Injectable, shear-thinning, photocrosslinkable, and tissue-adhesive hydrogels composed of diazirine-modified hyaluronan and dendritic polyethyleneimine

Abstract

In the present study, we report the first synthesis of diazirine-modified hyaluronic acid (HA-DAZ). In addition, we also produced a precursor polymer solution composed of HA-DAZ and dendritic polyethyleneimine (DPI)...

Functionalized Gelatin/Polysaccharide Hydrogels for Encapsulation of Hepatocytes

“…In this initial study, we investigated the formation of hydrogels based on dialdehydefunctionalized (bioactive) hyaluronic acid (oxHA) or (bioinert) alginic acid (oxALG) combined with hydrazide-functionalized gelatin (hGel). The hydrogel formation is primarily based on the reaction of hydrazide with aldehyde groups to form covalent hydrazone bonds, but also imine bond formation (with amine groups present in native gelatin) as a potential side reaction [36]. Besides the synthesis of the compounds, different ratios of oxidized polysaccharides with hGel were established and studied regarding their stability and mechanical properties to be applicable for embedding HepG2 cells.…”

“…Gelatin can form hydrogels with many different p ners, such as cellulose, chitosan, alginate, and hyaluronic acid, for different applicatio To form crosslinks between both components, different functionalization methods can employed. For example, the modification of hyaluronic acid with diazirine groups le to photo-cross-linkable, biocompatible, hybrid hydrogels with shear-thinning proper [36]. A widely used method is the Schiff base formation between the amine groups of gelatin and aldehyde groups of functionalized polysaccharides.…”