay.ConclusionsThis new image-based technique has the potential to replace the cumbersome MTT assay when fast, unbiased and high-throughput cytotoxicity assays are requested.

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Inhibitory effects of phaseolotoxin on proliferation of leukemia cells HL-60, K-562 and L1210 and pancreatic cells RIN-m5F

Cited by 5 publications

References 26 publications

Syringolin A, a new plant elicitor from the phytopathogenic bacterium Pseudomonas syringae pv. syringae, inhibits the proliferation of neuroblastoma and ovarian cancer cells and induces apoptosis

Syringolin A, a new plant elicitor from the phytopathogenic bacterium Pseudomonas syringae pv. syringae, inhibits the proliferation of neuroblastoma and ovarian cancer cells and induces apoptosis

Polyamine synthesis as a target of MYC oncogenes

Accurate non-invasive image-based cytotoxicity assays for cultured cells

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