Injectable hydrogels for cartilage tissue engineering

Jin, Rong

doi:10.3990/1.9789036529204

Cited by 6 publications

(6 citation statements)

References 101 publications

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“…The transmission of ultraviolet (100-400 nm), visible (400-800 nm), and infrared (800-1,000 nm) radiation through articular cartilage is a function of the optical properties of the tissue's cellular and extracellular matrix. Mature articular cartilage has four unique zones which define the microstructural organization (Erickson et al, 2009;Jin et al, 2009;Lee et al, 2001) There are three wavelength ranges for ultraviolet (UV) light radiation, UVC (short) 180-280 nm, UVB (medium) 280-320 nm, and UVA (long) 320-400 nm. The most commonly used UV light wavelength is UVA 365 nm (long), which is the least cytotoxic and deeper penetrating wavelength (Bryant, Nuttelman, & Anseth, 2000;Maloney et al, 2006;Williams, Malik, Kim, Manson, & Elisseeff, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Other approaches use cells embedded in polymer hydrogels formed from such biocompatible materials as hyaluronic acid, chondroitin sulfate, soluble collagen, poly(ethylene glycol), poly(vinyl alcohol), poly(propylene fumarate), alginate, and even agarose (Kim, Mauck, & Burdick, 2011;Luyten & Vanlauwe, 2012). Typical methods for hydrogel formation involve cross-linking the polymer by photo-oxidation (e.g., ultraviolet or UV light enhanced with a macromer and photo-initiator), chemical or redox (reductionoxidation reaction), and heating (Bakos, Jorge-Herrero & Koller, 2000;Bryant, Chowdhury, Lee, Bader, & Anseth, 2004;Erickson et al, 2009;Jin et al, 2009;Koob et al, 2000;Lee, Grodzinsky, & Spector, 2001;Li et al, 2004;Sharma et al, 2013).…”

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confidence: 99%

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Ultraviolet light (365 nm) transmission properties of articular cartilage as a function of depth, extracellular matrix, and swelling

Torzilli

Azimulla

2019

J Biomedical Materials Res

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Current tissue engineering approaches for treatment of injured or diseased articular cartilage use ultraviolet light (UV) for in situ photopolymerization of biomaterials to fill chondral and osteochondral defects as well as resurfacing, stiffening and bonding the extracellular matrix and tissue interfaces. The most commonly used UV light wavelength is UVA 365 nm, the least cytotoxic and deepest penetrating. However, little information is available on the transmission of UVA 365 nm light through the cartilage matrix. In the present study, 365 nm UV light transmission was measured as a function of depth through 100 μm thick slices of healthy articular cartilage removed from mature bovine knees. Transmission properties were measured in normal (Native) cartilage and after swelling equilibration in phosphate-buffered saline (Swollen). Single-factor and multiple linear regression analyses were performed to determine depth-dependencies between the effective attenuation coefficients and proteoglycan, collagen and water contents. For both cartilages, a significant depthdependency was found for the effective attenuation coefficients, being highest at the articular surface (superficial zone) and decreasing with depth. The effective attenuation coefficients for full-thickness cartilages were approximately a third lower than the total attenuation coefficients calculated from the individual slices. Analysis of absorption and scattering effects due to the ECM and chondrocytes found that UV light scatter coefficients were 10 times greater than absorption coefficients. The greater transmittance of UV light through the thicker cartilage was attributed to the collagen within the ECM causing significant backscatter forward reflectance. K E Y W O R D S cartilage, depth-dependent, forward backscatter reflectance, optical properties, ultraviolet light

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Ultraviolet light (365 nm) transmission properties of articular cartilage as a function of depth, extracellular matrix, and swelling

Torzilli

Azimulla

2019

J Biomedical Materials Res

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“…In one experiment using C6 glioma cells, it was dosclosed that the average cytotoxic concentration of H2O2 was reduced from 500-30 μM as the incubation time was raised from 1 to 24 h (155). This dependance indicates that the cytotoxic consequences of H2O2 could be less significant if H2O2 is quickly used by the HRP mediated oxidative reaction (119,123,156). Also, it was shown that the transient contact to H2O2 through the crosslinking procedure could help rat aNSCs to endure oxidative stress, showing a higher survival rate in comparison to the cells without pre-exposure when exposed to the second addition of H2O2 (119).…”

