Injectable pectin hydrogels produced by internal gelation: pH dependence of gelling and rheological properties

Moreira, Helena; Munarin, Fabiola; Gentilini, Roberta; Visai, Livia; Granja, Pedro L.; Tanzi, Maria Cristina; Petrini, Paola

doi:10.1016/j.carbpol.2013.12.057

Cited by 141 publications

(98 citation statements)

References 54 publications

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“…[25][26][27] However, the potential of pectin hydrogels crosslinked using this system for cell-delivery applications has not yet been explored. The internal gelation of pectin using CaCO 3 (without GDL) was previously explored, 28 showing how the internal gelation allows ne-tuning of the properties of the gels by an accurate control over the pH and the amount of CaCO 3 . However, until now, no cell embedding studies have been described for these gels.…”

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confidence: 99%

Biofunctionalized pectin hydrogels as 3D cellular microenvironments

et al. 2015

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In situ-forming hydrogels of pectin, a polysaccharide present in the cell wall of higher plants, were prepared using an internal ionotropic gelation strategy based on calcium carbonate/D-glucono-d-lactone, and explored for the first time as cell delivery vehicles. Since no ultrapure pectins are commercially available yet, a simple and efficient purification method was established, effectively reducing the levels of proteins, polyphenols and endotoxins of the raw pectin. The purified pectin was then functionalized by carbodiimide chemistry with a cell-adhesive peptide (RGD). Its gelation was analyzed by rheometry and optimized. Human mesenchymal stem cells embedded within unmodified and RGD-pectin hydrogels of different viscoelasticities (1.5 and 2.5 wt%) remained viable and metabolically active for up to 14 days. On unmodified pectin hydrogels, cells remained isolated and round-shaped. In contrast, within RGD-pectin hydrogels they elongated, spread, established cell-to-cell contacts, produced extracellular matrix, and migrated outwards the hydrogels. After 7 days of subcutaneous implantation in mice, acellular pectin hydrogels were considerably degraded, particularly the 1.5 wt% hydrogels. Altogether, these findings show the great potential of pectin-based hydrogels, which combine an interesting set of easily tunable properties, including the in vivo degradation profile, for tissue engineering and regenerative medicine.

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confidence: 99%

Biofunctionalized pectin hydrogels as 3D cellular microenvironments

et al. 2015

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“…The properties of pectin hydrogels are known to be easily tunable by varying the pH of pectin solutions [37] and the solubility of hydroxyapatite is strongly dependent on pH, thus modifying the amount of Ca 2+ available for the crosslinking. Hydrogel samples were produced at different pH, employing pectin at its native pH (pH 3.2) or adjusting the pH to 3.7.…”

Section: Preparation Of the Composite Hydrogels And Ph Variationsmentioning

confidence: 99%

“…Pectin can form gels in the presence of calcium ions [33][34][35][36][37][38]; therefore, hydroxyapatite was selected both as cross-linking agent and as reinforcement of the biocomposite. The limited water solubility of hydroxyapatite allows obtaining a certain amount of free calcium ions which can be involved in the gelling process while the remaining insoluble part can act as reinforcement of the biocomposite.…”

Section: Introductionmentioning

confidence: 99%

Micro- and nano-hydroxyapatite as active reinforcement for soft biocomposites

Munarin

Petrini

Gentilini

et al. 2015

International Journal of Biological Macromolecules

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“…Polysaccharide solutions and gels are generally characterized by a solid-like behaviour dominating over the liquid-like ones (G > G ) (Moreira et al, 2014). Fig.…”

Section: Elastic and Viscous Modulusmentioning

confidence: 99%