Initiation of apoptosis, cell cycle arrest and autophagy of esophageal cancer cells by dihydroartemisinin

Du, Xiaoxue; Li, Yanjing; Wu, Chunlong; Zhou, Jianhua; Han, Yu; Sui, Hong; Wei, Xiaoli; Liu, Lei; Huang, Ping; Hong, Yuan; Zhang, Tingting; Zhang, Wenjie; Xie, Rui; Lang, Xiao-Hui; Jia, Dexin; Bai, Yuxian

doi:10.1016/j.biopha.2013.01.013

Cited by 54 publications

(43 citation statements)

References 30 publications

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“…Indeed, one recent report showed that ART induces autophagy via up-regulation of Beclin 1 expression (16). Moreover, our findings are consistent with three other reports suggesting that DHA was able to induce autophagy in cancer cells (57)(58)(59). DHA is a derivative of artemisinin, with a mechanistic action similar to that of ART (50,60).…”

Section: Discussionsupporting

confidence: 82%

Artesunate Induces Cell Death in Human Cancer Cells via Enhancing Lysosomal Function and Lysosomal Degradation of Ferritin

Yang

Tan

et al. 2014

Journal of Biological Chemistry

142

110

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Background: Artesunate is capable of inducing cell death in cancer cells. Results: Artesunate accumulates in lysosomes and promotes lysosomal function and ferritin degradation, leading to mitochondrial reactive oxygen species production and eventually cell death. Conclusion: Intracellular iron and ferritin degradation is essential for artesunate-induced lysosomal activation and cell death. Significance: This work reveals a novel mechanism underlying artesunate-induced cell death.

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Section: Discussionsupporting

confidence: 82%

Artesunate Induces Cell Death in Human Cancer Cells via Enhancing Lysosomal Function and Lysosomal Degradation of Ferritin

Yang

Tan

et al. 2014

Journal of Biological Chemistry

142

110

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“…The data demonstrated that DHA synergizes with rapamycin and promotes cell death and apoptosis via the upregulation of DAPK and Atg7, which provides an indication for its usefulness in anticancer studies and clinical application. In accordance with the results of other studies regarding DHA, the effects of DHA on autophagy were further confirmed (28)(29)(30)(31)(32). The present study provides novel evidence regarding the effects of rapamycin combined with DHA and Atg7 knockdown; however, owing to the limitations of in vitro experiments, the associated combined effects should be investigated in in vivo animal models in the future, with further cell lines investigated.…”

Section: Discussionsupporting

confidence: 90%

“…In recent years, the anticancer effects of DHA have been demonstrated in a number of studies (28,29). Zhang et al (30) demonstrated that DHA can inhibit translationally controlled tumor protein (TCTP)-dependent cell migration and invasion by inhibiting TCTP and reducing cell division control protein 42 homolog (Cdc42) activation in the TCTP-positive cell lines NOZ, GBC-SD, OCUG-1 and EH-GB-1 in vitro.…”

Section: Discussionmentioning

confidence: 99%

“…Consistent with these previous studies, the present study identified that DHA induced autophagy. Despite the presence of numerous studies (28)(29)(30)(31)(32), there is limited knowledge regarding the effect of autophagy on the anticancer action of DHA. The present study revealed the role of autophagy in the anticancer action of DHA within MDA-MB-231 cells.…”

Section: Discussionmentioning

confidence: 99%

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Rapamycin promotes the anticancer action of dihydroartemisinin in breast cancer MDA-MB-231 cells by regulating expression of Atg7 and DAPK

Liu

Zhou

et al. 2018

Oncol Lett

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Abstract. There is limited knowledge regarding the influence of autophagy on the anticancer effect of dihydroartemisinin (DHA). The present study aimed to investigate this influence within human breast cancer cells. Changes in cell viability, cell cycle distribution, apoptosis and associated genes were analyzed in MDA-MB-231 cells subjected to DHA following alteration in autophagy levels; the autophagy level was decreased following autophagy-related 7 (Atg7) knockdown or increased using rapamycin. The data indicated that rapamycin had the ability to notably enhance the anticancer effect of DHA on MDA-MB-231 cells. Autophagy induction may be key in mediating the anticancer effects of DHA, and rapamycin may regulate the death-associated protein kinase via the alteration of Atg7 expression, which would influence cell apoptosis. The present study presented a novel insight into enhancing the effectiveness of future treatment regimens for breast cancer using DHA.

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“…In a previous study, we demonstrated that DHA might be a novel agent against esophageal cancer [17]. Furthermore, DHA inactivated NF-κB and potentiated the anti-tumor effect of gemcitabine on pancreatic cancer [14].…”

Section: Introductionmentioning

confidence: 99%

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-κB in Eca109 and Ec9706 Esophageal Cancer Cells

Zhou

et al. 2014

Cell Physiol Biochem

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Background: Photodynamic therapy (PDT) is a new treatment for esophageal cancer which has been shown to be effective in the elimination of tumor. However, PDT could induce the activation of nuclear factor-kappa B (NF-κB) in many photosensitizers based PDT, which plays a negative role in PDT. In addition, our previous results have shown that dihydroartemisinin (DHA), which was the most potent one of artemisinin derivatives, has anticancer activity in esophageal cancer cells. Methods: Cell viability was determined by MTT analysis, and apoptosis was evaluated by flow cytometry. Nuclear extract was obtained for determining NF-κB DNA-binding activity, while total protein extract obtained for downstream gene expression by western blot. Results: We demonstrated DHA enhanced PDT-induced growth inhibition and apoptosis in both human esophageal cancer cell lines Eca109 and Ec9706 in vitro. The mechanism was at least partially due to DHA deactivated PDT-induced NF-κB activation, so as to decrease tremendously the expression of its target gene Bcl-2. Conclusion: Our results demonstrate that DHA augments PDT-induced growth inhibition and apoptosis in esophageal cancer cells, and that inactivation of NF-κB activity is a potential mechanism by which DHA sensitizes esophageal cancer cells to PDT-induced growth inhibition and apoptosis.

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Initiation of apoptosis, cell cycle arrest and autophagy of esophageal cancer cells by dihydroartemisinin

Cited by 54 publications

References 30 publications

Artesunate Induces Cell Death in Human Cancer Cells via Enhancing Lysosomal Function and Lysosomal Degradation of Ferritin

Artesunate Induces Cell Death in Human Cancer Cells via Enhancing Lysosomal Function and Lysosomal Degradation of Ferritin

Rapamycin promotes the anticancer action of dihydroartemisinin in breast cancer MDA-MB-231 cells by regulating expression of Atg7 and DAPK

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-κB in Eca109 and Ec9706 Esophageal Cancer Cells

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Initiation of apoptosis, cell cycle arrest and autophagy of esophageal cancer cells by dihydroartemisinin

Cited by 54 publications

References 30 publications

Artesunate Induces Cell Death in Human Cancer Cells via Enhancing Lysosomal Function and Lysosomal Degradation of Ferritin

Artesunate Induces Cell Death in Human Cancer Cells via Enhancing Lysosomal Function and Lysosomal Degradation of Ferritin

Rapamycin promotes the anticancer action of dihydroartemisinin in breast cancer MDA-MB-231 cells by regulating expression of Atg7 and DAPK

Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-&#954;B in Eca109 and Ec9706 Esophageal Cancer Cells

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Dihydroartemisinin Accentuates the Anti-Tumor Effects of Photodynamic Therapy via Inactivation of NF-κB in Eca109 and Ec9706 Esophageal Cancer Cells