Section: Cytotoxicity Of H2o2mentioning

confidence: 99%

Enzymatic Crosslinked Hydrogels for Biomedical Application

Badali¹,

Mohajer²,

Hassanzadeh³

et al. 2021

Preprint

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Self-assembled structures mostly arises through enzyme-regulated phenomena in nature under persistent conditions. Enzymatic reactions are one of main biological processes in fabrication and construction of supramolecular hydrogel networks required for biomedical applications. The enzymatic processes provide a unique opportunity to integrate hydrogel formation. In most of cases, structure and substrates of hydrogels are adjusted by enzyme catalysis due to the chemo-, regio- and stereo-selectivity of enzymes. Hydrogels processed by using various enzyme schemes showed remarkable characteristics as dynamic frames for cells, bioactive molecules and drugs in biomedical applications. A novel class of enzyme-mediated crosslinking hydrogels mimics the extracellular matrices by displaying unique physicochemical properties and functionalities like water-retention capacity, drug loading ability, biodegradability, biocompatibility, biostability, bioactivity, optoelectronic properties, self-healing ability, shape memory ability. In recent years, many enzymatic systems investigated hydrogel cross-linking. Results of biocompatible hydrogel products show that these mechanisms of crosslinking can fulfill requirements for variety of biomedical applications including tissue engineering, wound healing and drug delivery.

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“…Wang and Stegemann developed crosslinked chitosan/collagen hydrogels for bone tissue engineering applications with a storage modulus of up to 0.35 kPa (Wang & Stegemann, 2011). Similarly, Jin et al (2009) developed an injectable chitosan-based scaffold with a storage modulus of up to 0.5 kPa in physiological conditions. Therefore, when comparing the present system to the systems existing in the literature, it is concluded that the freeze-drying system is a promising method to fabricate higher strength hydrated natural polymer networks.…”

Section: Chitosan Scaffolds For Bone Tissue Engineering Applicationsmentioning

confidence: 99%

Immobilization of nanocarriers within a porous chitosan scaffold for the sustained delivery of growth factors in bone tissue engineering applications

Witte

Wagner

Fratila‐Apachitei

et al. 2020

J Biomedical Materials Res

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To guide the natural bone regeneration process, bone tissue engineering strategies rely on the development of a scaffold architecture that mimics the extracellular matrix and incorporates important extracellular signaling molecules, which promote fracture healing and bone formation pathways. Incorporation of growth factors into particles embedded within the scaffold can offer both protection of protein bioactivity and a sustained release profile. In this work, a novel method to immobilize carrier nanoparticles within scaffold pores is proposed. A biodegradable, osteoconductive, porous chitosan scaffold was fabricated via the “freeze‐drying method,” leading to scaffolds with a storage modulus of 8.5 kPa and 300 μm pores, in line with existing bone scaffold properties. Next, poly(methyl methacrylate‐co‐methacrylic acid) nanoparticles were synthesized and immobilized to the scaffold via carbodiimide‐crosslinker chemistry. A fluorescent imaging study confirmed that the conventional methods of protein and nanocarrier incorporation into scaffolds can lead to over 60% diffusion out of the scaffold within the first 5 min of implantation, and total disappearance within 4 weeks. The novel method of nanocarrier immobilization to the scaffold backbone via carbodiimide‐crosslinker chemistry allows full retention of particles for up to 4 weeks within the scaffold bulk, with no negative effects on the viability and proliferation of human umbilical vein endothelial cells.

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Injectable hydrogels for cartilage tissue engineering

Cited by 6 publications

References 101 publications

Ultraviolet light (365 nm) transmission properties of articular cartilage as a function of depth, extracellular matrix, and swelling

Ultraviolet light (365 nm) transmission properties of articular cartilage as a function of depth, extracellular matrix, and swelling

Enzymatic Crosslinked Hydrogels for Biomedical Application

Immobilization of nanocarriers within a porous chitosan scaffold for the sustained delivery of growth factors in bone tissue engineering applications

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