Injectable Shape-Holding Collagen Hydrogel for Cell Encapsulation and Delivery Cross-linked Using Thiol-Michael Addition Click Reaction

Pupkaite, Justina; Rosenquist, Jenny; Hilborn, Jöns; Samanta, Ayan

doi:10.1021/acs.biomac.9b00769

Cited by 78 publications

(67 citation statements)

References 68 publications

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“…These results, obtained using human cells, are as good as the results of studies which reported the great biocompatible hydrogels. [13,37] In this regard, our findings demonstrated that the hydrogel of this study is highly biocompatible in cell culture and expansion studies.…”

Section: Live/dead and Mtt Assaysupporting

confidence: 57%

Amylopectin Multiple Aldehyde Crosslinked Hydrogel as an Injectable and Self‐Healing Cell Carrier for Bone Tissue Engineering

Vahedi

Shokrolahi

Barzin

et al. 2020

Macro Materials & Eng

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linkages formed between aldehyde and amine functional groups offer unique properties including self-healing and injectability in the so-called dynamic hydrogels. [1][2][3][4][5] Hence, Schiff base-based dynamic hydrogels have found application in regenerative medicine, drug delivery, [6,7] wound dressing. [8,9] Cell delivery for regenerative medicine is one of the marvelous applications of dynamic hydrogels based on natural polymers which are addressed in recent scientific reports. For example, chitosan crosslinked using polyethylene glycol dibenzaldehyde through Schiff base linkages were found to be an injectable and self-healing hydrogel. Such prepared hydrogels have been used as a cell delivery vehicle for encapsulation of neural stem cells for promoting nerve regeneration. [3] Another self-healing/injectable hydrogel was reported as a cell carrier for cardiac cell therapy by Schiff base linkages between chitosan chains and polyethylene glycol dibenzaldehyde. [10] As another example, a self-healing and injectable hydrogel cell carrier for myoblast cell therapy was reported prepared by Schiff base linkages between N-carboxyethyl chitosan and dextran-graft-aniline tetramer-graft-4-formylbenzoic acid. [11] Such studies have been focused on the formation of Schiff base linkages via benzaldehyde derivatives for crosslinking chitosan as the amine sharing part and the obtained hydrogels support both injectability and self-healing characteristics as the cell delivery systems.On the other hand, there are some reports on fabrication of injectable and self-healing dynamic hydrogels based on Schiff base linkages by aliphatic aldehyde sharing parts for cell delivery applications. For example, an injectable and self-healing hydrogel was prepared via formation of Schiff base linkages between N-succinyl-chitosan and chondroitin sulfate multiple aldehyde. The reported hydrogel was used for encapsulation of Hela cells. [12] Also, a hydrogel with mentioned characteristics was reported which was fabricated by the formation of Schiff base linkages between N-carboxyethyl chitosan and oxidized sodium alginate and was used as a cell carrier for neural stem cells. [13] Moreover, another injectable and self-healing hydrogel Despite excellent processing and biological properties of gelatin for use as a cell carrier, none of the gelatin-based hydrogel cell carriers reported to date offer all characteristics including quick formation, injectability, self-healing, and durability, which are simultaneously required for an ideal system. Here, a gelatin-based hydrogel with dynamic Schiff base linkages, so-called "dynamic hydrogel," as an injectable cell carrier consisting of gelatin and amylopectin multiple aldehyde (AMPA), with all the required characteristics is reported. Biocompatibility and osteoinductivity of the hydrogel are verified through the culture of human bone marrow-derived mesenchymal stem cells (hBMSCs). As live/dead results show, hBMSCs are alive and highly viable ≈85-90% within the hydrogel after 5 days. According to bromodeo...

