Inhibitory effects of salidroside and paeonol on tyrosinase activity and melanin synthesis in mouse B16F10 melanoma cells and ultraviolet B-induced pigmentation in guinea pig skin

Peng, Li‐Hua; Liu, Shuai; Xu, Shen-Yao; Chen, Lei; Shan, Ying-Hui; Wei, Wei; Liang, Wenquan; J, Gao

doi:10.1016/j.phymed.2013.04.015

Cited by 78 publications

(54 citation statements)

References 41 publications

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“…이미 많은 연구에서 resveratrol의 항 멜라닌 생성에 대한 효과를 입증하였으며 [21], 특히 tyrosinase-related protein (TPR-1, TPR-2) 그리고 microphthalmiaassociated transcription factor(MITF)에 관여하는 tyrosinase 활 성을 강하게 억제하는 것으로 보고되었다 [22]. 표피의 과색 소 침착은 과도한 멜라닌 합성에 의해 발생하며 tyrosinase는 멜라닌 생성의 결정적인 역할을 하는 효소로써 L-DOPA에 의해 멜라닌을 합성한다 [23]. 따라서 티로시나제 억제제는 피부 색소침착을 미연에 예방할 수 있는 피부 미백화장품 소 재로써 사용이 가능하다.…”

Section: Raw 2647 세포에서 Reactive Oxygen Species (Ros)unclassified

A Study on Peanut Spouts Extract as the Anti-oxidant Activity and the Skin Whitening Cosmetic Ingredients

윤미연

2016

KSBB Journal

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To investigate the effect of peanut sprout extract on skin care, we measured anti-oxidant activity and whitening action. As a result of measuring DPPH radical scavenging activity to examine independent anti-oxidation of peanut sprout extract, there was strongly scavenging activity. Fluorescent material DCF-DA was used to measure hydrogen peroxide created in RAW 264.7 cells, and all concentration dependently decreased ROS production. As a result of measuring nitric oxide to examine anti-inflammation of peanut sprout extract, there was strongly inhibited nitric oxide production in RAW 264.7 cells. Tyrosinase activation was found to inhibited dose-dependant. Melanin production was also prevented dose-dependant. Therefore, it is expected to be used effectively in development of functional cosmetic materials.

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Section: Raw 2647 세포에서 Reactive Oxygen Species (Ros)unclassified

A Study on Peanut Spouts Extract as the Anti-oxidant Activity and the Skin Whitening Cosmetic Ingredients

윤미연

2016

KSBB Journal

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“…In a previous study, we reported on two natural products, ie, paeonol and salidroside, which have anti-melanogenesis effects by inhibition of tyrosinase activity and melanocyte proliferation, respectively. 18 Given that salidroside and paeonol can interfere with synthesis of melanin via several mechanisms, it is widely hypothesized that combined delivery of these compounds would be very useful for enhancing their therapeutic efficacy and would allow the drug doses to be decreased, thereby reducing their potential toxic effects. However, as far as we know, the evidence for a synergistic effect of multiple drugs in anti-melanogenesis is scarce.…”

Section: Introductionmentioning

confidence: 99%

Sequential release of salidroside and paeonol from a nanosphere-hydrogel system inhibits ultraviolet B-induced melanogenesis in guinea pig skin

