Injectable Sugar Beet Pectin/Chitosan Derivative Composite Hydrogel for Wound Care

Fukuhara, Yoshiki; Takei, Takayuki; Yoshinaga, Takuma; Nishimata, Hiroto; Yoshida, Masahiro

doi:10.1252/jcej.19we102

Cited by 3 publications

(1 citation statement)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, Takei and colleagues demonstrated the biocompatibility of the SBP hydrogels both in vitro and in vivo . Furthermore, composite hydrogels composed of SBP mixed with gelatin and chitosan-gluconic acid have been applied for wound healing and cancer drug delivery. , Although these findings highlight the considerable potential of SBP hydrogels, to date, there are no reports that applied this system in a 3D bioprinting system to fabricate cell-laden constructs.…”

Section: Introductionmentioning

confidence: 99%

3D Bioprinting of Sugar Beet Pectin through Horseradish Peroxidase-Catalyzed Cross-Linking

Mubarok,

Zhang,

Sakai

2024

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Horseradish peroxidase (HRP)-mediated hydrogelation, caused by the cross-linking of phenolic groups in polymers in the presence of hydrogen peroxide (H 2 O 2 ), is an effective route for bioink solidification in 3D bioprinting. Sugar beet pectin (SBP) naturally has cross-linkable phenols through the enzymatic reaction. Therefore, chemical modifications are not required, unlike the various polymers that have been used in the enzymatic cross-linking system. In this study, we report the application of SBP in extrusion-based bioprinting including HRPmediated bioink solidification. In this system, H 2 O 2 necessary for the solidification of inks is supplied in the gas phase. Cell-laden liver lobule-like constructs could be fabricated using bioinks consisting of 10 U/mL HRP, 4.0 and 6.0 w/v% SBP, and 6.0 × 10 6 cells/mL human hepatoblastoma (HepG2) cells exposed to air containing 16 ppm of H 2 O 2 concurrently during printing and 10 min postprinting. The HepG2 cells enclosed in the printed constructs maintained their viability, metabolic activity, and hepatic functions from day 1 to day 7 of the culture, which indicates the cytocompatibility of this system. Taken together, this result demonstrates the potential of SBP and HRP cross-linking systems for 3D bioprinting, which can be applied in tissue engineering applications.

show abstract

Section: Introductionmentioning

confidence: 99%