Injectable hydrogel induces regeneration of naturally degenerate human intervertebral discs in a loaded organ culture model

Cherif, Hosni; Li, Li; Snuggs, Joseph; Li, Xuan; Sammon, Christopher; Li, Jianyu; Beckman, Lorne; Haglund, Lisbet; Le Maitre, Christine. L．

doi:10.1016/j.actbio.2023.12.041

Cited by 3 publications

(2 citation statements)

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“…Preclinical evaluations could utilize ex vivo human IVDs in bioreactors to assess long-term fatigue performance and regenerative outcomes under physiological loading conditions. 56 Additionally, animal experiments are crucial for evaluating in vivo repair performance and potential foreignbody responses. Established animal models, such as rat tail IVD and goat cervical disc models, have been employed in previous studies to assess the safety and efficacy of various implants.…”

Section: Discussionmentioning

confidence: 99%

“…While our study showcases the design and application of composite hydrogel sealants for annulus fibrosus repair, further validation through in vivo and ex vivo studies is needed to fully ascertain their regenerative capabilities and clinical effectiveness. Preclinical evaluations could utilize ex vivo human IVDs in bioreactors to assess long-term fatigue performance and regenerative outcomes under physiological loading conditions . Additionally, animal experiments are crucial for evaluating in vivo repair performance and potential foreign-body responses.…”

Section: Discussionmentioning

confidence: 99%

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Composite Hydrogel Sealants for Annulus Fibrosus Repair

Li,

Huo,

et al. 2024

ACS Biomater. Sci. Eng.

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Intervertebral disc (IVD) herniation is a leading cause of disability and lower back pain, causing enormous socioeconomic burdens. The standard of care for disc herniation is nucleotomy, which alleviates pain but does not repair the annulus fibrosus (AF) defect nor recover the biomechanical function of the disc. Existing bioadhesives for AF repair are limited by insufficient adhesion and significant mechanical and geometrical mismatch with the AF tissue, resulting in the recurrence of protrusion or detachment of bioadhesives. Here, we report a composite hydrogel sealant constructed from a composite of a three-dimensional (3D)-printed thermoplastic polyurethane (TPU) mesh and tough hydrogel. We tailored the fiber angle and volume fraction of the TPU mesh design to match the angle-ply structure and mechanical properties of native AF. Also, we proposed and tested three types of geometrical design of the composite hydrogel sealant to match the defect shape and size. Our results show that the sealant could mimic native AF in terms of the elastic modulus, flexural modulus, and fracture toughness and form strong adhesion with the human AF tissue. The bovine IVD tests show the effectiveness of the composite hydrogel sealant for AF repair and biomechanics recovery and for preventing herniation with its heightened stiffness and superior adhesion. By harnessing the combined capabilities of 3D printing and bioadhesives, these composite hydrogel sealants demonstrate promising potential for diverse applications in tissue repair and regeneration.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%