Peng¹,

Xu²,

Shan³

et al. 2014

IJN

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Melanin is the one of most important pigments for skin color in mammals. Excessive biosynthesis of melanin induces various pigment disorders. Much effort has been made to develop regulators to minimize skin pigmentation abnormalities. However, only a few of them are used, primarily because of safety concerns and low efficiency. In this study, we aimed to construct a novel nanosphere-gel for sequential delivery of salidroside and paeonol, to investigate the synergistic effects of these drugs in anti-melanogenesis, and to decrease their potential for toxicity in high dosage. Nanospheres were prepared and characterized for their particle size, polydispersity index, zeta potential, and morphological properties. The optimized nanospheres were incorporated in carbomer hydrogel with both paeonol and salidroside entrapped to form a dual drug-releasing nanosphere-gel. With this nanosphere-gel, rapid release of salidroside from the hydrogel followed by sustained release of paeonol from the nanosphere was achieved. Using a classical model of the melanogenesis response to ultraviolet exposure, it was shown that the anti-melanogenesis effects of the dual drug-releasing system, in which the doses of the individual drugs were decreased by half, was obviously enhanced when compared with the effects of the single drug preparations. Mechanistically, the burst release of salidroside from the hydrogel may enable prompt suppression of melanocyte proliferation on exposure to ultraviolet B radiation, while the paeonol released in a sustained manner can provide continuous inhibition of tyrosinase activity in melanocytes. Combined delivery of salidroside and paeonol was demonstrated to be a promising strategy for enhancing the therapeutic efficacy of these agents in anti-melanogenesis and reducing their toxicity, so may have great potential in nanomedicine.

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“…In this previous report, resveratrol strongly down-regulated tyrosinase, tyrosinase-related protein (TPR)-1, TPR-2, and microphthalmia-associated transcription factor (MITF) in animal study . Tyrosinase plays an important role in determining melanin production (Peng et al, 2013) by initiating melanin synthesis through the conversion of tyrosine to 3,4-dihydroxyphenylalanine (L-DOPA). TRP-1 and TPR-2 stabilize tyrosinase and regulate its function in melanin formation; these three types of enzymes are key mediators of melanogenesis that is promoted by the binding of MITF (Newton …”

Section: Introductionmentioning

confidence: 99%

Resveratrol-Enriched Rice Down-Regulates Melanin Synthesis in UVB-Induced Guinea Pigs Epidermal Skin Tissue

Lee¹,

Seo²,

Ho³

et al. 2014

Biomol Ther

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. www.biomolther.orgSynthetic compounds that are used in the clinic to regulate skin hyperpigmentation, such as arbutin, hydroquinone, and kojic acid, are only moderately effective. But, their use is limited by side effects. As part of an effort to overcome the limitations, we developed resveratrol-enriched rice (RR) using genetic engineering technique. Each of resveratrol and rice has been reported to produce anti-melanogenic effects. Therefore, we hypothesized that RR would show more anti-melanogenic effects than those of resveratrol or rice alone. Anti-melanogenic effect of RR was done by using melan-a mouse melanocytes. The depigmenting efficacy was then observed following topical application of the RR to UVB-stimulated hyperpigmented dorsal skin of guinea pigs. Treatment with RR extract resulted a 21.4 ± 0.7% decrease in tyrosinase expression at melan-a cells. Colorimetric analysis showed a significantly lower depigmenting value by day 9 following treatment with RR in UVB-irradiated guinea pigs the dorsal skin (p<0.01), indicating that RR produced a depigmentation effect. By staining with Fontana-Masson stain, we found that the RR-treated group had more effect histopathologically in epidermal melanin production than resveratrol or rice alone-treated group. RR was associated with reduction in the levels of microphthalmia-associated transcription factor (MITF), and downregulation of tyrosinase and tyrosinaserelated protein (TRP-2) expression, leading to inhibit epidermal melanin production by western blot analysis. This study suggests that the resveratrol-enriched rice may be a promising candidate in regulating skin pigmentation with UVB exposure.

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Inhibitory effects of salidroside and paeonol on tyrosinase activity and melanin synthesis in mouse B16F10 melanoma cells and ultraviolet B-induced pigmentation in guinea pig skin

Cited by 78 publications

References 41 publications

A Study on Peanut Spouts Extract as the Anti-oxidant Activity and the Skin Whitening Cosmetic Ingredients

A Study on Peanut Spouts Extract as the Anti-oxidant Activity and the Skin Whitening Cosmetic Ingredients

Sequential release of salidroside and paeonol from a nanosphere-hydrogel system inhibits ultraviolet B-induced melanogenesis in guinea pig skin

Resveratrol-Enriched Rice Down-Regulates Melanin Synthesis in UVB-Induced Guinea Pigs Epidermal Skin Tissue

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