Inhibitory potential of EGCG on Streptococcus mutans biofilm: A new approach to prevent Cariogenesis

Islam, Mohamed Ibrahim Hairul; Arokiyaraj, Selvaraj; Kuralarasan, M.; Kumar, Venugopal Senthil; Harikrishnan, Pandurangan; Saravanan, S.; Ashok, Ganapathy; Chellappandian, Muthiah; Bharanidharan, Rajaraman; Muralidaran, Senguttuvan; Thirugnanasambantham, Krishnaraj

doi:10.1016/j.micpath.2020.104129

Cited by 16 publications

(7 citation statements)

References 25 publications

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“…A highlight should be given to the residue that interacted with virtually all ligands, Trp 517, which is a vital amino acid in the active region of this polymerase [23] . The present study evaluated the binding potential of derivatives towards Tyr 430, Glu 515 and Trp 517 in S. mutans glucansucrase.…”

Section: Resultsmentioning

confidence: 99%

Campesterol Semi‐Synthetic Derivatives as Potential Antibacterial: in vitro and in silico Evaluation

Santiago

Silva

Pinto

et al. 2023

Chemistry & Biodiversity

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In this study, twelve campesterol derivatives (2-13) were prepared by esterification reaction at the hydroxy group in C-3 and catalytic hydrogenation at the carbon-carbon double bond in C-5(6). All obtained compounds were characterized by IR, 1 H-NMR, 13 C-NMR, and MS spectra. Campesterol (1) and its derivatives (2-13) were evaluated in vitro against Staphylococcus aureus (ATCC 6538), Streptococcus mutans (ATCC 0046), Escherichia coli (ATCC 10536), Pseudomonas aeruginosa (ATCC 15442), and Klebsiella pneumoniae (ATCC 10031) using the microdilution method. Among tested compounds, 4, 6, 9, 11, 12, and 13 displayed the best antibacterial activity. Moreover, to support the antibacterial activity experiments, the investigation of molecular interactions of more active compounds, and also compound 1 and neomycin, used as starting material and positive control, respectively, at the binding site of the target proteins was performed using molecular docking simulations. Four compounds (7, 9, 10 and 11) are herein described for the first time.

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Section: Resultsmentioning

confidence: 99%

Campesterol Semi‐Synthetic Derivatives as Potential Antibacterial: in vitro and in silico Evaluation

Santiago

Silva

Pinto

et al. 2023

Chemistry & Biodiversity

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“…Biofilm production by S. mutans is critical in the pathogenesis of dental plague associated oral diseases [38,39]. Several natural products have been evaluated for their anti-plaque properties in an effort to attenuate oral bacteria biofilm formation [28,38,39]. However, most of them have shown detrimental effects on the oral microbiome.…”

Section: Discussionmentioning

confidence: 99%

“…Earlier described methods [28] for docking calculations were performed with AutoDock (version 1.5.2 revision 2) and executed with AutoDock version 4.2. The mangiferin that acted as the reference drug for procedure validation was docked in to the active site.…”

Section: Molecular Dockingmentioning

confidence: 99%

“…The mangiferin that acted as the reference drug for procedure validation was docked in to the active site. In addition, the assignment of a perfect grid to each ligand and the subsequent obtaining of grid box values are as described previously [28]. Based on the binding energy, each resulting docked conformed ligand were ranked into cluster and visually analysed using the PyMOL software version 2.3.1.…”

Section: Molecular Dockingmentioning

confidence: 99%

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Inhibitory Potential of Mangiferin on Glucansucrase Producing Streptococcus mutans Biofilm in Dental Plaque

et al. 2020

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Glucansucrase secreted by Streptococcus mutans and composed of virulence genes alters oral microbiota, creating adherent environment for structural bacteria colony forming dental biofilm. The present investigation studied the inhibitory and binding potentials of mangiferin against S. mutans and its enzyme glucansucrase implicated in biofilm formation. Antibacterial activity against planktonic S. mutans was carried out. Using reverse transcription PCR, the expression of crucial virulence genes, gtfB, gtfC, gtfD, gbpB, and comDE were determined. The effect of mangiferin on teeth surfaces biofilm was ascertained by scanning electron microscopy (SEM). Docking analysis of S. mutans glucansucrase and mangiferin revealed the binding energy of −7.35 and ten hydrogen interactions. Antibacterial study revealed that mangiferin was not lethal to planktonic S. mutans, but a concentration-dependent inhibition of glucansucrase activity was observed. The inhibitory effect of water-insoluble glucan synthesis was apparently more marked relative to water-soluble glucan synthesis attenuation. Mangiferin significantly downregulated the expression of the virulence genes, indicating a mechanism involving glucanotranferases, specifically inhibiting colony formation by attenuating bacterial adherence. SEM images revealed that S. mutans biofilm density was scanty in mangiferin treated teeth compared to non-treated control teeth. Our data therefore suggest that mangiferin inhibited S. mutans biofilms formation by attenuating glucansucrase activities without affecting bacteria growth.

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“…EGCG not only inhibits planktonic bacteria but also reduces the biofilm formation of S. mutans by inhibiting Gtfs. In addition, EGCG can inhibit lactate dehydrogenase and F 1 F 0 -ATPase, and thus reduces the acidogenicity and aciduricity of S. mutans ( Xu et al., 2011 ; Xu et al., 2012 ; Hairul Islam et al., 2020 ). A lipid-soluble green tea polyphenols which is designed based on EGCG, namely epigallocatechin-3-gallate-stearate (EGCG-S), shows an increased stability and antibiofilm activity comparable to CHX ( Melok et al., 2018 ).…”

Section: Small Molecules That Inhibit Keystone Bacteria Associated Wi...mentioning

confidence: 99%

The Application of Small Molecules to the Control of Typical Species Associated With Oral Infectious Diseases

Shen

Lyu

Zhang

et al. 2022

Front. Cell. Infect. Microbiol.

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Oral microbial dysbiosis is the major causative factor for common oral infectious diseases including dental caries and periodontal diseases. Interventions that can lessen the microbial virulence and reconstitute microbial ecology have drawn increasing attention in the development of novel therapeutics for oral diseases. Antimicrobial small molecules are a series of natural or synthetic bioactive compounds that have shown inhibitory effect on oral microbiota associated with oral infectious diseases. Novel small molecules, which can either selectively inhibit keystone microbes that drive dysbiosis of oral microbiota or inhibit the key virulence of the microbial community without necessarily killing the microbes, are promising for the ecological management of oral diseases. Here we discussed the research progress in the development of antimicrobial small molecules and delivery systems, with a particular focus on their antimicrobial activity against typical species associated with oral infectious diseases and the underlying mechanisms.

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Inhibitory potential of EGCG on Streptococcus mutans biofilm: A new approach to prevent Cariogenesis

Cited by 16 publications

References 25 publications

Campesterol Semi‐Synthetic Derivatives as Potential Antibacterial: in vitro and in silico Evaluation

Campesterol Semi‐Synthetic Derivatives as Potential Antibacterial: in vitro and in silico Evaluation

Inhibitory Potential of Mangiferin on Glucansucrase Producing Streptococcus mutans Biofilm in Dental Plaque

The Application of Small Molecules to the Control of Typical Species Associated With Oral Infectious Diseases

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