Inhibitory effects of sodium new houttuyfonate on growth and biofilm formation of Streptococcus mutans

Shui, Yusen; Jiang, Qingsong; Lyu, Xiaoying; Wang, Liang; Lin, Yi‐Dan; Ma, Qizhao; Gong, Tao; Zeng, Jumei; Yang, Ran; Li, Yuqing

doi:10.1016/j.micpath.2021.104957

Cited by 11 publications

(14 citation statements)

References 39 publications

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“…The results are presented as the mean ± SD of three biological replicates. In addition, the SMS and SMN samples obtained from the cultivation after 14 days in HARV bioreactors were diluted to 10 7 CFU/ml and immediately subjected to CFU assay to identify the differences in viability (Shui et al, 2021).…”

Section: Quantitative Analysis Of the Biofilm Formation Abilitymentioning

confidence: 99%

“…After removing the stain and washing two times with sterile water, images were acquired from random positions, and the number of images from each layer varied according to the thickness of the biofilm. The maximum biofilm thickness of the uniform position in the current field of view was set as the biofilm thickness (Jiang et al, 2021;Shui et al, 2021).…”

Section: Analysis Of the Biofilm Formation Ability Via Confocal Laser-scanning Microscopymentioning

confidence: 99%

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Effects of Simulated Microgravity on the Physiology of Stenotrophomonas maltophilia and Multiomic Analysis

Guo

Fang

et al. 2021

Front. Microbiol.

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Many studies have shown that the space environment plays a pivotal role in changing the characteristics of conditional pathogens, especially their pathogenicity and virulence. However, Stenotrophomonas maltophilia, a type of conditional pathogen that has shown to a gradual increase in clinical morbidity in recent years, has rarely been reported for its impact in space. In this study, S. maltophilia was exposed to a simulated microgravity (SMG) environment in high-aspect ratio rotating-wall vessel bioreactors for 14days, while the control group was exposed to the same bioreactors in a normal gravity (NG) environment. Then, combined phenotypic, genomic, transcriptomic, and proteomic analyses were conducted to compare the influence of the SMG and NG on S. maltophilia. The results showed that S. maltophilia in simulated microgravity displayed an increased growth rate, enhanced biofilm formation ability, increased swimming motility, and metabolic alterations compared with those of S. maltophilia in normal gravity and the original strain of S. maltophilia. Clusters of Orthologous Groups (COG) annotation analysis indicated that the increased growth rate might be related to the upregulation of differentially expressed genes (DEGs) involved in energy metabolism and conversion, secondary metabolite biosynthesis, transport and catabolism, intracellular trafficking, secretion, and vesicular transport. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that the increased motility might be associated the upregulation of differentially expressed proteins (DEPs) involved in locomotion, localization, biological adhesion, and binding, in accordance with the upregulated DEGs in cell motility according to COG classification, including pilP, pilM, flgE, flgG, and ronN. Additionally, the increased biofilm formation ability might be associated with the upregulation of DEPs involved in biofilm formation, the bacterial secretion system, biological adhesion, and cell adhesion, which were shown to be regulated by the differentially expressed genes (chpB, chpC, rpoN, pilA, pilG, pilH, and pilJ) through the integration of transcriptomic and proteomic analyses. These results suggested that simulated microgravity might increase the level of corresponding functional proteins by upregulating related genes to alter physiological characteristics and modulate growth rate, motility, biofilm formation, and metabolism. In conclusion, this study is the first general analysis of the phenotypic, genomic, transcriptomic, and proteomic changes in S. maltophilia under simulated microgravity and provides some suggestions for future studies of space microbiology.

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Section: Quantitative Analysis Of the Biofilm Formation Abilitymentioning

confidence: 99%

Section: Analysis Of the Biofilm Formation Ability Via Confocal Laser-scanning Microscopymentioning

confidence: 99%

Effects of Simulated Microgravity on the Physiology of Stenotrophomonas maltophilia and Multiomic Analysis

Guo

Fang

et al. 2021

Front. Microbiol.

