Background: China has basically succeeded in controlling the COVID-19 epidemic, which is due to the cooperation and acceptance of epidemic prevention measures by the public. However, few studies have examined the measures China has taken to combat COVID-19 in order to reflect on its success in curbing the the spread of epidemic.Methods: In this study, the public acceptance questionnaire was designed based on the epidemic prevention measures adopted in China, to investigate the difference of public acceptance of epidemic prevention measures. The survey data was collected from 2,062 samples with different demographic characteristics from March 8, 2020 to April 9, 2020. And SPSS was used to analyze the data collected in the questionnaire, such as one-way variance, so as to draw conclusions.Results: The results show that age and educational level have a significant influence on public acceptance. In contrast gender and occupation field has no significant impact on it. The acceptance of the emergency prevention and control measures taken by the government during the epidemic period is generally high. With the development of the epidemic, the acceptability is increasing. And the public acceptance of traffic measures was highest. Conclusions: Rapid deployment of epidemic prevention measures and appropriate methods in transportation, economy and education are the key to China's effective containment of the epidemic. Measures such as shutting down cities and encouraging the wearing of masks deserve to be copied by other countries. This study summed up China's scientific experience in the fight against COVID-19 and differences in public acceptance. It can provide a positive reference for the development of epidemic prevention measures in other countries.