Inhibitory Mechanisms of Water Extract of Oplopanax elatus on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Murine Macrophage Cells

Lim, Hyun; Kwon, Yong Soo; Kim, Myong Jo; Yoo, Ji Hye; Yoo, Nam Ho; Kim, Hyun Pyo

doi:10.1007/s11655-020-3188-2

Cited by 5 publications

(2 citation statements)

References 23 publications

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“…In recent years, the gallic acid has been reported to inhibit NO, PGE2, and IL-6 in LPS-induced RAW 267.4 macrophages [ 11 ]. Syringin alleviated inflammation in colitis [ 12 ] and also significantly inhibited iNOS-induced NO and COX-2-induced PGE2 production in LPS-treated RAW 264.7 cells [ 13 ]. Osthole treatment also can ameliorate the inflammatory via suppress the level of the pro-inflammatory cytokines IL and blocked the activation of nuclear factor kappa B (NF-κB) [ 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

The effect and mechanism of QufengZhitong capsule for the treatment of osteoarthritis in a rat model

Cheng

Gan

et al. 2021

Journal of Orthopaedic Translation

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Objective The purpose of this study was to evaluate the therapeutic effects and mechanism of Qufeng Zhitong (QFZT)capsule for the treatment of osteoarthritis (OA) in a rat model. Methods 8-10-week-old male Sprague–Dawley rats were randomly divided into the sham group (vehicle-treated), OA group (vehicle-treated), high-dose, middle-dose, low-dose of QFZT capsule -treated groups. OA was induced by transecting the medial collateral ligament and the medial meniscus in the right limb. The Sprague–Dawley rats were treated daily for 12 weeks with different concentrations of QFZT capsule : low (QFZT-L, 128 mg/kg), medium (QFZT-M, 256.5 mg/kg), and high (QFZT-H, 513 mg/kg) by gavage administration for a period of 4 and 12 weeks respectively. Vehicle-treated rats served as controls and administered 0.5% Carboxymethyl Cellulose Sodium (CMC-Na) by gavage on the same schedule. Weekly measurement of dynamic weight-bearing capacity, grip strength, joint swelling was were performed to monitor the progression of disease for 3 weeks. After euthanasia, the knee joints were articular cartilage changes. Pro-inflammatory gene expression in synovial joints was examined to assess the bone and cartilage changes. Gene expression of pro-inflammatory cytokines in synovial joints was measured to determine the therapeutic effect of QFZT. Results 2 weeks after the treatment, the grip strength and weight-bearing capacity were significantly increased in the QFZT-M and QFZT-H groups, compared with the OA group. The joint widths were decreased significantly in the QFZT-L and QFZT- H groups, compared with the OA group as well. The mRNA level in the articular cartilage of knee joint of IL-1β in the QFZT-L group and IL-6 in the QFZT-H group was significantly suppressed at week 4, compared with the OA group. The radiology score was significantly decreased in the QFZT-H group compared with the OA group 12 weeks after treatment. Furthermore, the rats on QFZT treatment decreased the progression of OA, which was characterised by decreased cartilage degradation. However, the bone changes were no different in OA group and QFZT groups. Conclusion In a rat model of OA, QFZT capsule shows the tendency to reduce the destruction of cartilage, joint swelling and bone erosion which provides new evidence for the therapeutic potential of QFZT capsule in the treatment of OA in clinics. The translational potential of this article The QFZT capsule can improve the symptoms of the OA in rodent animal rats by attenuating pain and retarding cartilage damage. This study indicated that the QFZT capsule has the potential clinical application of in OA therapy.

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Section: Introductionmentioning

confidence: 99%

The effect and mechanism of QufengZhitong capsule for the treatment of osteoarthritis in a rat model

