“…Seven different environments, namely, urban drinking water source (Chaohu Lake), 26 freshwater lake sediments, 27 wastewater of a wastewater treatment plant, 28 seawater, mouse feces, 29 feces of patients with rheumatoid arthritis (RA), 30 and feces of patients with colorectal cancer (CRC), 31 were selected to verify the application of NCRD in different environmental niches. The metagenomic datasets of seawater (GenBank: PRJEB1787 and PRJNA398459 ), wastewater (GenBank: PRJNA230567 ), the RA patient’s feces (GenBank: PRJEB6997 ), the CRC patient’s feces (GenBank: PRJNA531273 ), and mouse feces (GenBank: PRJEB40312 ) were downloaded from the sequence read archive of NCBI.…”