Inhibitory Potential Of Lactobacillus Plantarum on Escherichia Coli

Abstract

An alternative therapy to diarrhoeal episodes induced by E. coli is represented by probiotic strains (e.g Lactobacillus), which are able to maintain the gut micro-flora in optimal parameters. This work highlights the inhibitory potential of probiotics (L. plantarum ATCC 8014) over the enteropathogenic bacteria like E. coli ATCC 25922. For this study we co-incubated L. plantarum with E. coli in MRS broth at different concentrations (10 4 CFU/mL -E. coli, 4 groups) for 24, 48 and 72h at 37 o C. After co-incubation, E. coli colonies developed on Levine media were counted towards the control. E. coli colonies decreased after 48 and 72h of co-incubation with L. plantarum. Also, was noticed that L. plantarum (10 8 CFU/mL) completely inhibits E. coli (10 4 CFU/mL) after 72h of co-incubation. This work proves the inhibitory potential of probiotic strains (L. plantarum ATCC 8014) against enteropathogenic bacteria (E. coli ATCC 25922).

Coronavirus Disease (COVID-19) Caused by (SARS-CoV-2) Infections: A Real Challenge for Human Gut Microbiota

“…RT-PCR analysis indicated the presence of SARS-CoV-2 in both the nasopharyngeal and oropharyngeal mucosa, and in diarrhea specimens, while the serum was negative for the virus ( Ding and Liang, 2020 ; Holshue et al., 2020 ). During clinical episodes of acute and/or severe diarrhea, the human GI tract is highly dehydrated and a high level of pathogen colonization and infestation is observed ( Mitrea et al., 2017 ; Da Cruz Gouveia et al., 2020 ). Severe diarrheal episodes cause a strong imbalance and dysbiosis within the intestinal ecosystem and significantly affect electrolyte availability, leading to a rapid weakening of general health ( Guyton and Hall, 2010 ).…”

Identification and probiotic properties of lactobacilli isolated from two different fermented beverages

“…Moreover, the probiotic effect of lactic fermentation products is widely recognized (Sõukand et al 2015). Results of clinical studies confirm the positive effect of probiotics on gastrointestinal and allergic diseases, and also their contribution to immunomodulation and the benefits of the prophylactic use of probiotics in different types of cancer (Stefanut et al 2015;Markoviak and Śliżewska 2017;Mitrea et al 2017). In many regions of the world, fermented foods and beverages based on milk, cereals, or other substrates, are known for their health-promoting properties, although many of them are poorly studied and the health claims are not backed by credible scientific evidence (Marsh et al 2014).…”

The Use of Chitosan, Alginate, and Pectin in the Biomedical and Food Sector—Biocompatibility, Bioadhesiveness, and Biodegradability

“…In recent years, remarkable and creative ways of utilizing biopolymer-based materials have delivered a continuous development for sustainable bioeconomy and biotechnology [33,34,35]. A large number of recent articles have led to the expansion of evidence suggesting that enzyme-mediated catalytic bioprocesses have many advantages over conventional synthetic pathways and, therefore, they are increasingly important for multiple biotechnological applications, including biocatalysis, food products, environmental protection, biomedicine, bioenergy, biosensor development, and agro-chemistry based on renewable biomaterials [36,37,38,39,40,41,42].…”