Initial Transcriptomic Response and Adaption of Listeria monocytogenes to Desiccation on Food Grade Stainless Steel

Kragh, Martin Laage; Hansen, Lisbeth Truelstrup

doi:10.3389/fmicb.2019.03132

Cited by 17 publications

(18 citation statements)

References 120 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, deletion of cspA only in the more desiccation-resistant food and outbreak-related L. monocytogenes strains (568 and 08-5578) had no impact on desiccation tolerance, although compared to the parent strains, the mutants were also nonmotile [ 19 ]. Expression of cspA was upregulated in wild-type EGDe, 568 and 08-5578 strains in response to desiccation stress confirming a role for cspA during desiccation survival [ 19 , 92 ]. Therefore, the fact that cspA deletion had no impact on 568 and 08-5578 strains desiccation stress sensitivity is indicative that functional redundancy might exist in these two strains regarding desiccation, which might not exist in EGDe.…”

Section: Role Of Csps In L Monocytogenes Stress Tolerancementioning

confidence: 95%

“…These tolerance phenotypes could also be related to loss or reduction of motility in these mutants. Other studies have suggested that decreased or absence of motility might favor enhanced desiccation tolerance as enormous cellular resources are expanded to build and run the motility apparatus [ 91 , 92 ]. However, deletion of cspA only in the more desiccation-resistant food and outbreak-related L. monocytogenes strains (568 and 08-5578) had no impact on desiccation tolerance, although compared to the parent strains, the mutants were also nonmotile [ 19 ].…”

Section: Role Of Csps In L Monocytogenes Stress Tolerancementioning

confidence: 99%

See 1 more Smart Citation

Listeria monocytogenes Cold Shock Proteins: Small Proteins with A Huge Impact

2021

View full text Add to dashboard Cite

Listeria monocytogenes has evolved an extensive array of mechanisms for coping with stress and adapting to changing environmental conditions, ensuring its virulence phenotype expression. For this reason, L. monocytogenes has been identified as a significant food safety and public health concern. Among these adaptation systems are cold shock proteins (Csps), which facilitate rapid response to stress exposure. L. monocytogenes has three highly conserved csp genes, namely, cspA, cspB, and cspD. Using a series of csp deletion mutants, it has been shown that L. monocytogenes Csps are important for biofilm formation, motility, cold, osmotic, desiccation, and oxidative stress tolerance. Moreover, they are involved in overall virulence by impacting the expression of virulence-associated phenotypes, such as hemolysis and cell invasion. It is postulated that during stress exposure, Csps function to counteract harmful effects of stress, thereby preserving cell functions, such as DNA replication, transcription and translation, ensuring survival and growth of the cell. Interestingly, it seems that Csps might suppress tolerance to some stresses as their removal resulted in increased tolerance to stresses, such as desiccation for some strains. Differences in csp roles among strains from different genetic backgrounds are apparent for desiccation tolerance and biofilm production. Additionally, hierarchical trends for the different Csps and functional redundancies were observed on their influences on stress tolerance and virulence. Overall current data suggest that Csps have a wider role in bacteria physiology than previously assumed.

show abstract

Section: Role Of Csps In L Monocytogenes Stress Tolerancementioning

confidence: 95%

Section: Role Of Csps In L Monocytogenes Stress Tolerancementioning

confidence: 99%

Listeria monocytogenes Cold Shock Proteins: Small Proteins with A Huge Impact

2021

View full text Add to dashboard Cite

show abstract

“…It is intriguing to find out whether drying for 16–24 h used in our study could have enhanced the resistance of Lm to QAC. Transcriptomic analysis of Lm desiccated on stainless steel showed that genes involved in response to other stresses were also upregulated [ 38 ]. Desiccation-treated Salmonella showed enhanced tolerance to multiple disinfectants, such as QAC, ethanol, sodium hypochlorite, hydrogen peroxide, and bile salts [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

Comparison of three neutralizing broths for environmental sampling of low levels of Listeria monocytogenes desiccated on stainless steel surfaces and exposed to quaternary ammonium compounds

