Injectable Cartilage

Dobratz, Eric J.; Kim, Soo Whan; Voglewede, Andrew; Park, Stephen S.

doi:10.1001/archfacial.2008.509

Cited by 50 publications

(11 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Research is ongoing to find an injectable cell/polymer solution that forms a construct with robust mechanical properties in the defect site [315][316][317]. If implanted immediately, the scaffold should possess mechanical characteristics that are appropriate for the loading environment.The scaffold should maintain its shape and protect the seeded cells from any excessive forces.…”

Section: Scaffold Designmentioning

confidence: 99%

Articular Cartilage Tissue Engineering

Athanasiou¹,

Darling²,

Hu³

2009

Synthesis Lectures on Tissue Engineering

184

View full text Add to dashboard Cite

Section: Scaffold Designmentioning

confidence: 99%

Articular Cartilage Tissue Engineering

Athanasiou¹,

Darling²,

Hu³

2009

Synthesis Lectures on Tissue Engineering

184

View full text Add to dashboard Cite

“…These biochemical data are consistent with the development of new cartilage. 38 Finally, fluorescent measurements were used to ascertain the viability of the 3D printed bionic ear tissue after 10 weeks of in vitro growth culture using fluorescein diacetate (FDA) and propidium iodide (PI) stains. Figures 2G and 2H show the tissue covering the coil antenna and the internal tissue that is in contact with the electrode that runs perpendicular through the tissue, respectively.…”

mentioning

confidence: 99%

3D Printed Bionic Ears

et al. 2013

View full text Add to dashboard Cite

The ability to three-dimensionally interweave biological tissue with functional electronics could enable the creation of bionic organs possessing enhanced functionalities over their human counterparts. Conventional electronic devices are inherently two-dimensional, preventing seamless multidimensional integration with synthetic biology, as the processes and materials are very different. Here, we present a novel strategy for overcoming these difficulties via additive manufacturing of biological cells with structural and nanoparticle derived electronic elements. As a proof of concept, we generated a bionic ear via 3D printing of a cell-seeded hydrogel matrix in the precise anatomic geometry of a human ear, along with an intertwined conducting polymer consisting of infused silver nanoparticles. This allowed for in vitro culturing of cartilage tissue around an inductive coil antenna in the ear, which subsequently enables readout of inductively-coupled signals from cochlea-shaped electrodes. The printed ear exhibits enhanced auditory sensing for radio frequency reception, and complementary left and right ears can listen to stereo audio music. Overall, our approach suggests a means to intricately merge biologic and nanoelectronic functionalities via 3D printing.

show abstract

“…sodium alginate), which is a type of anionic polysaccharide without a side chain derived from brown algae, exists as a solid when it interacts with polyvalent cations (e.g. calcium) (1,16). Alginate displays outstanding biocompatibility with the host and seed cells in condition of maintaining their own invariant characteristics (1).…”

Section: Discussionmentioning

confidence: 99%

Bone marrow mesenchymal stem cells combined with calcium alginate gel modified by hTGF-β1 for the construction of tissue-engineered cartilage in three-dimensional conditions

Zhu

Zhang

Sun

et al. 2012

Experimental and Therapeutic Medicine

View full text Add to dashboard Cite

The aim of this study was to investigate the feasibility of Ad-hTGF-β1-transfected bone marrow mesenchymal stem cells (BMMSCs) combined with calcium alginate gel for the construction of tissue-engineered cartilage under three-dimensional conditions. Rat BMMSCs were divided into three groups: the Ad-hTGF-β1 transfection group, the Ad-EGFP transfection group and the control group. The BMMSCs in the Ad-hTGF-β1 transfection group were continually grown. The compound of cell-calcium alginate gel was cultured in a constant temperature incubator. The morphology of cells was examined, and the proliferation of cells was detected by MTT assay. The results from real-time PCR showed that the average relative ratio of TGF-β1 and transcriptional coactivator with PDZ-binding motif (TAZ) in the Ad-hTGF-β1 group was comparable to that of the control group (P<0.05). Using western blotting and immunohistochemistry, strong expression of collagen II in the Ad-hTGF-β1 group was detected. The results from western blotting showed that the expression of TGF-β1 in the Ad-hTGF-β1 group was significantly increased compared with that of the other two groups. The differentiation of BMMSCs was induced by Ad-hTGF-β1 transfection into chondrocytes. TGF-β1 may promote the differentiation of BMMSCs into chondrocytes by TAZ. BMMSCs transfected by Ad-hTGF-β1 could be induced into chondrocytes. These three-dimensional conditions could preferably mimic cell growth patterns in vivo.

show abstract

Injectable Cartilage

Cited by 50 publications

References 16 publications

Articular Cartilage Tissue Engineering

Articular Cartilage Tissue Engineering

3D Printed Bionic Ears

Bone marrow mesenchymal stem cells combined with calcium alginate gel modified by hTGF-β1 for the construction of tissue-engineered cartilage in three-dimensional conditions

Contact Info

Product

Resources

About