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“…Sodium new houttuyfonate (SNH), a sodium bisulfite of houttuynia isolated from Houttuynia cordata, also showed an excellent ability to reduce EPS production. GtfBC of S. mutans was downregulated after the SNH treatment at 100 μg/ ml (1/2 MIC), and GtfB expression was even downregulated by >50% (Shui et al, 2021). Curcumin treatment for 5 min was also downregulated the Ftf gene expression of S. mutans by 0.541-fold (Li et al, 2020b).…”

Section: Reduction In Eps Generationmentioning

confidence: 96%

“…The XIP interacts with and activates ComR to regulate the expression of ComX (Wenderska et al, 2017). Rhodiola rosea downregulated the ComDE gene expression at 0.25 mg/ml (Zhang et al, 2020a), while the gene expression of ComD was suppressed by >50% under SNH treatment at 100 μg/ ml (Shui et al, 2021). Baicalin was able to inhibit ComX gene expression at 500 μg/ml (Elango et al, 2021).…”

Section: Inhibition Of the Qs Systemmentioning

confidence: 99%

“…In dental caries, many natural products, such as propolis and sodium new houttuyfonate, were able to inhibit S. mutans biofilm virulence, EPS production and QS system (Veloz et al, 2016;Shui et al, 2021). Propolis inhibited S. mutans biofilm formation and dental caries development in a rat model (Duarte et al, 2006), while magnolol and honokiol extracted from magnolia bark reduced the biofilm formation by an in vivo Germ-kill model (Greenberg et al, 2007).…”

Section: Natural Products As An Alternative Treatment For Oral Infectionmentioning

confidence: 99%

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Natural products from traditional medicine as promising agents targeting at different stages of oral biofilm development

Chi

Wang

et al. 2022

Front. Microbiol.

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Oral cavity is an ideal habitat for more than 1,000 species of microorganisms. The diverse oral microbes form biofilms over the hard and soft tissues in the oral cavity, affecting the oral ecological balance and the development of oral diseases, such as caries, apical periodontitis, and periodontitis. Currently, antibiotics are the primary agents against infectious diseases; however, the emergence of drug resistance and the disruption of oral microecology have challenged their applications. The discovery of new antibiotic-independent agents is a promising strategy against biofilm-induced infections. Natural products from traditional medicine have shown potential antibiofilm activities in the oral cavity with high safety, cost-effectiveness, and minimal adverse drug reactions. Aiming to highlight the importance and functions of natural products from traditional medicine against oral biofilms, here we summarized and discussed the antibiofilm effects of natural products targeting at different stages of the biofilm formation process, including adhesion, proliferation, maturation, and dispersion, and their effects on multi-species biofilms. The perspective of antibiofilm agents for oral infectious diseases to restore the balance of oral microecology is also discussed.

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Comparing proteome changes involved in biofilm formation byStreptococcus mutansafter exposure to sucrose and starch

Rezaei

Kamounah

Khodadadi

et al. 2023

Biotech and App Biochem

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Streptococcus mutans is a main organism of tooth infections including tooth decay and periodontitis. The aim of this study was to assess the influence of sucrose and starch on biofilm formation and proteome profile of S. mutans ATCC 35668 strain. The biofilm formation was assessed by microtiter plating method. Changes in bacterial proteins after exposure to sucrose and starch carbohydrates were analyzed using matrix‐assisted laser desorption/ionization mass spectrometry. The biofilm formation of S. mutans was increased to 391.76% in 1% sucrose concentration, 165.76% in 1% starch, and 264.27% in the 0.5% sucrose plus 0.5% starch in comparison to biofilm formation in the media without sugars. The abundance of glutamines, adenylate kinase, and 50S ribosomal protein L29 was increased under exposure to sucrose. Upregulation of lactate utilization protein C, 5‐hydroxybenzimidazole synthase BzaA, and 50S ribosomal protein L16 was formed under starch exposure. Ribosome‐recycling factor, peptide chain release factor 1, and peptide methionine sulfoxide reductase MsrB were upregulated under exposure to sucrose in combination with starch. The results demonstrated that the carbohydrates increase microbial pathogenicity. In addition, sucrose and starch carbohydrates can induce biofilm formation of S. mutans via various mechanisms such as changes in the expression of special proteins.

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Inhibitory effects of sodium new houttuyfonate on growth and biofilm formation of Streptococcus mutans

Cited by 11 publications

References 39 publications

Effects of Simulated Microgravity on the Physiology of Stenotrophomonas maltophilia and Multiomic Analysis

Effects of Simulated Microgravity on the Physiology of Stenotrophomonas maltophilia and Multiomic Analysis

Natural products from traditional medicine as promising agents targeting at different stages of oral biofilm development

Comparing proteome changes involved in biofilm formation byStreptococcus mutansafter exposure to sucrose and starch

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