Inhibitory effects of polyphenol punicalagin on type-II collagen degradation in vitro and inflammation in vivo

Jean-Gilles, Dinorah; Li, Liya; Vaidyanathan, V. G.; King, Roberta S.; Cho, Bongsup; Worthen, David R.; Chichester, C. O.; Seeram, Navindra P.

doi:10.1016/j.cbi.2013.06.018

Cited by 61 publications

(53 citation statements)

References 37 publications

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“…However, the anti-inflammatory properties of pomegranate have not yet been demonstrated in clinical trials (12). Although these studies as well as other studies not mentioned here suggest that pomegranate extract hold good potential as antiinflammatory agent, data on the effects of punicalagin isolated from pomegranate husk is lacking (23,24). The current study has been performed to enlighten the anti-inflammatory properties of punicalagin on LPS-induced RAW 264.7 macrophages.…”

Section: Discussionmentioning

confidence: 80%

“…Treatment of pomegranate extract suppresses the degradation of proteoglycan stimulated by interleukin-1 in human femoral osteoarthritic chondrocytes and synthesis of cellular matrix metalloproteinases. In osteoarthritic chondrocyte cultures it has been shown that pomegranate extract inhibits collagen degradation, and may possibly suppress joint destruction in patients with osteoarthritis (24). However, the anti-inflammatory properties of pomegranate have not yet been demonstrated in clinical trials (12).…”

Section: Discussionmentioning

confidence: 99%

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Punicalagin isolated from Punica granatum husk can decrease the inflammatory response in RAW 264.7 macrophages

Berköz¹,

Allahverdiyev²

2017

Eastern J Med

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Punicalagin isolated from Punica granatum husk can decrease the inflammatory response in RAW 264.7 macrophages

Berköz¹,

Allahverdiyev²

2017

Eastern J Med

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“…Con: control; Mod: treated with LPS (30 µg/ml) only; Low: punicalagin (5 µg/ml)+LPS (30 µg/ml); Mid: punicalagin (10 µg/ml)+LPS (30 µg/ml); High: punicalagin (20 µg/ml)+LPS (30 µg/ml) exhibit anti-inflammatory activity (Jean-Gilles et al, 2013;Olajide et al, 2014;Peng et al, 2015;Yaidikar and Thakur, 2015). Jean-Gilles et al (2013) showed that punicalagin may be toxic to cattle and rats in vivo. In this study, punicalagin did not have toxic effects on bEECs at concentrations of 0-30 µg/ml.…”

Section: Fig 5 Effect Of Punicalagin On Lps-induced Nf-κb Activationmentioning

confidence: 99%

“…Punicalagin exhibits multiple biological effects, including antioxidant, antiproliferative, antiviral, and antimicrobial activities (Taguri et al, 2004;Aqil et al, 2012;Yang et al, 2012;Xu et al, 2016). Moreover, it has shown anti-inflammatory properties both in vitro and in vivo (Jean-Gilles et al, 2013;Olajide et al, 2014;Xu et al, 2014). However, the effects of punicalagin on endometritis have not been investigated.…”

Section: Introductionmentioning

confidence: 99%

Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury

Lyu

Chen

Wang

et al. 2017

J. Zhejiang Univ. Sci. B

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Abstract:Objective: Bovine endometritis is one of the most common reproductive disorders in cattle. The aim of this study was to investigate the anti-inflammation potential of punicalagin in lipopolysaccharide (LPS)-induced bovine endometrial epithelial cells (bEECs) and to uncover the underlying mechanisms. Methods: bEECs were stimulated with different concentrations (1, 10, 30, 50, and 100 μg/ml) of LPS for 3, 6, 9, 12, and 18 h. MTT assay was used to assess cell viability and to identify the conditions for inflammatory injury and effective concentrations of punicalagin. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assess gene expression of pro-inflammatory cytokines. Western blotting was used to assess levels of inflammation-related proteins. Results: Treatment of bEECs with 30 µg/ml LPS for 12 h induced cell injury and reduced cell viability. Punicalagin (5, 10, or 20 µg/ml) pretreatment significantly decreased LPS-induced productions of interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor-α (TNF-α) in bEECs. Molecular research showed that punicalagin inhibited the activation of the upstream mediator nuclear factor-κB (NF-κB) by suppressing the production of inhibitor κBα (IκBα) and phosphorylation of p65. Results also indicated that punicalagin can suppress the phosphorylation of mitogen-activated protein kinases (MAPKs) including p38, c-Jun N-terminal kinase (JNK), and extracellular signal-regulated kinase (ERK). Conclusions: Punicalagin may attenuate LPS-induced inflammatory injury and provide a potential option for the treatment of dairy cows with Escherichia coli endometritis.

