Inhibitory Effects of Phenolics and Saponins From Commonly Consumed Food Legumes in China Against Digestive Enzymes Pancreatic Lipase andα-Glycosidase

Abstract

Total phenolic and saponin components were extracted from 13 commonly consumed food legumes produced in China, and then a systematic comparative study was conducted to investigate their inhibitory effects against digestive enzymes, pancreatic lipase and α-glycosidase, respectively. Saponin extract (1 mg/mL) from black bean exhibited the highest (41.8%) pancreatic lipase inhibitory effect, followed by phenolic extract from adzuki bean with 36.3%, saponin extract from yellow soybean with 34.1%, saponin extract from pinto bean with 32.6%. It is worth mentioning that both phenolic and saponin extracts from mung bean, adzuki bean, lima Downloaded by [Simon Fraser University] at 04:41 17 March 2015A c c e p t e d M a n u s c r i p t 2 bean, and pinto bean exhibited inhibitory effects against pancreatic lipase. Among four beans, adzuki bean had the highest inhibitory effects against pancreatic lipase activity. In the α-glycosidase assays, phenolic extracts exhibited stronger α-glycosidase inhibitory activity than that of saponins in general. Phenolic extracts of adzuki bean, black bean, fava bean, lentil, rock bean, and red kidney bean inhibited over 80% of α-glycosidase activity at the concentration of 1 mg/mL. Both phenolic and saponin extracts from adzuki bean and rock bean exhibited inhibitory effects against α-glycosidase. The results indicate that adzuki bean is one of the best target beans for further study on their anti-obesity and anti-diabetes effects via cell and animal models.

Profile of phenolic compounds inTrifolium pratenseL. extracts at different growth stages and their biological activities

“…The bioactive compounds have been identified in this plant exhibit health benefits for humans, including antioxidative and antiinflammatory properties. [2,3] These bioactive compounds also occur in a normal diet, in a transformed form, via meat of livestock that feed on clover as well as milk and dairy products. [4] There are several reports on polyphenols with emphasis on isoflavonoid contents.…”

Metabolite profiling, antioxidant activity, and glycosidase inhibition property of the mesocarp tissue extracts of sugar date palm [Phoenix sylvestris(L.) Roxb.] fruits

“…[32] So search for antidiabetic potential of plants and phytochemicals has received attention for the treatment of such disease with many eventual complications. Antidiabetic potential of many edible plants, [33][34][35][36] and grains [37,38] are in report. Fruit extracts and their metabolites are also reported to inhibit α-amylase and α-glucosidase.…”

Antimicrobial and Antioxidant Activities of Extracts and Compounds From Trifolium Baccarinii With Their Mechanisms of Antibacterial Action

“…Some Trifolium species are reported to contain iso avones, avonoids, pterocarpans, saponins, coumarins, and tyramine [10][11][12][13] . The antioxidant, anti-in ammatory and antimicrobial properties of some species of Trifolium were determined [12][13][14][15] . To date, however, no scienti c report could be found in the literature regarding the antioxidant and antimicrobial activities of T. baccarinii, although there is an ample ethnobotanical claim for these properties.…”