Injectable, Biodegradable Hydrogels for Tissue Engineering Applications

Tan, Huaping; Marra, Kacey G.

doi:10.3390/ma3031746

Cited by 571 publications

(420 citation statements)

References 145 publications

(208 reference statements)

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“…2,3,44 HA is a natural, negatively charged glycosaminoglycan that is widely distributed within the LP. HA plays a significant role in important biological processes such as tissue hydration, nutrient diffusion, proteoglycan organization, and cell differentiation, as well as in the tissue viscoelastic properties.…”

Section: Biomaterials Selection and Optimizationmentioning

confidence: 99%

A Flow Perfusion Bioreactor System for Vocal Fold Tissue Engineering Applications

Latifi

Heris

Thomson

et al. 2016

Tissue Engineering Part C: Methods

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The human vocal folds (VFs) undergo complex biomechanical stimulation during phonation. The aim of the present study was to develop and validate a phono-mimetic VF flow perfusion bioreactor, which mimics the mechanical microenvironment of the human VFs in vitro. The bioreactor uses airflow-induced self-oscillations, which have been shown to produce mechanical loading and contact forces that are representative of human phonation. The bioreactor consisted of two synthetic VF replicas within a silicone body. A cell-scaffold mixture (CSM) consisting of human VF fibroblasts, hyaluronic acid, gelatin, and a polyethylene glycol cross-linker was injected into cavities within the replicas. Cell culture medium (CCM) was perfused through the scaffold by using a customized secondary flow loop. After the injection, the bioreactor was operated with no stimulation over a 3-day period to allow for cell adaptation. Phonation was subsequently induced by using a variable speed centrifugal blower for 2 h each day over a period of 4 days. A similar bioreactor without biomechanical stimulation was used as the nonphonatory control. The CSM was harvested from both VF replicas 7 days after the injection. The results confirmed that the phono-mimetic bioreactor supports cell viability and extracellular matrix proteins synthesis, as expected. Many scaffold materials were found to degrade because of challenges from phonation-induced biomechanical stimulation as well as due to biochemical reactions with the CCM. The bioreactor concept enables future investigations of the effects of different phonatory characteristics, that is, voice regimes, on the behavior of the human VF cells. It will also help study the long-term functional outcomes of the VF-specific biomaterials before animal and clinical studies.

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Section: Biomaterials Selection and Optimizationmentioning

confidence: 99%

A Flow Perfusion Bioreactor System for Vocal Fold Tissue Engineering Applications

Latifi

Heris

Thomson

et al. 2016

Tissue Engineering Part C: Methods

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“…Hydrogels have a structure that is similar to the extracellular matrix (ECM) of many tissues, which will help with the cell integration [1,6,7]. Hydrogels also have the ability to homogenously encapsulate cells [1][2][3][4][5][6].…”

Section: Methodsmentioning

confidence: 99%

“…In the United States 18 people die daily waiting for an organ and more than 117,000 men, women and children await a life-saving organ transplant. Recently, 3D printing techniques are being employed to create complex biological structures with the long term goal of organ construction [1][2][3][4][5]. In this process, cells need to be seeded and cultured on printed scaffolds to generate tissue in a subsequent step.…”

Section: Introductionmentioning

confidence: 99%

Study of Tissue Printing Parameters for Generating Complex Tissue Constructs

Navarro¹,

García²,

Sundaram³

et al. 2016

J Tissue Sci Eng

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A mixture of agarose, MEM IX and HeLa cells (dubbed Bio-Ink) was created to allow normal cell interaction with the scaffold material (agarose) before crosslinking as an initial step in 3D printing tissue. Bio-Ink was developed successfully as an in situ-scaffolding material for engineering biological structures. Bio-Ink has been further conditioned by adjusting agarose composition and gelling time to obtain optimal HeLa cell growth. After detailed study, the time range available for printing this material, before full crosslinking occurs, was determined to be about 300 s, giving it attractive properties for 3D printing. Repeatable 10 mm thick prints were successful, although more system calibration is still needed to achieve more complex prints.

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“…PEG hydrogel degradation occurs by means of hydrolysis of sterile connections [5,6,8]. This process is initiated by the action of some nonspecific enzymes released by macrophages and fibroblasts of the tissue circumjacent to the hydrogel, like esterases, oxidases and reductases, which are eliminated through the lymphatic system.…”

Section: Photographic Assessmentmentioning

confidence: 99%

“…Reticulate PEG hydrogels are hydrophilic polymeric networks that absorb over 1,000 times its dry weight in water and are employed to restore soft tissues (tissue engineering), for presenting similarities with these tissues physical properties [6][7][8]. It is biocompatible [5], stable and degraded by hydrolysis of ester bonds, depending on the temperature and pH [5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Injectable Polyethylene Glycol Gel as Dermal Filler: 01 Year Clinical and Ultrasound Follow-Up

Addor¹,

Neri²

2016

J Clin Exp Dermatol Res

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Facial alterations that occur with aging are result of combined effects of soft tissue atrophy with loss of facial volume, gravitational factor and decrease of elastic tissue. In order to minimize these alterations, minimally invasive procedures such as cutaneous filling have been gaining space in clinical practice. In 2008, Scientech CorporationItalia introduced hydrogel of polyethylene glycol (PEG) REMAKE ® as cutaneous filler. This prospective study was developed to prove the effectiveness, safety and durability over the 12 months after the application of PEG hydrogel, commercialized under the name of REMAKE ® . 40 female volunteers were included, between 30 and 60 years, with signs of malar region volume loss between 2 and 3 (Raspaldo's classification) and Nasogenian furrow accentuation, with score between 2 and 3 (Day and collaborators' classification). No patient had carried out procedures of temporary filling for 1 year or permanent filling at any time. Objective parameters always show the scale of the improvement and an evaluation was selected through ultrasound exams and surface scan for evaluation of size and depth of wrinkles improvement. Evaluations by the US were carried out for 30, 120 and 360 days of product use, in a pilot group of 18 patients randomly selected to measure the product in the application plan and numerically assess alterations of volume (absorption/migration). Its durability of up to 01 year after application is proved when no significant reduction.

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Injectable, Biodegradable Hydrogels for Tissue Engineering Applications

Cited by 571 publications

References 145 publications

A Flow Perfusion Bioreactor System for Vocal Fold Tissue Engineering Applications

A Flow Perfusion Bioreactor System for Vocal Fold Tissue Engineering Applications

Study of Tissue Printing Parameters for Generating Complex Tissue Constructs

Injectable Polyethylene Glycol Gel as Dermal Filler: 01 Year Clinical and Ultrasound Follow-Up

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