Initial report on distribution of β3‐adrenoceptor in the human female urethra

Abstract

Introduction: Past research has demonstrated that the urethral tonus is mainly under sympathetic control. Since 5 years, a beta 3-adrenoceptor (ADRB3) agonist is available in the treatment of overactive bladder syndrome. The presence of ADRB3 within the human urethra has not been demonstrated to date. Presence of ADRB3 in the urethra could influence urethral tonus. The aim of this study is to investigate the presence of ADRB3 in the human female urethra. Material and Methods: We performed anatomical studies in five female specimens. Three specimens were obtained from the body donation program, two from female patients with muscle-invasive bladder cancer, where radical resection of bladder and urethra was performed. The urethra up till the bladder neck was separated from the rest of the bladder and freshly obtained for this study. For demonstrating ADRB3 expression, we used rabbit polyclonal antihuman ADRB3 LS-A4198. Results: Expression of ADBR3 was demonstrated in the epithelial layer of all urethral parts, except at the level of the meatus. The level of ADRB3 expression was highest in the mid urethra. There was no direct contact between ADRB3 and nerve tissue. ADRB3 expression was also demonstrated in the stratified muscle layer at the level of the external urethral sphincter. Conclusions: This is the first study to demonstrate the expression of ADRB3 in the human female urethra. There is an absence of a direct connection between ADRB3 and nerve tissue.

Effects of a new selective β3‐adrenoceptor agonist, vibegron, on bladder and urethral function in a rat model of Parkinson's disease

“…Kummeling et al previously reported that β 3 -AR is expressed in the human female urethra. 14 However, the potential roles of β 3 -AR in the urethral function have not been fully explored. Now that selective β 3 agonists are currently available for treatment in patients with OAB, the question regarding whether and how UP is affected by these agents arises.…”

“…Kummeling et al previously reported that β 3 ‐AR is expressed in the human female urethra 14 . However, the potential roles of β 3 ‐AR in the urethral function have not been fully explored.…”

“…Throughout the entire length of the urethra, the administration of noradrenaline or β 2 ‐AR agonist results in a contractile response. Kummeling et al recently reported that β 3 ‐AR is expressed in the human female urethra 14 . However, little research has been conducted on the effects of β 3 ‐AR on the urethra.…”

“…Kummeling et al recently reported that β 3 -AR is expressed in the human female urethra. 14 However, little research has been conducted on the effects of β 3 -AR on the urethra. Given this background, we aimed to evaluate the effects of vibegron on bladder and urethral function in a PD rat model.…”