Cheng

Gan

et al. 2021

Journal of Orthopaedic Translation

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“…Oplopanax elatus (Nakai) Nakai has a long history of use as an ethnomedicine by the people living in eastern Asia, which was introduced in the Jilin Chinese Herbal Medicine (Changchun College of Traditional Chinese Medicine, 1970), and the claimed indication for O. elatus includes neurasthenia, schizophrenia, rheumatism, and cardiovascular diseases (Shikov et al, 2014). Data from human subjects suggest that O. elatus showed positive effects for the management of diabetes (Klimakova and Kazman, 1962), depression (Turova, 1974), rheumatic arthritis (Tian et al, 1985), and bacterial and fungal infections (Fu et al, 1997;Vereshchagin et al, 1982), with antioxidant and anti-inflammatory potentials (Kwon et al, 2020;Tian et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Falcarindiol and dichloromethane fraction are bioactive components in Oplopanax elatus: Colorectal cancer chemoprevention via induction of apoptosis and G2/M cell cycle arrest mediated by cyclin A upregulation

et al. 2021

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Oplopanax elatus (Nakai) Nakai has a long history of use as an ethnomedicine by the people living in eastern Asia. However, its bioactive constituents and cancer chemopreventive mechanisms are largely unknown. The aim of this study was to prepare O. elatus extracts, fractions, and single compounds and to investigate the herb's antiproliferative effects on colon cancer cells and the involved mechanisms of action. Two polyyne compounds were isolated from O. elatus, falcarindiol and oplopandiol. Based on our HPLC analysis, falcarindiol and oplopandiol are major constituents in the dichloromethane (CH 2 Cl 2 ) fraction. For the HCT-116 cell line, the dichloromethane fraction showed significant effects. Furthermore, the IC50 for falcarindiol and oplopandiol was 1.7 µM and 15.5 µM, respectively. In the mechanistic study, after treatment with 5 µg/ml for 48 h, dichloromethane fraction induced cancer cell apoptosis by 36.5% (p < 0.01% vs. control of 3.9%). Under the same treatment condition, dichloromethane fraction caused cell cycle arrest at the G2/M phase by 32.6% (p < 0.01% vs. control of 23.4%), supported by upregulation of key cell cycle regulator cyclin A to 21.6% (p < 0.01% vs. control of 8.6%). Similar trends were observed by using cell line HT-29. Data from this study filled the gap between phytochemical components and the cancer chemoprevention of O. elatus. The dichloromethane fraction is a bioactive fraction, and falcarindiol is identified as an active constituent. The mechanisms involved in cancer chemoprevention by O. elatus were apoptosis induction and G2/M cell cycle arrest mediated by a key cell cycle regulator cyclin A.

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Syringin: Plant Source, Traditional Uses, Anti‐Cancer, Brain Protection, and Related Pharmacological Properties

Liu,

Xu,

Wang

et al. 2024

Chemistry & Biodiversity

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Traditional herbal medicines containing syringin in different parts of the world, have been used to enhance memory, relieve pain, cough, clear fever, treat psoas tension, tonsillitis, sore throat, acute gastroenteritis, anti‐inflammatory, analgesic, etc. In this paper, the extraction, analytical method, pharmacological action and research progress of syringin containing plants were reviewed. Various extraction methods and detection methods of syringin were summarized, especially the ultrasonic‐assisted extraction and high‐performance liquid chromatography were recommended for the extraction and determination of syringin. We spotlighted the anti‐cancer, brain‐protective, and anti‐inflammatory pharmacological effects of syringin. An in‐depth analysis of four plants that contain syringin ‐‐ Eleutherococcus senticosus, Codonopsis pilosula, Daphne tangutica Maxim and Syringa reticulata subsp. Amurensis. In addition, the safety and efficacy of these four plants and preparations containing syringin (Shugan Jieyu Capsule, compound Coginseng tablet, hyoscyamine ointment and Qinfenghong Zhike capsule) were analyzed. Although syringin has been widely used in traditional medicine, its specific mechanism of action and clinical efficacy are still not completely understood, and further research is needed to explore and verify it. This study provides valuable theoretical basis and potential research direction for the research and development of new drugs such as anti‐cancer and brain protection.

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Inhibitory Mechanisms of Water Extract of Oplopanax elatus on Lipopolysaccharide-Induced Inflammatory Responses in RAW 264.7 Murine Macrophage Cells

Cited by 5 publications

References 23 publications

The effect and mechanism of QufengZhitong capsule for the treatment of osteoarthritis in a rat model

The effect and mechanism of QufengZhitong capsule for the treatment of osteoarthritis in a rat model

Falcarindiol and dichloromethane fraction are bioactive components in Oplopanax elatus: Colorectal cancer chemoprevention via induction of apoptosis and G2/M cell cycle arrest mediated by cyclin A upregulation

Syringin: Plant Source, Traditional Uses, Anti‐Cancer, Brain Protection, and Related Pharmacological Properties

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