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Section: Live/dead and Mtt Assaysupporting

confidence: 57%

Amylopectin Multiple Aldehyde Crosslinked Hydrogel as an Injectable and Self‐Healing Cell Carrier for Bone Tissue Engineering

Vahedi

Shokrolahi

Barzin

et al. 2020

Macro Materials & Eng

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“…In a series work from the laboratory of Wang, thiolated‐hyaluronic acid was crosslinked with different hyperbranched polymers containing residue acrylate moieties via this thiol‐Michael addition, yielding hydrogels with promising applications in wound healing [ 120,239 ] and cartilage tissue engineering [ 240 ] after encapsulating cells. Various polymers including collagen, [ 124 ] hyaluronic acid, [ 241 ] dextran, [ 121 ] chondroitin sulfate, [ 122 ] were crosslinked by this chemistry and used for cell culture. This chemistry has also led to the FDA approved product—Premvia (K134037), which is crosslinked from three compounds: thiol–modified hyaluronan, thiol–modified gelatin, and PEG diacrylate.…”

Section: Crosslinking Chemistrymentioning

confidence: 99%

Covalently Crosslinked Hydrogels via Step‐Growth Reactions: Crosslinking Chemistries, Polymers, and Clinical Impact

2021

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Hydrogels are an important class of biomaterials with the unique property of high‐water content in a crosslinked polymer network. In particular, chemically crosslinked hydrogels have made a great clinical impact in past years because of their desirable mechanical properties and tunability of structural and chemical properties. Various polymers and step‐growth crosslinking chemistries are harnessed for fabricating such covalently crosslinked hydrogels for translational research. However, selecting appropriate crosslinking chemistries and polymers for the intended clinical application is time‐consuming and challenging. It requires the integration of polymer chemistry knowledge with thoughtful crosslinking reaction design. This task becomes even more challenging when other factors such as the biological mechanisms of the pathology, practical administration routes, and regulatory requirements add additional constraints. In this review, key features of crosslinking chemistries and polymers commonly used for preparing translatable hydrogels are outlined and their performance in biological systems is summarized. The examples of effective polymer/crosslinking chemistry combinations that have yielded clinically approved hydrogel products are specifically highlighted. These hydrogel design parameters in the context of the regulatory process and clinical translation barriers, providing a guideline for the rational selection of polymer/crosslinking chemistry combinations to construct hydrogels with high translational potential are further considered.

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“…That allowed for the formation of injectable hydrogels with shear-thinning and self-healing properties because of the low degree of thiol modification on collagen and the formation of an interhelical assembly. [82] Similarly, Wang et al described the synthesis of an injectable hydrogel made from thiolate-modified hyaluronic acid and quinonemodified polydopamine. When injected into heart muscle, this hydrogel was found to improve cardiac functions by decreasing inflammation.…”

Section: Chemically Crosslinked Injectable Hydrogelsmentioning

confidence: 99%

Recent Advances in Injectable Hydrogels for Controlled and Local Drug Delivery

Rizzo

Kehr

2020

Adv Healthcare Materials

222

152

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Injectable hydrogels have received considerable interest in the biomedical field due to their potential applications in minimally invasive local drug delivery, more precise implantation, and site‐specific drug delivery into poorly reachable tissue sites and into interface tissues, where wound healing takes a long time. Injectable hydrogels, such as in situ forming and/or shear‐thinning hydrogels, can be generated using chemically and/or physically crosslinked hydrogels. Yet, for controlled and local drug delivery applications, the ideal injectable hydrogel should be able to provide controlled and sustained release of drug molecules to the target site when needed and should limit nonspecific drug molecule distribution in healthy tissues. Thus, such hydrogels should sense the environmental changes that arise in disease states and be able to release the optimal amount of drug over the necessary time period to the target region. To address this, researchers have designed stimuli‐responsive injectable hydrogels. Stimuli‐responsive hydrogels change their shape or volume when they sense environmental stimuli, e.g., pH, temperature, light, electrical signals, or enzymatic changes, and deliver an optimal concentration of drugs to the target site without affecting healthy tissues.

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Injectable Shape-Holding Collagen Hydrogel for Cell Encapsulation and Delivery Cross-linked Using Thiol-Michael Addition Click Reaction

Cited by 78 publications

References 68 publications

Amylopectin Multiple Aldehyde Crosslinked Hydrogel as an Injectable and Self‐Healing Cell Carrier for Bone Tissue Engineering

Amylopectin Multiple Aldehyde Crosslinked Hydrogel as an Injectable and Self‐Healing Cell Carrier for Bone Tissue Engineering

Covalently Crosslinked Hydrogels via Step‐Growth Reactions: Crosslinking Chemistries, Polymers, and Clinical Impact

Recent Advances in Injectable Hydrogels for Controlled and Local Drug Delivery

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