Initial results from the Sherbrooke avalanche photodiode positron tomograph

Lecomte, Roger; Cadorette, J.; Rodrigue, S.; Lapointe, D.; Rouleau, D.; Bentourkia, M’hamed; Yao, Rutao; Msaki, P.

doi:10.1109/23.507252

Cited by 247 publications

(106 citation statements)

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“…The validation of the protocol was done in healthy rats on the Sherbrooke Small Animal PET Scanner [14] capable of achieving an in-plane resolution of 2.1 mm. The application of the protocol to the assessment of doxorubicin cardiotoxicity was performed on a LabPET4 scanner [15,16] with a transaxial and axial field of view (FOV) of 11 cm and 3.75 cm, respectively, and a nearly isotropic resolution of 1.35 mm.…”

Section: Pet Imagingmentioning

confidence: 99%

[11C]Acetate rest–stress protocol to assess myocardial perfusion and oxygen consumption reserve in a model of congestive heart failure in rats

Croteau¹,

Gascon²,

Bentourkia³

et al. 2012

Nuclear Medicine and Biology

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This study describes an [ 11 C]acetate rest/stress method to obtain an indirect estimate of myocardial blood flow (MBF) and myocardial oxygen consumption (MVO 2 ) in rats. Doxorubicin cardiotoxicity was used to test the usefulness of this approach for the assessment of congestive heart failure. Methods-[11 C]acetate rest-stress have been used in clinical research to assess the capacity of the coronary arteries to respond to stress. In this article we used this approach to assess the cardiotoxicity of doxorubicin in a rat model. The method was first validated in a group of healthy rats and then used to follow the effect of doxorubicin chemotherapy on cardiac function. The effect of doxorubicin on myocardial perfusion and oxygen consumption reserve was measured at rest and under dobutamine stimulation.Results-Validation of the protocol showed a good correlation between the MBF and MVO 2 (r 2 = 0.68). The doxorubicin treated group showed a significant (p=0.04) decrease in cardiovascular perfusion reserve at 1.3 ± 0.2 compared to the control animals at 1.6 ± 0.2. Similar results were obtained for the myocardial oxygen consumption reserve (treated 1.8 ± 0.4 vs controls 2.3 ± 0.3; p=0.02).Conclusions-We describe an [ 11 C]acetate PET rest/stress protocol for the assessment of cardiotoxicity in rat and its application to the follow-up of doxorubicin chemotherapy. This is a CIHR Author ManuscriptCIHR Author Manuscript CIHR Author Manuscript rapid and reliable approach to the measurement of cardiac perfusion and oxygen consumption reserve that could be applied to the development of new strategies to reduce the cardiotoxicity of anthracycline.

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Section: Pet Imagingmentioning

confidence: 99%

[11C]Acetate rest–stress protocol to assess myocardial perfusion and oxygen consumption reserve in a model of congestive heart failure in rats

Croteau¹,

Gascon²,

Bentourkia³

et al. 2012

Nuclear Medicine and Biology

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“…PET imaging was performed with the Sherbrooke avalanche photodiode PET scanner (University of Sherbrooke, Quebec, Canada) (Lecomte et al, 1996), achieving 2.1 mm resolution in-plane (14 ml volumetric resolution). The scanner is made of four detector rings with a total of 512 pixel detectors defining an axial field-of-view of 2.5 cm with a diameter up to 10 cm.…”

Section: Pet Imagingmentioning

confidence: 99%

Vascular-targeted photodynamic therapy with BF2-chelated Tetraaryl-Azadipyrromethene agents: a multi-modality molecular imaging approach to therapeutic assessment

et al. 2009

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BACKGROUND: Photodynamic therapy (PDT) is a treatment modality for a range of diseases including cancer. The BF 2 -chelated tetraaryl-azadipyrromethenes (ADPMs) are an emerging class of non-porphyrin PDT agent, which have previously shown excellent photochemical and photophysical properties for therapeutic application. Herein, in vivo efficacy and mechanism of action studies have been completed for the lead agent, ADMP06. METHODS: A multi-modality imaging approach was employed to assess efficacy of treatment, as well as probe the mechanism of action of ADPM06-mediated PDT. RESULTS: Tumour ablation in 71% of animals bearing mammary tumours was achieved after delivery of 2 mg kg À1 of ADPM06 followed immediately by light irradiation with 150 J cm À2 . The inherent fluorescence of ADPM06 was utilised to monitor organ biodistribution patterns, with fluorescence reaching baseline levels in all organs within 24 h. Mechanism of action studies were carried out using dynamic positron emission tomography and magnetic resonance imaging techniques, which, when taken together, indicated a decrease in tumour vascular perfusion and concomitant reduction in tumour metabolism over time after treatment. CONCLUSION: The encouraging treatment responses in vivo and vascular-targeting mechanism of action continue to indicate therapeutic benefit for this new class of photosensitiser.

