Injectable hydrogel based on dialdehyde galactomannan and N-succinyl chitosan: a suitable platform for cell culture

Lima, Everton Lucas de; Vasconcelos, Niédja Fittipaldi; Maciel, Jeanny da Silva; Andrade, Fábia Karine; Vieira, Rodrigo Silveira; Feitosa, Judith P.A.

doi:10.1007/s10856-019-6343-6

Cited by 24 publications

(10 citation statements)

References 35 publications

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“…Taking the aforementioned limitations into account, it was necessary to explore a stable, effective, and simple COH animal model and to conduct a comprehensive evaluation. The cross-linking hydrogel has low toxicity, high water-retention capacity, multifunctional usefulness, and excellent biocompatibility [ 35 , 36 ], and has been applied in several biomedical research fields such as drug release [ 37 , 38 ], cell culture [ 39 ], tissue regeneration [ 40 – 42 ], 3D bioprinting [ 43 ], and prevention of postoperative intra-abdominal adhesion [ 44 ]. In the field of ophthalmology, cross-linking hydrogel has been used in tissue engineering, including corneal transplant [ 45 , 46 ] and retinal progenitor cell transplantation [ 47 ], drug delivery systems for multiple eye diseases such as glaucoma [ 48 ] and uveitis treatment [ 49 ], treatment of ocular surface [ 50 ] and lacrimal diseases [ 51 ], and substitution of the vitreous body [ 52 , 53 ], showing great potential.…”

Section: Discussionmentioning

confidence: 99%

Efficacy, Drug Sensitivity, and Safety of a Chronic Ocular Hypertension Rat Model Established Using a Single Intracameral Injection of Hydrogel into the Anterior Chamber

Huang

Chen

et al. 2020

Med Sci Monit

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Section: Discussionmentioning

confidence: 99%

Efficacy, Drug Sensitivity, and Safety of a Chronic Ocular Hypertension Rat Model Established Using a Single Intracameral Injection of Hydrogel into the Anterior Chamber

Huang

Chen

et al. 2020

Med Sci Monit

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“…The aldehyde groups introduced into the polysaccharide structure can serve as cross-linking agents for biopolymers. The dialdehyde polysaccharides based on pectin [24], chitosan [25], xylan [26], alginate [27], starch [28], hyaluronic acid [29], pullulan [30], galactomannan [31], cellulose [32], carboxymethyl cellulose [33], and xanthan gum [34] are used for cross-linking of polymers containing amino groups. Proteins play a crucial role in the functioning of living cells.…”

Section: Introductionmentioning

confidence: 99%

Dialdehyde Starch Nanocrystals as a Novel Cross-Linker for Biomaterials Able to Interact with Human Serum Proteins

Węgrzynowska-Drzymalska

Mylkie

Nowak

et al. 2022

IJMS

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In recent years, new cross-linkers from renewable resources have been sought to replace toxic synthetic compounds of this type. One of the most popular synthetic cross-linking agents used for biomedical applications is glutaraldehyde. However, the unreacted cross-linker can be released from the materials and cause cytotoxic effects. In the present work, dialdehyde starch nanocrystals (NDASs) were obtained from this polysaccharide nanocrystal form as an alternative to commonly used cross-linking agents. Then, 5−15% NDASs were used for chemical cross-linking of native chitosan (CS), gelatin (Gel), and a mixture of these two biopolymers (CS-Gel) via Schiff base reaction. The obtained materials, forming thin films, were characterized by ATR-FTIR, SEM, and XRD analysis. Thermal and mechanical properties were determined by TGA analysis and tensile testing. Moreover, all cross-linked biopolymers were also characterized by hydrophilic character, swelling ability, and protein absorption. The toxicity of obtained materials was tested using the Microtox test. Dialdehyde starch nanocrystals appear as a beneficial plant-derived cross-linking agent that allows obtaining cross-linked biopolymer materials with properties desirable for biomedical applications.

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“… 40 In addition, nano-ATP has a special nanoscale structure in which nano-rods can form a high-viscosity network structure resulting from the interactions among rods, which may promote osteogenesis when being doped in composite scaffolds according to the previous reports. 28 , 41 Nano-ATP can also form a stable blend when combined with other polymer chains. These features render nano-ATP an appropriate material for osteoblast ingrowth.…”

Section: Introductionmentioning

confidence: 99%

3D Printed Gelatin/Sodium Alginate Hydrogel Scaffolds Doped with Nano-Attapulgite for Bone Tissue Repair

Liu¹,

Qin²,

Wang³

et al. 2021

IJN

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Introduction Bone tissue engineering (BTE) is a new strategy for bone defect repair, but the difficulties in the fabrication of scaffolds with personalized structures still limited their clinical applications. The rapid development in three-dimensional (3D) printing endows it capable of controlling the porous structures of scaffolds with high structural complexity and provides flexibility to meet specific needs of bone repair. Methods In this study, sodium alginate (SA)/gelatin (Gel) hydrogel scaffolds doped with different contents of nano-attapulgite were fabricated via 3D printing. The surface microstructure, hydrophilicity and mechanical properties were fully evaluated. Furthermore, mouse bone marrow-derived mesenchymal stem cells (BMSCs) were cultured with the composite hydrogels in vitro, and proliferation and osteoblastic differentiation were assessed. A rabbit tibia plateau defect model was used to evaluate the osteogenic potential of the composite hydrogel in vivo. Results When increasing nano-ATP content, the Gel/SA/nano-ATP composite hydrogels showed better mechanical property and printability. Moreover, Gel/SA/nano-ATP composite hydrogels showed excellent bioactivity, and a significant mineralization effect was observed on the surface after being incubated in simulated body fluid (SBF) for 14 days. The Gel/SA/nano-ATP composite hydrogel also showed good biocompatibility and promoted the osteogenesis of BMSCs. Finally, histological analysis demonstrates that the Gel/SA/nano-ATP composite hydrogels could effectively enhance bone regeneration in vivo. Conclusion These properties render the Gel/SA/nano-ATP composite hydrogel scaffolds an ideal bone tissue engineering material for the repair of bone defects.

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Injectable hydrogel based on dialdehyde galactomannan and N-succinyl chitosan: a suitable platform for cell culture

Cited by 24 publications

References 35 publications

Efficacy, Drug Sensitivity, and Safety of a Chronic Ocular Hypertension Rat Model Established Using a Single Intracameral Injection of Hydrogel into the Anterior Chamber

Efficacy, Drug Sensitivity, and Safety of a Chronic Ocular Hypertension Rat Model Established Using a Single Intracameral Injection of Hydrogel into the Anterior Chamber

Dialdehyde Starch Nanocrystals as a Novel Cross-Linker for Biomaterials Able to Interact with Human Serum Proteins

3D Printed Gelatin/Sodium Alginate Hydrogel Scaffolds Doped with Nano-Attapulgite for Bone Tissue Repair

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