In this work, by using the Al-Oboudi differential operator and the rule of subordination, we introduced the new subclasses D
n,ρ
Σ,
δ
(Φ) and F
n,α
Σ,
δ
(Φ) of the bi-univalent functions. Likewise, we use the Fibonacci numbers to derive the initial coefficients bounds for |a
2| and |a
3| of the bi-univalent function subclasses.