Xian

Kwon

et al. 2020

BMC Microbiol

View full text Add to dashboard Cite

Background An effective environmental sampling method involves the use of a transport/neutralizing broth with the ability to neutralize sanitizer residues that are collected during sampling and to maintain viability of stressed Listeria monocytogenes (Lm) cells. Results We applied Lm onto stainless steel surfaces and then subjected Lm to desiccation stress for 16–18 h at room temperature (RT, 21–24 °C). This was followed by the subsequent application of Whisper™ V, a quaternary ammonium compound (QAC)-based sanitizer, diluted to 400 ppm and 8000 ppm of active quat, for 6 h. We then sampled Lm with sponges pre-moistened in three transport broths, Dey/Engley (D/E) broth, Letheen broth and HiCap™ broth, to generate environmental samples that contained sanitizer residues and low levels of stressed Lm, which were subsequently analyzed by an enrichment-based method. This scheme conformed with validation guidelines of AOAC International by using 20 environmental test portions per broth that contained low levels of Lm such that not all test portions were positive (i.e., fractional positive). We showed that D/E broth, Letheen broth and HiCap™ broth performed similarly when no quat or 400 ppm of quat was applied to the Lm contaminating stainless steel surfaces. However, when 8000 ppm of quat was applied, Letheen broth did not effectively neutralize the QAC in the samples. These comparisons were performed on samples stored under three conditions after collection to replicate scenarios of sample transport, RT for 2 h, 4 °C for 24 h and 4 °C for 72 h. Comparisons under the three different scenarios generally reached the same conclusions. In addition, we further demonstrated that storing Letheen and HiCap™ broths at RT for two months before sampling did not reduce their capacity to neutralize sanitizers. Conclusions We developed a scheme to evaluate the ability of transport broths to neutralize QAC sanitizers. The three transport broths performed similarly with a commonly used concentration of quat, but Letheen broth could not effectively neutralize a very high concentration of QAC. The performance of transport broths was not significantly affected under the assessed pre-sampling and post-sampling storage conditions.

show abstract

“…When L. monocytogenes was desiccated for short and extended timepoints on steel plates, it was revealed that 16 CDGC genes altered in expression (11 upregulated and five downregulated). Interestingly, both eutV/eutW were downregulated in all five measured timepoints (Kragh and Truelstrup Hansen, 2019). Maintaining food at cold temperatures is another prominent method used for controlling the growth of pathogens and other spoilage organisms.…”

Section: Implications For Survival In Food and Food Production Enviromentioning

confidence: 91%

“…These strains often harbor genes that confer increased tolerance to stresses associated with these habitats, i.e., protection against methods used to control microbial growth in food production operations. The literature strongly suggests that the CDGC may provide survival advantages to L. monocytogenes during exposure to disinfectants, growth inhibitors, desiccation, and during cold temperature (Chan et al, 2008;Fox et al, 2011;Mattila et al, 2012;Tessema et al, 2012;Hingston et al, 2017;Suo et al, 2018;Kragh and Truelstrup Hansen, 2019;Cortes et al, 2020).…”

Section: Implications For Survival In Food and Food Production Enviromentioning

confidence: 99%

The Cobalamin-Dependent Gene Cluster of Listeria monocytogenes: Implications for Virulence, Stress Response, and Food Safety

2020

View full text Add to dashboard Cite

Several genes of the eut, pdu, and cob/cbi operons are responsible for the metabolism of ethanolamine (EA) and 1,2-propanediol (PD) and are essential during the pathogenic lifecycles of various enteric pathogens. Studies concerning EA and PD metabolism have primarily focused on bacterial genera from the family Enterobacteriaceae, especially the genus Salmonella. Listeria monocytogenes is a member of the Firmicutes phylum and is the causative agent of the rare but highly fatal foodborne disease listeriosis. The eut, pdu, and cob/cbi operons are organized as a single large locus collectively referred to as the cobalamin-dependent gene cluster (CDGC). The CDGC is well conserved in L. monocytogenes; however, functional characterization of the genes in this cluster and how they may contribute to Listeria virulence and stress tolerance in food production environments is highly limited. Previous work suggests that the degradation pathway of PD is essential for L. monocytogenes establishment in the gastrointestinal tract. In contrast, EA metabolism may be more important during intracellular replication. Other studies indicate that the CDGC is utilized when L. monocytogenes is exposed to food and food production relevant stress conditions. Perhaps most noteworthy, L. monocytogenes exhibits attenuated growth at cold temperatures when a key EA utilization pathway gene was deleted. This review aims to summarize the current knowledge of these pathways in L. monocytogenes and their significance in virulence and stress tolerance, especially considering recent developments.

show abstract

Initial Transcriptomic Response and Adaption of Listeria monocytogenes to Desiccation on Food Grade Stainless Steel

Cited by 17 publications

References 120 publications

Listeria monocytogenes Cold Shock Proteins: Small Proteins with A Huge Impact

Listeria monocytogenes Cold Shock Proteins: Small Proteins with A Huge Impact

Comparison of three neutralizing broths for environmental sampling of low levels of Listeria monocytogenes desiccated on stainless steel surfaces and exposed to quaternary ammonium compounds

The Cobalamin-Dependent Gene Cluster of Listeria monocytogenes: Implications for Virulence, Stress Response, and Food Safety

Contact Info

Product

Resources

About