Inhibitory effects of the root extract of Litsea cubeba (lour.) pers. on adjuvant arthritis in rats

Lin, Bangjiang; Zhang, Hong; Zhao, Xiangxiang; Rahman, Khalid; Wang, Ying; Ma, Xueqin; Zheng, Cheng‐Jian; Zhang, Qiaoyan; Han, Ting; Qin, Lu‐Ping

doi:10.1016/j.jep.2013.03.011

Cited by 75 publications

(55 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many pharmacological activities of L. cubeba extract and its compounds have been reported such as anticancer, antibacterial, antioxidant and antiinflammatory immunological activities. Our previous study also proved that L. cubeba exhibited remarkable anti-arthritic activity on Freund's adjuvant induced arthritis in rats (Lin et al 2013). So far, the anti-arthritic active constituent has not been discovered yet.…”

Section: Introductionmentioning

confidence: 82%

“…However, the use of these drugs always associated with long-term side-effects, toxicity, high cost and incurs hypersensitivity to medications and infections (Feldmann et al 2001). In fact, herbal medicine is being widely used virtually around the world for the treatment of rheumatic and arthritic diseases, in recent decades, considerable advances have been made in both clinical and basic research on the treatment of RA (Soeken et al 2003;Lin et al 2013).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Anti-inflammatory constituents from the root of Litsea cubeba in LPS-induced RAW 264.7 macrophages

Lin

Sun

Xin

et al. 2016

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

Context Litsea cubeba (Lour.) Pers. (Lauraceae) has long been used as a folk remedy in Traditional Chinese Medicine (TCM) for the treatment of rheumatic diseases. Previous studies from our laboratory indicated that L. cubeba extract showed anti-arthritic activity in rats. Objective To study L. cubeba chemically and biologically and to find the potential constituents responsible for its anti-arthritic effect. Materials and methods The compounds were isolated from the root of L. cubeba by column chromatography which eluted with PE:EtOAc gradient system, and the structures were elucidated by detailed spectroscopic data analysis; the anti-inflammatory activity of the isolated compounds was evaluated by lipopolysaccharide (LPS)-induced RAW 264.7 cells and the TNF-a and NO level were measured by ELISA (commercial kit); The iNOS and COX-2 mRNA expression were measured by RT-PCR and the phosphorylation of IkBa, IKKb, P38 and Akt were determined by western blots. Results A novel 9-fluorenone, 1-ethoxy-3,7-dihydroxy-4,6-dimethoxy-9-fluorenone (1), together with 4 known compounds, namely pinoresinol (2), syringaresinol (3), 9,90 -dimethoxysecoisolariciresinol (4) and lyoniresinol (5) were isolated from the root of L. cubeba for the first time. The IC 50 for NO inhibition on compounds 1 and 4 were 56.1 ± 1.2 and 32.8 ± 2.3 mM, respectively. The IC 50 for TNF-a inhibition were 28.2 ± 0.9 and 15.0 ± 1.0 mM, respectively. Both 1 and 4 suppress mRNA expression of iNOS, COX-2 and protein phosphorylation of IkBa, IKKb in LPS-induced RAW 264.7 cells. Discussion and conclusion Compounds 1 and 4 isolated from L. cubeba exhibited potent antiinflammatory activity through the NF-kB signal pathway. ARTICLE HISTORY

show abstract

Section: Introductionmentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory constituents from the root of Litsea cubeba in LPS-induced RAW 264.7 macrophages

Lin

Sun

Xin

et al. 2016

Pharmaceutical Biology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Furthermore, IL-10 is known to be an immunomodulatory and anti-inflammatory cytokine [6,[42][43][44][45].…”

Section: Discussionmentioning

confidence: 99%

Pristimerin, a naturally occurring triterpenoid, protects against autoimmune arthritis by modulating the cellular and soluble immune mediators of inflammation and tissue damage

