Purpose To assess the anterior chamber (AC) characteristics and its correlation to laser flare photometry immediately after femtosecond laser-assisted capsulotomy and photodisruption.Patients and methods The study included 97 cataract eyes (n = 97, mean age 68.6 years) undergoing femtosecond laser-assisted cataract surgery (FLACS). Three cohorts were analysed relating to the flare photometry directly post femtosecond laser treatment (flare o100 n = 28, 69.6 ± 7 years; flare 100-249 n = 47, 67.7 ± 8 years; flare 4249 photon counts per ms cohort n = 22, 68.5 ± 10 years). Flare photometry (KOWA FM-700), corneal topography (Oculus Pentacam, Germany: AC depth, volume, angle, pachymetry), axial length, pupil diameter, and endothelial cells were assessed before FLACS, immediately after femtosecond laser treatment and 1 day postoperative (LenSx Alcon, USA). Statistical data were analysed by SPSS v19.0, Inc. Results The AC depth, AC volume, AC angle, central and thinnest corneal thickness showed a significant difference between flare o100 vs flare 100-249 10 min post femtosecond laser procedure (P = 0.002, P = 0.023, P = 0.007, P = 0.003, P = 0.011, respectively). The AC depth, AC volume, and AC angle were significantly larger (P = 0.001, P = 0.007, P = 0.003, respectively) in the flare o100 vs flare 4249 cohort 10 min post femtosecond laser treatment. Conclusions A flat AC, low AC volume, and a narrow AC angle were parameters associated with higher intraocular inflammation. These criteria could be used for patient selection in FLACS to reduce postoperative intraocular inflammation.