Inhibitory Effects of Plant Extracts on Pseudomonas aeruginosa Biofilm Formation

Mashhady, Mohammad Amin; Abkhoo, Javad; Jahani, Somayeh; Abyar, Sima; Khosravani, Forozan

doi:10.17795/iji.38199

Cited by 12 publications

(6 citation statements)

References 17 publications

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“…Results obtained reported that the tested extract had not showed a clearly visible white halo in the violacein bioassay [ 88 ]. It has been demonstrated that a 95% ethanolic extract of T. polium leaves was able to reduce the biofilm formed by P. aeruginosa strain on a polystyrene 96-well plate at 25 ppm [ 89 ]. More recently, a crude extract from air-dried aerial parts of felty germander from Egypt was able to decrease the biofilm formation by S. aureus strains at low concentration [ 90 ].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Screening, Antibacterial, Antifungal, Antiviral, Cytotoxic, and Anti-Quorum-Sensing Properties of Teucrium polium L. Aerial Parts Methanolic Extract

Alreshidi

Noumi

Bouslama

et al. 2020

Plants

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The chemical profile of Teucrium polium L. (T. polium) methanolic extract was tested using liquid chromatography coupled with high resolution mass spectrometry (HR-LCMS). Disc diffusion and microdilution assays were used for the antimicrobial activities. Coxsackievirus B-3 (CVB3) and Herpes simplex virus type 2 (HSV-2) were used for the antiviral activities. Chromobacterium violaceum (ATCC 12472 and CV026) and Pseudomonas aeruginosa PAO1 were used as starter strains for the anti-quorum sensing tests. Isoprenoids are the main class of compounds identified, and 13R-hydroxy-9E,11Z-octadecadienoic acid, valtratum, rhoifolin, sericetin diacetate, and dihydrosamidin were the dominant phytoconstituents. The highest mean diameter of growth inhibition zone was recorded for Acinetobacter baumannii (19.33 ± 1.15 mm). The minimal inhibitory concentrations were ranging from 6.25 to 25 mg/mL for bacterial strains, and from 6.25 to 25 mg/mL for Candida species. The 50% cytotoxic concentration on VERO (African Green Monkey Kidney) cell lines was estimated at 209 µg/mL. No antiviral activity was recorded. Additionally, T. polium extract was able to inhibit P. aeruginosa PAO1 motility in a concentration-dependent manner. However, the tested extract was able to inhibit 23.66% of the swarming and 35.25% of swimming capacities of PAO1 at 100 µg/mL. These results highlighted the role of germander as a potent antimicrobial agent that can interfere with the virulence factors controlled by the quorum-sensing systems.

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Section: Discussionmentioning

confidence: 99%

Phytochemical Screening, Antibacterial, Antifungal, Antiviral, Cytotoxic, and Anti-Quorum-Sensing Properties of Teucrium polium L. Aerial Parts Methanolic Extract

Alreshidi

Noumi

Bouslama

et al. 2020

Plants

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“…Pseudomonas aeruginosa is a Gram-negative bacterium, which can infect plants, animals, and humans [1,2]. In plants, P. aeruginosa can interact with the roots leading to plant death after their colonization via formation of biofilms [3].…”

Section: Introductionmentioning

confidence: 99%

Polyphenols from Salix tetrasperma Impair Virulence and Inhibit Quorum Sensing of Pseudomonas aeruginosa

et al. 2020

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Bacterial resistance represents one of the emerging obstacles in plants, animals, and humans that impairs treatment with antibacterial agents. Targeting of the bacterial quorum sensing system is one of the strategies to overcome this problem. Recently, research has been focused on natural and food components which can function as quorum sensing inhibitors. In this study, a methanol extract from Salix tetrasperma stem bark was phytochemically profiled by LC-MS analysis. This resulted in the identification of 38 secondary metabolites with (epi)catechin-(epi)catechin, epicatechin, tremulacin, salicortin, and trichocarposide as the major constituents. The extracts of both stem bark and the previously profiled flower of S. tetrasperma were tested for anti-quorum sensing activity in a common and widely distributed pathogen Pseudomonas aeruginosa. The natural products inhibited swimming and swarming motilities, as well as proteolytic and hemolytic activities in a dose-dependent manner. Molecular docking of the constituents from both extracts against the quorum sensing controlling systems Lasl/LasR, rhll/rhlR, and PQS/MvfR showed that epicatechin, (epi)catechin-(epi)catechin, p-hydroxy benzoyl galloyl glucose, p-hydroxy benzoyl protocatechuic acid glucose, and caffeoylmalic acid could be the main active components. This study supports the importance of secondary metabolites, especially polyphenols, as quorum sensing inhibitors.

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“…Biofilms are bacterial colonies that have a high tolerance to stress, antimicrobial compounds for a better adaptive habitat [65]. P. aeruginosa is one of the bacterial families that are considered the most sinister pathogen that forms biofilms in the human host [66].…”

Section: Targeting Biological Activities Of P Aeruginosa Through Phyt...mentioning

confidence: 99%

Pseudomonas aeruginosa biofilm and their molecular escape strategies

Avinash¹,

Aishwarya²,

Zameer³

et al. 2023

J App Biol Biotech

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Microbes, the tiny creatures with huge complexity have been the topic of interest in microbiology which has to be explored with a double-edged sword approach. Pseudomonas aeruginosa is the most problematic, opportunistic human bacterial pathogen that has to be addressed with high priority. Understanding their microenvironments at a molecular level becomes important for deciphering the puzzle of their adoptable and virulent behavior in the host cell. The current review attempts in highlighting the secretion systems, especially the Type III secretion system (T3SS) in P. aeruginosa as a potential target to block virulence. This is mainly facilitated through the potent phytochemicals or their formulations (phyto-quoromones) for a synergistic mode of action on T3SS. These phyto-inhibitors will hamper cell signaling through T3SS thus affecting the growth, survival, and replication of the pathogen. The prospects mentioned in the review will provide a newer dimension for the design and development of better and smart therapeutics against Pseudomonas infections.

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Inhibitory Effects of Plant Extracts on Pseudomonas aeruginosa Biofilm Formation

Cited by 12 publications

References 17 publications

Phytochemical Screening, Antibacterial, Antifungal, Antiviral, Cytotoxic, and Anti-Quorum-Sensing Properties of Teucrium polium L. Aerial Parts Methanolic Extract

Phytochemical Screening, Antibacterial, Antifungal, Antiviral, Cytotoxic, and Anti-Quorum-Sensing Properties of Teucrium polium L. Aerial Parts Methanolic Extract

Polyphenols from Salix tetrasperma Impair Virulence and Inhibit Quorum Sensing of Pseudomonas aeruginosa

Pseudomonas aeruginosa biofilm and their molecular escape strategies

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