Initiation and resolution of interhomolog connections: crossover and non-crossover sites along mouse synaptonemal complexes

Møens, Peter B.; Marcon, Edyta; Shore, Joel S.; Kochakpour, Nazafarin; Spyropoulos, Barbara

doi:10.1242/jcs.03394

Cited by 92 publications

(126 citation statements)

References 57 publications

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“…In mice, these are the MutL homologues MLH1 and MLH3. They are essential for formation of class I cross-over which display positive interference 13 (Baker et al 1996;Moens et al 2002;Kolas and Cohen 2004;Moens et al 2007). It is possible that the CR can act as a platform for the recruitment of proteins of the homologous recombination machinery close to the DNA.…”

Section: Third Step: the Mature Scmentioning

confidence: 99%

“…DNA double strand breaks (DSBs) are initially introduced in the recipient chromosome and become repaired step-by-step by different protein complexes which use the homologous donor chromosome as a repair template and manifest themselves as molecular identifiable and distinguishable recombination nodules at or between the chromosome axes. Different models 4 explain the possible ways of repair that lead to either reciprocal exchange of chromosome fragments, called cross-over, or non-cross-over 5 (De Massy 2003;Moens et al 2007). …”

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Evolutionary history of the mammalian synaptonemal complex

et al. 2016

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Section: Third Step: the Mature Scmentioning

confidence: 99%

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confidence: 99%

Evolutionary history of the mammalian synaptonemal complex

et al. 2016

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“…In pachytene spermatocytes, Mlh3 protein is bound to the Msh4 protein. Because Msh4 is involved in resolving Holiday junctions, the function of Mlh3 may extend beyond its well established role in mismatch DNA repair to the repair of DNA double-strand breaks formed during homologous recombination (19,20). The stage-regulated increase in expression of Mlh3 is accompanied by increased expression of Parp2 [poly(ADP-ribose) polymerase family, member 2], which is involved in base excision repair (21) (Fig.…”

Section: Analysis Of Defined Stages Of the Cycle Of The Seminiferous mentioning

confidence: 99%

Stage-specific gene expression is a fundamental characteristic of rat spermatogenic cells and Sertoli cells

Johnston¹,

Wright

DiCandeloro³

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

140

141

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Mammalian spermatogenesis is a complex biological process that occurs within a highly organized tissue, the seminiferous epithelium. The coordinated maturation of spermatogonia, spermatocytes, and spermatids suggests the existence of precise programs of gene expression in these cells and in their neighboring somatic Sertoli cells. The objective of this study was to identify the genes that execute these programs. Rat seminiferous tubules at stages I, II-III, IV-V, VI, VIIa,b, VIIc,d, VIII, IX-XI, XII, and XIII-XIV of the cycle were isolated by microdissection, whereas Sertoli cells, spermatogonia plus early spermatocytes, pachytene spermatocytes, and round spermatids were purified from enzymatically dispersed testes. Microarray analysis by using Rat Genome 230 2.0 arrays identified 16,971 probe sets that recognized testicular transcripts, and 398 of these were identified as testis-specific. Expression of 1,286 probe sets were found to differ at least 4-fold between two cell types and also across the stages of the cycle. Pathway and annotated cluster analyses of those probe sets predicted that entire biological pathways and processes are regulated cyclically in specific cells. Important among these are the cell cycle, DNA repair, and embryonic neuron development. Taken together, these data indicate that stage-regulated gene expression is a widespread and fundamental characteristic of spermatogenic cells and Sertoli cells.array analysis ͉ spermatogenesis ͉ seminiferous tubules ͉ stages of the cycle of the seminiferous epithellum ͉ contraception I n mammals, spermatogenesis encompasses a series of precisely timed cellular events that take place in a highly organized tissue, the seminiferous epithelium. Within each cross-section of this tissue, spermatogonia, spermatocytes, and spermatids are in intimate physical association with somatic Sertoli cells, and, together, these cells progress synchronously through the stages of the cycle of the seminiferous epithelium (1, 2). Four cycles are required for spermatogonia, and their progeny to complete spermatogenesis. The synchrony of germ cell development has a significant effect on the structures and functions of both the germ cells and their associated somatic Sertoli cells, suggesting the existence of precise and coordinated cyclic programs of gene expression. However, because investigators have evaluated the stage-dependent changes in expression of only a few genes, whether or not cyclic gene expression is a rare or prevalent characteristic of these cells is unknown (3-7). It is also not known whether genes encoding components of important biological pathways and processes are coordinately regulated in a cyclic manner. However, such genes may represent new targets for the development of male contraceptives.Given the coordinated nature of spermatogenesis, we hypothesized that the expression of large numbers of genes differ substantially, both between specific cell types within the seminiferous epithelium and within a given cell type as it progresses through the stages of...

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“…Two factors make direct meiotic DSB identification in the mouse much more difficult compared with yeast. First, although the number of DSBs introduced per meiosis is similar in mice and yeast (Buhler et al 2007;Moens et al 2007;Mancera et al 2008), the mouse genome is 200 times larger. Second, unlike yeast, where cells can be induced to synchronously enter sporulation and initiate meiosis (Govin and Berger 2009), meiotic progression is asynchronous in mammals and at any given moment <2% of the cells in the mouse gonads contain DSBs (Bellve et al 1977;Meistrich 1977).…”

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confidence: 99%

Sensitive mapping of recombination hotspots using sequencing-based detection of ssDNA

Khil¹,

Smagulova²,

Brick³

et al. 2012

Genome Res.

121

139

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Meiotic DNA double-stranded breaks (DSBs) initiate genetic recombination in discrete areas of the genome called recombination hotspots. DSBs can be directly mapped using chromatin immunoprecipitation followed by sequencing (ChIPseq). Nevertheless, the genome-wide mapping of recombination hotspots in mammals is still a challenge due to the low frequency of recombination, high heterogeneity of the germ cell population, and the relatively low efficiency of ChIP. To overcome these limitations we have developed a novel method-single-stranded DNA (ssDNA) sequencing (SSDS)-that specifically detects protein-bound single-stranded DNA at DSB ends. SSDS comprises a computational framework for the specific detection of ssDNA-derived reads in a sequencing library and a new library preparation procedure for the enrichment of fragments originating from ssDNA. The use of our technique reduces the nonspecific double-stranded DNA (dsDNA) background >10-fold. Our method can be extended to other systems where the identification of ssDNA or DSBs is desired.

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Initiation and resolution of interhomolog connections: crossover and non-crossover sites along mouse synaptonemal complexes

Abstract: SummaryInitiation and resolution of interhomolog connections: crossover and non-crossover sites along mouse synaptonemal complexes

Cited by 92 publications

References 57 publications

Evolutionary history of the mammalian synaptonemal complex

Evolutionary history of the mammalian synaptonemal complex

Stage-specific gene expression is a fundamental characteristic of rat spermatogenic cells and Sertoli cells

Sensitive mapping of recombination hotspots using sequencing-based detection of ssDNA

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