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“…No difference in disease scores compared with placebo was uncovered, but high-sensitivity C-reactive protein was significantly lowered and this was associated with decreased score (90) . Pomegranate juice or extracts, which have been reported to contain anthocyanins and many other flavonoids including flavanols, have been shown to inhibit IL-1-induced MMP expression in chondrocytes via inhibition of MAP kinases and NF-κB (91)(92)(93) . Such extracts also show efficacy in the monosodium iodoacetate model of OA in mice (94) .…”

Section: Anthocyaninsmentioning

confidence: 99%

The potential for dietary factors to prevent or treat osteoarthritis

et al. 2014

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Osteoarthritis (OA) is a degenerative joint disease for which there are no disease-modifying drugs. It is a leading cause of disability in the UK. Increasing age and obesity are both major risk factors for OA and the health and economic burden of this disease will increase in the future. Focusing on compounds from the habitual diet that may prevent the onset or slow the progression of OA is a strategy that has been under-investigated to date. An approach that relies on dietary modification is clearly attractive in terms of risk/benefit and more likely to be implementable at the population level. However, before undertaking a full clinical trial to examine potential efficacy, detailed molecular studies are required in order to optimise the design. This review focuses on potential dietary factors that may reduce the risk or progression of OA, including micronutrients, fatty acids, flavonoids and other phytochemicals. It therefore ignores data coming from classical inflammatory arthritides and nutraceuticals such as glucosamine and chondroitin. In conclusion, diet offers a route by which the health of the joint can be protected and OA incidence or progression decreased. In a chronic disease, with risk factors increasing in the population and with no pharmaceutical cure, an understanding of this will be crucial.Osteoarthritis: Diet: Cartilage: Bioactive: Polyphenol: Phytochemical: Flavonoid Osteoarthritis (OA) is a degenerative joint disease characterised by degradation of articular cartilage, thickening of subchondral bone and osteophyte formation. Incidence and prevalence of OA has been difficult to assess, in part because of heterogeneity in definitions of the disease. A recent meta-analysis suggested that overall prevalence of OA at different anatomical sites was 23·9 % (knee), 10·9 % (hip) and 43·3 % (hand), although only the prevalence of knee OA showed a gender difference between women and men (27·3 and 21 %, respectively)(1) . Osteoarthritis is a leading cause of disability in the UK. A recent survey (2) found 8·5 million people in the UK with OA, with 71 % of these in constant pain. There are no effective disease-modifying drugs to treat OA and drugs that relieve pain are often insufficient. Joint replacement is offered to patients at end-stage disease with 66 436 hip and 77 578 knee replacements due to OA performed in the UK in 2011 (3) . Two major risk factors for OA are increasing age (most affected patients are aged >45 years and the greatest morbidity is seen in patients aged >60 years) (4) and increasing obesity (5) . With changing demographics, OA is an increasing public health and economic burden. The economic costs of OA in the UK are largely unknown, but direct costs have been estimated at approximately £1 billion/year. With inclusion of indirect costs, estimates from the USA range up to £8 billion/ year (6) .*Corresponding author: I. M. Clark, fax 01603-592250, email i.clark@uea.ac.uk †These authors contributed equally to this review.Abbreviations: ADAMTS, a disintegrin and metalloproteina...

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Inhibitory effects of polyphenol punicalagin on type-II collagen degradation in vitro and inflammation in vivo

Cited by 61 publications

References 37 publications

Punicalagin isolated from Punica granatum husk can decrease the inflammatory response in RAW 264.7 macrophages

Punicalagin isolated from Punica granatum husk can decrease the inflammatory response in RAW 264.7 macrophages

Punicalagin protects bovine endometrial epithelial cells against lipopolysaccharide-induced inflammatory injury

The potential for dietary factors to prevent or treat osteoarthritis

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