Inhibitory effects of sanguinarine on monoamine oxidase activity in mouse brain

Abstract

The effects of benzophenanthridine alkaloids, such as sanguinarine and chelidonine, on monoamine -oxidase (MAO) activity in mouse brain were investigated. Sanguinarine showed an inhibitory effect on MAO activity in a concentration dependent manner (53.4% inhibition at 25 microM). However, chelidonine did not inhibit MAO activity. The IC(50) value of sanguinarine was 24.5 microM. Sanguinarine inhibited non-competitively MAO activity using kynuramine as a substrate. The K(i) value for sanguinarine was 22.1 microM. These results suggest that sanguinarine partially contributes to the regulation of catecholamine content.

Inhibitory Activity on Binding of Specific Ligands to the Human Angiotensin II AT1and Endothelin 1 ETAReceptors: Bioactive Benzo[c]phenanthridine Alkaloids from the Root ofBocconia frutescens

“…Apparently, at physiological pH the iminium ion form at position 5 of the quaternary benzo skeleton becomes an alkanolamine form [24], which increases lipophilicity. The planarity of the sanguinarine molecule has shown more affinity for enzymes such as monoamine oxidase, when compared to chelidonine [25]. Interestingly, sanguinarine showed more affinity for the AT 1 receptor than chelidonine.…”

Amitozyn Impairs Chromosome Segregation and Induces Apoptosis via Mitotic Checkpoint Activation

“…In addition, they exert a dose-dependent inhibition of angiotensin- and endothelin receptors [11] and inhibit the activity of some enzymes, such as lipoxygenases and aromatic amino acid decarboxylases [12], [13]. Sanguinarine has been shown to perturb microtubule assembly [14] and inhibit the activity of some enzymes [15], [16], while the mechanism of chelerythrine activity is not clear. It was proposed to be a potent inhibitor of protein kinase C [17], but this has later been questioned [18].…”

Sanguinarine

“…Molecular biological studies indicate that SA may affect diverse cellular/molecular targets some of which may directly impact on the cardiovascular system. For example, it may perturb microtubule assembly (Lopus and Panda 2006; Wolff and Knipling 1993); and affect membrane permeability (Babich et al 1996; Schmeller et al 1997); and inhibit a wide variety of enzymes such as acetylcholine esterase, butyrylcholinesterase, choline acetyl transferase, (Schmeller et al 1997) protein kinases, (Wang et al 1997) and even monoamine oxidase (Lee et al 2001). SA has also been reported to suppress angiogenesis (Eun and Koh 2004).…”