Inibição da atividade da citrato sintase cerebral em um modelo animal de sepse

Abstract

Objective: An extensive body of evidence from experimental studies indicates that sepsis is associated with increased reactive oxygen species production, depletion of antioxidants, and accumulation of markers of oxidative stress. Moreover, mitochondrial dysfunction has been implicated in the pathogenesis of multiple organ dysfunction syndrome (MODS). Citrate synthase is an enzyme localized in the mitochondrial matrix and an important component of the Krebs cycle; consequently, citrate synthase has been used as a quantitative enzyme marker for the presence of intact mitochondria. Thus, we investigated citrate synthase activity in the brains of rats submitted to a cecal ligation puncture model of sepsis.Methods: At several times points (3, 6, 12, 24 and 48 hours) after the cecal ligation puncture operation, six rats were killed by decapitation. Their brains were removed, and the hippocampus, striatum, cerebellum, cerebral cortex and prefrontal cortex were dissected and used

Supplementation with Vitamin D3 Protects against Mitochondrial Dysfunction and Loss of BDNF-Mediated Akt Activity in the Hippocampus during Long-Term Dexamethasone Treatment in Rats

“…To determine the function of mitochondria in the Krebs cycle, we examined the activity of citrate synthase (CS). CS is an enzyme localized in the mitochondrial matrix and is used as a quantitative enzymatic marker for the presence of intact mitochondria [ 65 , 66 ]. Our data indicate that long-term DEXA administration decreased CS activity in the hippocampus, while vitamin D 3 supplementation contributed to maintaining proper enzyme activity.…”