Injectable, high‐density collagen gels for annulus fibrosus repair: An<i>in vitro</i>rat tail model

Borde, Brandon; Grunert, Peter; Härtl, Roger; Bonassar, Lawrence J.

doi:10.1002/jbm.a.35388

Cited by 58 publications

(44 citation statements)

References 56 publications

(115 reference statements)

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“…Finally, within each group, the rats were assigned to 4, 8, and 12 weeks postoperative time points of sacrifice (each; n = 9). A rat tail AF injury model was created according to methods of Borde et al and Kazezian et al with minor modifications . Surgery was performed under general anesthesia using 2.5% isoflurane inhalation while in a prone position.…”

Section: Methodsmentioning

confidence: 99%

Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model

et al. 2019

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In recent years, studies have explored novel approaches for cell transplantation to enable annulus fibrosus (AF) regeneration of the intervertebral disc in particular for lumbar disc herniation. Nevertheless, successful engraftment of cells is structurally challenging, and no definitive method has yet been established. This study investigated the potential of cell sheet technology to facilitate cell engraftment for AF repair. AF injury was induced by a 1 × 1 mm defect in rat tails after which AF cell sheets were transplanted. Its regenerative effects were compared to a nondegenerated and degeneration only conditions. Degenerative changes of the entire intervertebral disc were examined by disc height measurements, histology, and immunohistochemistry for 4‐, 8‐, and 12‐weeks post‐transplantation. Cell engraftment was confirmed by tracing PKH26 fluorescent dyed AF cells. In the transplant group, disc degeneration was significantly suppressed after 4, 8, and 12 weeks when compared with the degenerative group, as indicated by histological scoring and DHI observations. At 2 and 4 weeks after transplant, PKH26 positive cells could be detected in defect region and surrounding AF. The results suggest cell engraftment into AF tissue could be established by the cell sheet technology without additional scaffolding or adhesives. In short, AF cell sheets appear to be an effective and accessible tool for AF repair and to support intervertebral disc regeneration.

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Section: Methodsmentioning

confidence: 99%

Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model

et al. 2019

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Section: Methodsmentioning

confidence: 99%

“…We adapted the cell preparation methods from previously established protocols. 8,9 Briefly, we harvested cervical IVDs from three skeletally mature canine spines (18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36) Similarly, we based the TE-IVD fabrication process on established techniques. 9,15 First, we mixed encapsulated canine NP cells (25 × 10 6 cells/mL) in 3% wt./vol.…”

Section: Cell Isolation and Te-ivd Fabricationmentioning

confidence: 99%

“…For the first protocol, we clamped the caudal VB portion of the specimen to the load cell on a mechanical testing system (ELF 3200, EnduraTech, Eden Prairie, MN), while an impermeable plate applied 5% compressive strain steps up to 15% strain on the cranial VB portion ( Figure 1E). 9,23 During each of the intact and experimental conditions described above, we kept the specimens surrounded by a gauze soaked with PBS (MediaTech) containing protease inhibitors (Roche Diagnostics, Indianapolis, IN). From the resulting load-displacement data, we calculated an effective stiffness for the motion segment in equilibrium, and data from DX, PLATE−, and PLATE+ groups were normalized against their corresponding CTRL segments to calculate ratio of stiffness to intact segments under each condition.…”

Section: Biomechanical Testingmentioning

confidence: 99%

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Resorbable plating system stabilizes tissue‐engineered intervertebral discs implanted ex vivo in canine cervical spines

et al. 2018

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Total disc replacement using tissue‐engineered intervertebral discs (TE‐IVDs) may offer a biological alternative to treat radiculopathy caused by disc degeneration. A composite TE‐IVD was previously developed and evaluated in rat tail and beagle cervical spine models in vivo. Although cell viability and tissue integration into host tissue were promising, significant implant displacement occurred at multiple spinal levels. The goal of the present study was to assess the effects of a resorbable plating system on the stiffness of motion segments and stability of tissue‐engineered implants subjected to axial compression. Canine motion segments from levels C2/C3 to C5/C6 were assessed as intact (CTRL), after discectomy (Dx), with an implanted TE‐IVD only (PLATE−), and with a TE‐IVD combined with an attached resorbable plate (PLATE+). Segments under PLATE+ conditions fully restored separation between endplates and showed significantly higher compressive stiffness than segments under PLATE− conditions. Plated segments partially restored more than 25% of the CTRL motion segment stiffness. Plate attachment also prevented implant extrusion from the disc space at 50% compressive strain, and this effect was more significant in segments from levels C3/C4 when compared to segments from level C5/C6. These results suggest that stabilization of motion segments via resorbable plating assists TE‐IVD retention in the disc space while allowing the opportunity for implants to fully integrate into the host tissue and achieve optimal restoration of spine biomechanics.

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“…It is most frequent used as hard and soft capsules, microspheres, sealants for vascular prostheses, wound dressing, drug delivery agent and three-dimensional tissue scaffold in tissue engineering 11,12,14,15 Collagen is used in tissue engineering as scaffolds in three forms. The first form is the collagen gel system 16,17 . The second form of collagen scaffold is the nanofiber sheets 18,19 .…”

Section: Introductionmentioning

confidence: 99%

Extraction and Characterization of Collagen from Buffalo Skin for Biomedical Applications

Rizk¹,

Mostafa²

2016

Orient. J. Chem

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Collagen is widely used for biomedical and pharmaceutical applications due to its excellent biocompatibility, biodegradability and weak antigenicity. However, applicability is limited due to its high cost and probability of disease transmission from the current sources, which are bovine and porcine. In the present study, collagen was extracted from 6 months buffalo skins as alternative save sources. Collagen was characterized by different physico-chemical techniques like ATR-FTIR, Raman, SEM, DSC and amino acids analysis. Proline and hydroxyproline contents of buffalo skin collagen were higher than those of calf skin collagen. Thermal stability of buffalo skin collagen is high with respect to that of calf skin collagen. The obtained buffalo skin collagen shows higher stiffness upon cross-linking with glutaraldehyde. Thus buffalo skin collagen can be used for fabrication of high strength bioactive sponge and sheets for medical applications, like scaffold for tissue engineering, drug delivery and wound dressing system.

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Injectable, high‐density collagen gels for annulus fibrosus repair: Anin vitrorat tail model

Cited by 58 publications

References 56 publications

Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model

Annulus fibrosus cell sheets limit disc degeneration in a rat annulus fibrosus injury model

Resorbable plating system stabilizes tissue‐engineered intervertebral discs implanted ex vivo in canine cervical spines

Extraction and Characterization of Collagen from Buffalo Skin for Biomedical Applications

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