Inhibitory Effects of ZK 36374, a Stable Prostacyclin Analogue, on Adhesion of Rabbit Platelets to Damaged Aorta and Serotonin Release by Adherent Platelets

Abstract

The effects of ZK 36374, a prostacyclin analogue, were studied on adhesion of rabbit platelets to damaged rabbit aorta and on activation of platelets (judged by release of serotonin and formation of thromboxane-B2) in response to the processes of adhesion to the vessel surface and aggregation in response to microfibrillar collagen in suspension. In the presence of ZK 36374 (10-100 nmol/l), platelet adhesion and thromboxane-B2 formation were progressively reduced. The extent of serotonin release from adherent platelets was similar to that found for platelets aggregated with collagen. However, higher concentrations of ZK 36374 were required to inhibit serotonin release from adherent platelets than from aggregated platelets. The results indicate that ZK 36374 acts similarly to native prostacyclin on adhesion and collagen-induced aggregation and unlike previously described analogues is equipotent. The mechanism of release of serotonin induced by adhesion of platelets is less sensitive to the action of ZK 36374 than that of release induced by aggregation in response to microfibrillar collagen in suspension.

Platelet and Plasma Vasoactive Amines in Type 1 (Insulin-Dependent) Diabetes Mellitus with and without Vascular Disease

“…19 Platelet-rich plasma was separated by centrifugation at 200 g for 9 min. The platelet-containing supernatant was removed and centrifuged at 1500g for 20 min to obtain a platelet pellet.…”

Die Verlängerung der Lagerungskonservierung von Schweinenieren auf 96 Stunden durch Einsatz von Iloprost

“…Außerdem erfolgt die hypotherme Perfusion der Niere gleichmäßiger und sorgt für einen guten Wärmeaustausch, der für die nachfolgende Lagerungskonservierung bei 4 °C notwendig ist. 17,18,20,24,25,26). Die Bildung von Thrombozytenaggregaten, vor allem im Bereich der Aa.…”

Effects of Prostacyclin and of the Stable Prostacyclin Analogue ZK 36374 on Forearm Blood Flow and Blood Platelet Behaviour in Man

“…ZK 36374 (Iloprost) is a chemically stable analogue of PGI2 that also inhibits platelet aggregation. Studies of its effects on blood platelets in vitro have shown that it is at least as potent as PGI2 (5)(6)(7). However, studies in animals (8) and in man (9) have indicated that its effects on the circulation may be less profound than those of PGI2.…”