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“…As an alternative, positron-emission tomography (PET), an imaging technology designed to use compounds labeled with positron-emitting radioisotopes to image and measure biochemical processes in vivo, has been adapted to use in small animals. The implementation of several high spatial-resolution PET scanners within the last several years (2)(3)(4)(5)(6)(7)(8)(9) has enabled the adaptation of the 2-deoxy-D-[ 14 C]glucose method to in vivo imaging of small animals by using 2-[ 18 F]fluoro-2-deoxy-D-glucose (FDG) as a tracer. However, such PET scanners require high counting statistics for image reconstruction that strongly reduce the possibility of performing high temporal-resolution measurements.…”

mentioning

confidence: 99%

In vivo quantification of localized neuronal activation and inhibition in the rat brain using a dedicated high temporal-resolution β ⁺ -sensitive microprobe

Pain¹,

Besret²,

Vaufrey³

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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Understanding brain disorders, the neural processes implicated in cognitive functions and their alterations in neurodegenerative pathologies, or testing new therapies for these diseases would benefit greatly from combined use of an increasing number of rodent models and neuroimaging methods specifically adapted to the rodent brain. Besides magnetic resonance (MR) imaging and functional MR, positron-emission tomography (PET) remains a unique methodology to study in vivo brain processes. However, current high spatialresolution tomographs suffer from several technical limitations such as high cost, low sensitivity, and the need of restraining the animal during image acquisition. We have developed a ␤ ؉ -sensitive high temporal-resolution system that overcomes these problems and allows the in vivo quantification of cerebral biochemical processes in rodents. This ␤-MICROPROBE is an in situ technique involving the insertion of a fine probe into brain tissue in a way very similar to that used for microdialysis and cell electrode recordings. In this respect, it provides information on molecular interactions and pathways, which is complementary to that produced by these technologies as well as other modalities such as MR or fluorescence imaging. This study describes two experiments that provide a proof of concept to substantiate the potential of this technique and demonstrate the feasibility of quantifying brain activation or metabolic depression in individual living rats with 2-[ 18 F]fluoro-2-deoxy-D-glucose and standard compartmental modeling techniques. Furthermore, it was possible to identify correctly the origin of variations in glucose consumption at the hexokinase level, which demonstrate the strength of the method and its adequacy for in vivo quantitative metabolic studies in small animals.A lterations in local cerebral metabolic rate of glucose (lCMRglc) have been reported in several human brain conditions such as psychiatric disorders or neurodegenerative diseases. Understanding the cellular mechanisms involved in these diseases and the development of new therapeutic strategies will advance more rapidly through the use of animal models. The quantitation of metabolic rates in the rodent brain was achieved initially by using the 2-deoxy-D-[ 14 C]glucose autoradiographic method (1). Although efficient, this technique requires the sacrifice of several animals to obtain each time point and thus can provide only ex vivo, averaged kinetic constants calculated from values obtained from various animals. As an alternative, positron-emission tomography (PET), an imaging technology designed to use compounds labeled with positron-emitting radioisotopes to image and measure biochemical processes in vivo, has been adapted to use in small animals. The implementation of several high spatial-resolution PET scanners within the last several years (2-9) has enabled the adaptation of the 2-deoxy-D-[ 14 C]glucose method to in vivo imaging of small animals by using 2-[ 18 F]fluoro-2-deoxy-D-glucose (FDG) as a tracer. However, such PE...

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Initial results from the Sherbrooke avalanche photodiode positron tomograph

Cited by 247 publications

References 10 publications

[11C]Acetate rest–stress protocol to assess myocardial perfusion and oxygen consumption reserve in a model of congestive heart failure in rats

[11C]Acetate rest–stress protocol to assess myocardial perfusion and oxygen consumption reserve in a model of congestive heart failure in rats

Vascular-targeted photodynamic therapy with BF2-chelated Tetraaryl-Azadipyrromethene agents: a multi-modality molecular imaging approach to therapeutic assessment

In vivo quantification of localized neuronal activation and inhibition in the rat brain using a dedicated high temporal-resolution β ⁺ -sensitive microprobe

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Initial results from the Sherbrooke avalanche photodiode positron tomograph

Cited by 247 publications

References 10 publications

[11C]Acetate rest–stress protocol to assess myocardial perfusion and oxygen consumption reserve in a model of congestive heart failure in rats

[11C]Acetate rest–stress protocol to assess myocardial perfusion and oxygen consumption reserve in a model of congestive heart failure in rats

Vascular-targeted photodynamic therapy with BF2-chelated Tetraaryl-Azadipyrromethene agents: a multi-modality molecular imaging approach to therapeutic assessment

In vivo quantification of localized neuronal activation and inhibition in the rat brain using a dedicated high temporal-resolution β + -sensitive microprobe

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In vivo quantification of localized neuronal activation and inhibition in the rat brain using a dedicated high temporal-resolution β ⁺ -sensitive microprobe