Inhibitory Effects of Tumor Necrosis Factor‐Alpha on Migration of Human Periodontal Ligament Cells

Takemura, Akihiro; Nakagawa, Ichirô; Kawai, Shinji; Inaba, Hiroaki; Kato, Takahiro; Hamada, Shigeyuki; Amano, Atsuo

doi:10.1902/jop.2006.050192

Cited by 14 publications

(12 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In PDL cells, there have only been a few studies to examine the mechanisms of integrin‐induced migration. In contrast to our data, increased expression of integrin α5 was shown to be involved in the migration inhibition under inflammatory conditions mediated by tumour necrosis factor‐α . Glial cell‐derived neurotrophic factor enhances the PDL cell migration via integrin αvβ3 .…”

Section: Discussioncontrasting

confidence: 99%

“…In contrast to our data, increased expression of integrin α5 was shown to be involved in the migration inhibition under inflammatory conditions mediated by tumour necrosis factor-α. 29 Glial cell-derived neurotrophic factor enhances the PDL cell migration via integrin αvβ3. 30 However, in PCR array analysis, the mRNA levels of integrin αv were similar between PDL cells stimulated with PDGF-BB and controls.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Induction of migration of periodontal ligament cells by selective regulation of integrin subunits

Kawamura

Yamamoto

Yamashiro

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

The recruitment of tissue‐resident stem cells is important for wound regeneration. Periodontal ligament cells (PDL cells) are heterogeneous cell populations with stemness features that migrate into wound sites to regenerate periodontal fibres and neighbouring hard tissues. Cell migration is regulated by the local microenvironment, coordinated by growth factors and the extracellular matrix (ECM). Integrin‐mediated cell adhesion to the ECM provides essential signals for migration. We hypothesized that PDL cell migration could be enhanced by selective expression of integrins. The migration of primary cultured PDL cells was induced by platelet‐derived growth factor‐BB (PDGF‐BB). The effects of blocking specific integrins on migration and ECM adhesion were investigated based on the integrin expression profiles observed during migration. Up‐regulation of integrins α3, α5, and fibronectin was identified at distinct localizations in migrating PDL cells. Treatment with anti‐integrin α5 antibodies inhibited PDL cell migration. Treatment with anti‐integrin α3, α3‐blocking peptide, and α3 siRNA significantly enhanced cell migration, comparable to treatment with PDGF‐BB. Furthermore, integrin α3 inhibition preferentially enhanced adhesion to fibronectin via integrin α5. These findings indicate that PDL cell migration is reciprocally regulated by integrin α3‐mediated inhibition and α5‐mediated promotion. Thus, targeting integrin expression is a possible therapeutic strategy for periodontal regeneration.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Induction of migration of periodontal ligament cells by selective regulation of integrin subunits

Kawamura

Yamamoto

Yamashiro

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…5 In present study, we found that the depletion of POSTN reduced the percentage of CD146+ cells and increased the percentage of CD34+ cells in PDLSCs, which suggested revealed that TNFα reduces migration and POSTN expression in PDL cells. 38,39 A former study reported that exogenous POSTN restores TNFα-suppressed migration of PDL cells. 36 These results indicated that suppression of PDL cell migration by TNFα may be caused by a decrease of POSTN.…”

Section: Discussionmentioning

confidence: 99%

Periostin promotes migration and osteogenic differentiation of human periodontal ligament mesenchymal stem cells via the Jun amino‐terminal kinases (JNK) pathway under inflammatory conditions

et al. 2017

View full text Add to dashboard Cite

show abstract

“…MMP expressions can be induced by various growth factors and cytokines, including epidermal growth factor [41]. The expression of integrins can also be induced by tumor necrosis factor alpha [42]. These inductions require the activation of the Rho pathway.…”

Section: Discussionmentioning

confidence: 99%

Reduction of lung metastasis, cell invasion, and adhesion in mouse melanoma by statin-induced blockade of the Rho/Rho-associated coiled-coil-containing protein kinase pathway

Kidera

Tsubaki

Yamazoe

et al. 2010

J Exp Clin Cancer Res

View full text Add to dashboard Cite

BackgroundMelanomas are highly malignant and have high metastatic potential; hence, there is a need for new therapeutic strategies to prevent cell metastasis. In the present study, we investigated whether statins inhibit tumor cell migration, invasion, adhesion, and metastasis in the B16BL6 mouse melanoma cell line.MethodsThe cytotoxicity of statins toward the B16BL6 cells were evaluated using a cell viability assay. As an experimental model, B16BL6 cells were intravenously injected into C57BL/6 mice. Cell migration and invasion were assessed using Boyden chamber assays. Cell adhesion analysis was performed using type I collagen-, type IV collagen-, fibronectin-, and laminin-coated plates. The mRNA levels, enzyme activities and protein levels of matrix metalloproteinases (MMPs) were determined using RT-PCR, activity assay kits, and Western blot analysis, respectively; the mRNA and protein levels of vary late antigens (VLAs) were also determined. The effects of statins on signal transduction molecules were determined by western blot analyses.ResultsWe found that statins significantly inhibited lung metastasis, cell migration, invasion, and adhesion at concentrations that did not have cytotoxic effects on B16BL6 cells. Statins also inhibited the mRNA expressions and enzymatic activities of matrix metalloproteinases (MMPs). Moreover, they suppressed the mRNA and protein expressions of integrin α2, integrin α4, and integrin α5 and decreased the membrane localization of Rho, and phosphorylated LIM kinase (LIMK) and myosin light chain (MLC).ConclusionsThe results indicated that statins suppressed the Rho/Rho-associated coiled-coil-containing protein kinase (ROCK) pathways, thereby inhibiting B16BL6 cell migration, invasion, adhesion, and metastasis. Furthermore, they markedly inhibited clinically evident metastasis. Thus, these findings suggest that statins have potential clinical applications for the treatment of tumor cell metastasis.

show abstract

Inhibitory Effects of Tumor Necrosis Factor‐Alpha on Migration of Human Periodontal Ligament Cells

Abstract: Our results suggested that TNF-alpha impairs cellular migration by enhancing cellular adhesive ability following significant focal adhesion and stress fiber formation.

Cited by 14 publications

References 44 publications

Induction of migration of periodontal ligament cells by selective regulation of integrin subunits

Induction of migration of periodontal ligament cells by selective regulation of integrin subunits

Periostin promotes migration and osteogenic differentiation of human periodontal ligament mesenchymal stem cells via the Jun amino‐terminal kinases (JNK) pathway under inflammatory conditions

Reduction of lung metastasis, cell invasion, and adhesion in mouse melanoma by statin-induced blockade of the Rho/Rho-associated coiled-coil-containing protein kinase pathway

Contact Info

Product

Resources

About