Initial attachment of human oral keratinocytes cultured on zirconia or titanium

Abstract

Initial attachment of human oral keratinocytes (HOKs) cultured on mirror-surfaced commercially pure titanium (Ti) or yttriastabilized tetragonal zirconia polycrystals (TZP) was investigated. Numbers of viable attached HOKs, their mRNAs and proteins expression of laminin γ2 and integrin β4 were evaluated using the WST-1 assay, quantitative real-time PCR and enzyme-linked immunosorbent assay, respectively. Localization of laminin γ2 and integrin β4 was observed using immunofluorescent staining. Cell spreading was evaluated by measuring the perimeter of actin on fluorescent stained images, and cell morphology was examined using scanning electron microscopy. At 1 h TZP elicited less of initial attachment than Ti in terms of mRNAs expression and proteins expression of laminin γ2 and integrin β4 (p<0.05). However, at 48 h TZP was showed similar initial attachment in comparison to Ti. Therefore, it was suggested that TZP has a potential to form epithelial attachment like Ti.

Adhesion Properties of Human Oral Epithelial‐Derived Cells to Zirconia

“…No significant difference was observed for integrin β 4 mRNA expression among the specimens and polystyrene at 3 or 24 hours (Figure ). When human oral keratinocytes were investigated, cells cultured on Ti for 1 hour showed a significantly higher laminin γ 2 mRNA expression compared with cells cultured on zirconia . The difference in the cell type used might explain the different results observed.…”

A review on the wettability of dental implant surfaces II: Biological and clinical aspects

“…Besides hydrophilicity, keratinocytes and fibroblasts have been found to respond to surface characteristics such as surface chemistry [96, 97] and surface topography [78, 98]. In a study that controlled surface roughness and surface wettability to isolate the effects of surface chemistry on mouse fibroblasts, Ti surfaces that were coated with TiN by physical vapor deposition were compared to surfaces that were either thermally oxidized, laser irradiated or unmodified controls [97].…”

Influence of plasma and ultraviolet treatment of zirconia on initial attachment of human oral keratinocytes: Expressions of laminin γ2 and integrin β4

“…Atsuta et al showed that peri-implant epithelium (PIE) attaches to a Ti surface by hemidesmosomes with elucidating the localization of laminin γ 2 at the implant-epithelium interface 7,8) . On TZP with a mirror-polished surface, it is reported that the initial attachment of human oral keratinocytes is equivalent to or slightly lower than that on Ti 9) . Accordingly, surface modification of zirconia is necessary to ensure biological sealing around TZP implants.…”