Initiation of Deoxyribonucleic Acid Synthesis After Thymine Starvation of
            <i>Bacillus subtilis</i>

Kallenbach, Neville R.; Ma, Rong-Ine

doi:10.1128/jb.95.2.304-309.1968

Cited by 9 publications

(5 citation statements)

References 25 publications

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“…In summary, the results support the existence of a regulatory mechanism in bacterial cells with the following properties: a fixed amount of an initiator substance must be synthesized for the induction of a round of chromosome replication and division follows C + D min later. The accumulation-like behavior of the initiation process offers a common explanation of the reports of an increased rate of DNA synthesis following restoration of DNA replication after a period of selective inhibition by thymine starvation (Nakada, 1960;Maaloe and Rasmussen, 1963;Pritchard and Lark, 1964;Kallenbach and Ma, 1968), ultraviolet light (Swenson and Setlow, 1966), or by other agents (Boyle et al, 1967). It accounts for and interprets the synchronous division observed by Barner and Cohen (1956).…”

mentioning

confidence: 62%

“…Pritchard and Lark (1964) suggested that thymine starvation initiated a new cycle of replication but only at the origin of one of the two partial replicas. Recently, Kallenbach and Ma (1968) reported enhanced initiation on only one of the arms of the replicating chromosome after 30 min of thymine starvation in Bacillus subtilis. These observations could also be consequences of symmetrical initiation at both origins in half of the cells in the population.…”

Section: Discussionmentioning

confidence: 99%

“…These observations could also be consequences of symmetrical initiation at both origins in half of the cells in the population. Since in the experiments of Kallenbach and Ma (1968), the generation time was 40-50 min, less than 70 % of the cells would be expected to initiate replication after 30 min of thymine starvation.…”

Section: Discussionmentioning

confidence: 99%

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Regulation of Cell Division in Escherichia coli

Pierucci¹

1969

Biophysical Journal

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The rate of cell division was measured in cultures of Escherichia coli B/r strain after periods of partial or complete inhibition of deoxyribonucleic acid (DNA) synthesis. The rate of DNA synthesis was temporarily decreased by removing thymidine from the growth medium or replacing it with 5-bromouracil. After restoration of DNA synthesis, a temporary period of accelerated cell division was observed. The results were consistent with the idea that chromosome replication begins when an initiator complement of fixed size accumulated in the cell. The increase in the potential for the initiation of new replication points during inhibition of DNA synthesis results in an increase in the rate of cell division after an interval which encompasses the time for the arrival of these replication points to the termini of the chromosomes and the time from this event to division.

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confidence: 62%

Section: Discussionmentioning

confidence: 99%

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Regulation of Cell Division in Escherichia coli

Pierucci¹

1969

Biophysical Journal

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“…Temporary selective blockage of chromosome replication apparently leads to premature chromosome reinitiation. Thus thymine starvation (11,14), nalidixic acid (6, 19), and temperature shift in thermosensitive DNA mutants (17,20) result in chromosome reinitiation from the origin prior to completion of the current round of chromosome replication. UVinduced DNA synthesis inhibition apparently has a similar effect.…”

Section: Discussionmentioning

confidence: 99%

Gene Frequency Analysis of Chromosomal Initiation Sites in Bacillus subtilis after Ultraviolet Light or X-Ray Exposure

1972

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Bacillus subtilis was exposed to ultraviolet light (UV) or X rays, and gene frequency analysis was used to study the location of initiation sites of postirradiation deoxyribonucleic acid (DNA) synthesis. It was found that DNA synthesis resumes primarily from the origin after UV exposure. With X irradiation, the origin is not selectively replicated. Elevated origin-to-terminal marker ratios observed after UV exposure of exponentially growing cells were interpreted as evidence for selective UV resistance of the replicative origin region of the bacterial chromosome. This laboratory has been engaged in studies aimed at defining the quantitative and regulatory aspects of chromosome replication after ultraviolet light (UV) or X-ray challenge to bacterial cells. By using a combination of density and radioisotopic labeling techniques, it was previously shown (4) that, when bulk deoxyribonucleic acid (DNA) synthesis re

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“…Recipient cells were grown overnight at 37 C on Tryptose Blood Agar Base (Difco) slants, washed once, and resuspended in Minimal A medium (4), Spizizen's minimal medium supplemented with 0.5% glucose, 0.05% Casamino Acids, and required nutritional components, all of which were added at a concentration of 50 ,ug/ml except adenine, which was added at 100 Ag/ml. Preparation of DNA from stationary-phase donor cultures was carried out as described previously (15), and the concentration was determined from its absorbance at 260 nm (assuming 1 A260 to be equivalent to 50 jug of DNA per ml) or by the indole test (17).…”

Section: Methodsmentioning

confidence: 99%

Intercellular Effects on Development of Competence in Bacillus subtilis

Goldsmith

Havas

et al. 1970

J Bacteriol

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The intercellular transfer of competence during growth under the conditions specified by the transformation procedure of Spizizen was investigated with Bacillus subtilis 168. The rate of competence development as assayed uniformly in medium B was not affected by variations in the cell concentration, although the first appearance of transformants occurred earlier with high cell densities in medium A, approximately in proportion to the onset of the stationary phase in the culture. Growth in the presence of Pronase enhanced the frequency of transformation, but did not detectably alter the kinetics of competence development. The rate of competence increase in physiologically noncompetent cultures was not changed by mixing with competent cultures either in medium A or in medium B; however, an early appearance of transformants was noted in mixed cultures in which the proportion of competent to noncompetent cells prevented exponential growth of the noncompetent strain. These experiments indicate that the normal development of competence in B. subtilis is not mediated by a soluble or loosely bound protein factor capable of transmitting competence directly via cell contact. The onset of competence is thus a function of internal physiological changes which are induced by the overall metabolic state of the culture.on July 31, 2020 by guest

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Initiation of Deoxyribonucleic Acid Synthesis After Thymine Starvation of Bacillus subtilis

Cited by 9 publications

References 25 publications

Regulation of Cell Division in Escherichia coli

Regulation of Cell Division in Escherichia coli

Gene Frequency Analysis of Chromosomal Initiation Sites in Bacillus subtilis after Ultraviolet Light or X-Ray Exposure

Intercellular Effects on Development of Competence in Bacillus subtilis

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