Nanjundaiah

Venkatesha

et al. 2014

Clinical Immunology

View full text Add to dashboard Cite

Rheumatoid arthritis (RA) is a chronic autoimmune disorder affecting the synovial joints. The currently available drugs for RA are effective only in a proportion of patients and their prolonged use is associated with severe adverse effects. Thus, new anti-arthritic agents are being sought. We tested Pristimerin, a naturally occurring triterpenoid, for its therapeutic activity against rat adjuvant arthritis. Pristimerin effectively inhibited both arthritic inflammation and cartilage and bone damage in the joints. Pristimerin-treated rats exhibited a reduction in the pro-inflammatory cytokines (IL-6, IL-17, IL-18, and IL-23) and the IL-6/IL-17-associated transcription factors (pSTAT3 and ROR-γt), coupled with an increase in the immunomodulatory cytokine IL-10. Also increased was IFN-γ, which can inhibit IL-17 response. In addition, the Th17/Treg ratio was altered in favor of immune suppression and the RANKL/OPG ratio was skewed towards anti-osteoclastogenesis. This is the first report on testing Pristimerin in arthritis. We suggest further evaluation of Pristimerin in RA patients.

show abstract

“…During the course of the treatment, the body weight of each rat was measured with a 0.1 g precision balance (METTLLER TOLDEO, PB1501) every 3 days, and the change of body weight was expressed as weight growth (g) [20]. …”

Section: Methodsmentioning

confidence: 99%

“…The thymus and spleen were immediately removed from each animal and weighed. The index of thymus and spleen was expressed as the ratio (mg/g) of thymus and spleen wet weight versus body weight, respectively [20]. After the tissues were gently collected from the rats, their bodies were kept intact and clean as far as possible and buried under a specific tomb.…”

Section: Methodsmentioning

confidence: 99%

Therapeutic effects of total steroid saponin extracts from the rhizome of Dioscorea zingiberensis C.H.Wright in Freund's complete adjuvant induced arthritis in rats

Zhang¹,

Ito²,

Liang³

et al. 2014

International Immunopharmacology

View full text Add to dashboard Cite

The aim of our present study is to explore the anti-arthritic potential effect of total steroid saponins (TSSN) extracted from the rhizome of Dioscorea zingiberensis C.H.Wright (DZW) and to investigate the underlying mechanisms. This work was performed using adjuvant-induced arthritis (AIA) rats in vivo and lipopolysaccharide (LPS) simulated 264.7 macrophage cells in vitro. In AIA-induced arthritic rats, TSSN significantly alleviated the arthritic progression through evaluating arthritic score, immune organ indexes, paw swelling, and body weight. This phenomenon was well correlated with significant suppression of the overproduction of inflammation cytokines (IL-1, IL-1β, IL-6, and TNF-α), oxidant stress makers (MDA and NO), eicosanoids (LTB4 and PGE2), and inflammatory enzymes (5-LOX and COX-2) versus the AIA rats without treatment. On the contrary, the release of SOD and IL-10 was profoundly increased. What’s more, TSSN could obviously ameliorate the translocation of NF-κB to the nucleus through phosphorylation of the p65 and IκBα in vivo and vitro. The current findings demonstrated that TSSN could protect the injured ankle joint from further deterioration and exert its satisfactory anti-arthritis properties through anti-inflammatory and anti-oxidant effects via inactivating NF-κB signal pathway. This research implies that DZW may be a useful therapeutic agent for the treatment of human arthritis.

show abstract

Inhibitory effects of the root extract of Litsea cubeba (lour.) pers. on adjuvant arthritis in rats

Cited by 75 publications

References 37 publications

Anti-inflammatory constituents from the root of Litsea cubeba in LPS-induced RAW 264.7 macrophages

Anti-inflammatory constituents from the root of Litsea cubeba in LPS-induced RAW 264.7 macrophages

Pristimerin, a naturally occurring triterpenoid, protects against autoimmune arthritis by modulating the cellular and soluble immune mediators of inflammation and tissue damage

Therapeutic effects of total steroid saponin extracts from the rhizome of Dioscorea zingiberensis C.H.Wright in Freund's complete adjuvant induced arthritis in rats

Contact Info

Product

Resources

About