Initial applications of phospholipid-coated mercury electrodes to the determination of polynuclear aromatic hydrocarbons and other organic micropollutants in aqueous systems

Nelson, Andrew; Auffret, N.; Readman, JW

doi:10.1016/s0003-2670(00)80781-3

Cited by 38 publications

(18 citation statements)

References 6 publications

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“…Ion and charge transfer across the film, as well as the interactions in the film, has been detected by using phase-sensitive alternating current (ac) voltammetry. The lipid-coated electrode represents a very sophisticated system for studying the structure and functioning of biological membranes (19)(20)(21) and for investigating the aquatic chemistry of organic and inorganic micropollutants (22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

Interaction of Polysaccharides with Lipid Monolayers

Kozarac

Ćosović

Möbius

et al. 2000

Journal of Colloid and Interface Science

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The interactions of model neutral lipids such as lecithin and dipalmitoyl phosphatidylcholine (DPPC), and of the positively charged lipid dioctadecyl dimethyl ammonium bromide (DOMA) with neutral (dextrans) and acidic polysaccharides (carrageenans and xanthan), representing the hydrophilic solutes occurring in natural aquatic systems, have been monitored electrochemically and by monolayer techniques. Surface pressure-area (π -A) and surface potential-area ( V-A) isotherms have been measured. The values for compressibility of mixed films and the dipol moments (effective dipole moment µ T and polar head group dipole moment µ α ) have been calculated from π-A and V-A isotherms, respectively. In electrochemical experiments lipid-coated mercury electrodes have been used. The influence of polysaccharides on the monolayers structure has been studied by capacitance measurements and by using an electrochemical probe (redox processes of cadmium) with phase-sensitive ac voltammetry. The results presented here are part of more general studies of the interaction of organic solutes naturally occurring in the aquatic systems with hydrophobic lipids at different phase boundaries. These investigations are of great importance for the interfacial properties of mixed monolayers and for understanding the influence of hydrophilic and hydrophobic substances on the physicochemical properties of natural surface films. C 2000 Academic PressKey Words: polysaccharides; lipid monolayers; air/water interface; π-A and V-A isotherms; capacity measurements; ac voltammetry.

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Section: Introductionmentioning

confidence: 99%

Interaction of Polysaccharides with Lipid Monolayers

Kozarac

Ćosović

Möbius

et al. 2000

Journal of Colloid and Interface Science

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“…The response increases with increase in concentration of compound in the working solution which is injected into the flow. The response to pyrene and DDT is identical to the effect of pyrene [7,35] and DDT [7,35,36] interaction respectively on the voltammetric curve of a DOPC coated HMDE in a 75 cm 3 electrochemical cell. The effect of pyrene interaction induces the negative potential shift, potential separation and suppression of two voltammetric peaks whereas the effect of DDT interaction is to elicit a suppression of the voltammetric peaks.…”

Section: Sensor Exemplificationmentioning

confidence: 69%

High throughput systems for screening biomembrane interactions on fabricated mercury film electrodes

et al. 2011

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A sensing system for the rapid on-line screening of lipids and biomembrane active compounds in water samples has been successfully developed. The sensor consists of a flow cell and incorporated wafer-based device with on-chip mercury on platinum (Pt/Hg) working electrode and platinum (Pt) auxiliary and pseudo-reference electrodes. To optimise system performance, the following experiments were carried out: (i) Deposition and removal of phospholipid layers on and from Pt/Hg electrodes respectively, (ii) Effect of electrode size on signal, (iii) Monitoring of different phospholipids deposited in flow cell and, (iv) Detection of phospholipid monolayer interaction with representative compounds. The results showed that: (i) Miniaturisation and ruggedisation of the mercury (Hg)/phospholipid system has been successfully achieved, (ii) Rapid cyclic voltammetry facilitates repetitive dioleoyl phosphatidylcholine (DOPC) monolayer formation on Hg from aqueous DOPC dispersion and, (iii) The device responds selectively to organic compounds injected into electrolyte flow.

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“…Thus, while the effect of benzene and naphthalene is negligible, it becomes appreciable with hydrocarbons with three, four or five aromatic rings. 50 Hydrophobicity alone, as measured, say, by the octanol/water partition coefficient, is not sufficient to explain this effect; thus, undecane and dodecane have a high partition coefficient but no observable effect on lipid monolayers. The slight decrease in the flat capacitance minimum which is often observed with an increase in the bulk concentration of these compounds is probably to be ascribed to a thickening of the film following their incorporation in the lipid monolayer; this should more than compensate for the expected increase in capacitance stemming from the higher dielectric constant of aromatic compounds compared to that,  2, of the lipid tails.…”

Section: Mercury Supported Lipid Monolayersmentioning

confidence: 99%